Direct Organogenesis in Hypocotyl Cultures of Tamarindus Indica
- 162 Downloads
Direct differentiation of shoot buds from hypocotyl segments of 12-d-old seedlings of Tamarindus indica was obtained on Murashige and Skoog (MS) medium with or without growth regulators. The highest regeneration (66 %) and the maximum number of shoots (3 - 4) per explant were obtained from the explants on MS medium containing 6-benzylaminopurine (5 × 10-6 M). A maximum roots per shoot were produced on medium containing 3-indole butyric acid (5 × 10-6 M). The resulting plantlets were hardened and transferred to soil in pots where 75 % of them survived and resumed growth. Histological examination of explants suggests that the shoots were of de novo origin which would make this system suitable for transformation experiments.
Unable to display preview. Download preview PDF.
- Bruning, J.L., Kintz, B.L.: Computational Handbook of Statistics.-Scott Foresman, Glenview 1977.Google Scholar
- Jaiwal, P.K. Gulati, A.: Micropropagation of Tamarindus indica L. from shoot tip and nodal explants.-Nat. Acad. Sci. Lett. 15: 63-67, 1992.Google Scholar
- Kackar, N.L., Solanki, K.R., Singh, M., Vyas, S.C.: Micropropagation of Prosopis cineraria L.-Indian J. exp. Biol. 29: 65-67, 1991.Google Scholar
- Kackar, N.L., Vyas, S.C., Singh, M., Solanki, K.R.: In vitro regeneration of Prosopis cineraria L. druce using root as explant.-Indian J. exp. Biol. 30: 429-430, 1992.Google Scholar
- Mascarenhas, A.F., Nair, S., Kulkarni, V.M., Agarwal, D.C., Khuspe, S.S., Mehta, U.J.: Tamarind.-In: Bonga, J.M., Durjan, D.J. (ed.): Cell and Tissue Culture in Forestry. Vol. 3. Case Histories: Gymnosperms, Angiosperms and Palms. Pp. 316-325. Martinus Nijhoff, Dordrecht 1987.Google Scholar
- Nataraja, K., Sudha Devi, A.M.: Induction of plantlets from seedling explants of Dalbergia latifolia Roxb. in vitro.-Beitr. Biol. Pflanz. 59, 341-350, 1985.Google Scholar
- Rao, R.G.V., Prasad, M.N.V.: Plantlet regeneration from the hypocotyl callus of Acacia auriculiformis -- multiple tree legume.-J. Plant Physiol. 137: 625-627, 1991.Google Scholar
- Shanker, S., Mohan Ram, H.Y.: Plantlet regeneration from tissue culture of Sesbania grandiflora.-Curr. Sci. 59: 1-10, 1990.Google Scholar
- Suman: In vitro clonal propagation of Dalbergia sissoo Roxb. -- a tree legume.-M. Phil. Thesis. M.D. University, Rohtak 1992.Google Scholar
- Trigiano, R.N., Geneve, R.L., Merkle, S.A., Preece, J.E.: Tissue and cell cultures of woody legumes.-Hort. Rev. 14: 265-332, 1993.Google Scholar
- Upadhayaya, S., Chandra, N.: Shoot and plantlet formation in organ and callus cultures of Albizzia lebbeck Benth.-Ann. Bot. 52: 421-424, 1983.Google Scholar