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Biologia Plantarum

, Volume 41, Issue 4, pp 497–505 | Cite as

Physical mapping of the 18S-25S and 5S ribosomal RNA genes in diploid bananas

  • M. Doleželová
  • M. Valárik
  • R. Swennen
  • J.P. Horry
  • J. Doležel
Article

Abstract

Fluorescent in situ hybridisation (FISH) was used to determine the number and distribution of the 18S-25S and 5S rDNA sites on mitotic chromosomes of 6 wild and 2 edible diploid (2n=22) accessions belonging to the two banana species, Musa acuminata and M. balbisiana. FISH with the 18S-25S probe resulted in signals on one pair of chromosomes, the position of signals corresponded to the secondary constriction at the end of a short arm. The intensity of labelling was different between the homologues and the larger site corresponded to a larger secondary constriction. This labelling pattern was observed consistently in all genotypes. On the other hand, differences in the number of 5S sites were observed between the accessions. While in some of the wild seeded species, the 5S rDNA was localised on two pairs of chromosomes, hybridisation signals appeared on three pairs of chromosomes in other wild accessions. Quite unexpectedly, only five sites of 5S rDNA were reproducibly observed in the two vegetatively propagated diploid edible cultivars, Pisang Mas and Niyarma Yik, evidence for structural heterozygosity. A dual colour FISH showed that in all accessions, the satellite chromosomes carrying the 18S-25S loci did not carry the 5S loci. The results demonstrate that molecular cytogenetics can be applied to Musa and that physical cytogenetic maps can be generated.

Musa acuminata Musa balbisiana fluorescent in situ hybridisation FISH 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • M. Doleželová
    • 1
  • M. Valárik
    • 2
  • R. Swennen
    • 3
  • J.P. Horry
    • 4
  • J. Doležel
    • 2
  1. 1.Academy of Sciences of the Czech Republic, Sokolovská 6Institute of Experimental BotanyOlomoucCzech Republic
  2. 2.Institute of Experimental Botany, Sokolovská 6De Montfort University Norman Borlaug Centre for Plant ScienceOlomoucCzech Republic
  3. 3.Laboratory of Tropical Crop ImprovementKatholieke Universiteit LeuvenHeverleeBelgium
  4. 4.International Network for the Improvement of Banana and PlantainMontpellierFrance

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