Biologia Plantarum

, Volume 41, Issue 3, pp 331–337 | Cite as

Direct Organogenesis in Hypocotyl Cultures of Tamarindus Indica

  • P.K. Jaiwal Sonia
  • A. Gulati
  • S. Dahiya
Article

Abstract

Direct differentiation of shoot buds from hypocotyl segments of 12-d-old seedlings of Tamarindus indica was obtained on Murashige and Skoog (MS) medium with or without growth regulators. The highest regeneration (66 %) and the maximum number of shoots (3 - 4) per explant were obtained from the explants on MS medium containing 6-benzylaminopurine (5 × 10-6 M). A maximum roots per shoot were produced on medium containing 3-indole butyric acid (5 × 10-6 M). The resulting plantlets were hardened and transferred to soil in pots where 75 % of them survived and resumed growth. Histological examination of explants suggests that the shoots were of de novo origin which would make this system suitable for transformation experiments.

plantlets leguminous tree tamarind 

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • P.K. Jaiwal Sonia
    • 1
  • A. Gulati
    • 1
  • S. Dahiya
    • 1
  1. 1.Department of BioSciencesM.D. UniversityRohtak -India

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