Antonie van Leeuwenhoek

, Volume 74, Issue 4, pp 229–235 | Cite as

Dipodascus capitatus, Dipodascus spicifer and Geotrichum clavatum: Genomic characterization

  • Maudy Th. Smith
  • G.A. Poot


The G+C contents of 25 strains of Dipodascus capitatus, Dipodascus spicifer and Geotrichum clavatum were found to be heterogeneous on basis of derivative graphs of the melting profiles. Strains showing similar derivative graphs of the melting curve exhibited high levels of DNA homology (80-100%); strains showing dissimilar derivative graphs exhibited low levels of DNA homology (5 to 45%). Being considered separate taxa on basis of these parameters, D. capitatus, D. spicifer and G. clavatum could be identified by a combination of the key characteristics growth on xylose, cellobiose, salicin and arbutin.

Dipodascus capitatus D.spicifer Geotrichum clavatum yeast taxonomy DNA heterogeneity 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aalbaek B, Stenderup J, Jensen HE, Valbak J, Nylin J & Huda A (1994) Mycotic and algal bovine mastitis in Denmark. APMIS 102: 451–456Google Scholar
  2. Arnold AG, Gribbin B, De Leval M, MacArtney F & Slack M (1981) Trichosporon capitatum causing fungal endocarditis. Thorax 36: 478–480Google Scholar
  3. Baird DR, Harris M, Menon R & Stoddart RW (1985) Systemic infection with Trichosporon capitatum in two patients with acute leukemia. Eur. J. Clin. Microbiol. 4: 62–64Google Scholar
  4. Barbor PRH, Rotimi VO & Fatani H (1995) Paravertebral abscess caused by Trichosporon capitatum in a child with acute lymphoblastic leukemia. J. Infection 31: 251–252Google Scholar
  5. de Hoog GS, Smith MTh & Gu & #x00E9;ho E (1986) A revision of the genus Geotrichum and its teleomorphs. Studies in Mycology 29: 1–131Google Scholar
  6. Gemeinhardt H (1965) Lungenpathogenit & #x00E4;t von Trichosporon capitatum beim Menschen. Zentralbl. Bakteriol. Parasitenkde. Infektionskr. Abt. 1, 196: 121–133Google Scholar
  7. Gu & #x00E9;ho E, de Hoog GS, Smith MTh & Meyer SA (1987) DNA relatedness, taxonomy, and medical significance of Geotrichum capitatum. J. Clinical Microbiology 25: 1191–1194Google Scholar
  8. Hellman E & Raethel S (1964) Trichosporon capitatum als Ursache eines Abortes beim Rind. Tier & #x00E4;rtztl. Wochenschr. 77: 380–381Google Scholar
  9. Heyndrickx M, Lebbe L, Vancanneyt M, Kersters K, De Vos P, Logan NA, Forsyth G, Nazli S, Ali N & Berkeley RC (1997) A polyphasic reassessment of the genus Aneurinibacillus, reclassification of Bacillus thermoaerophilus (Meier-Stauffer et al., 1996) as Aneurinibacillus thermoaerophilus comb. nov., and emended descriptions ofA. aneurinilyticus corrig., A. migulanus, and A.thermoaerophilus. Int. J. Syst. Bacteriol. 47: 808–817Google Scholar
  10. Jahnke K-D (1987) Assessing natural relationships by DNA analysis-Techniques and applications. Pp227–246. In: GS de Hoog, MTh Smith & ACM Weijman (Eds) The expanding realm of yeast-like fungi. Studies in Mycology 30: 1–510Google Scholar
  11. Johnson JL (1987) Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. Int. J. Syst. Bacteriol. 23: 308–315Google Scholar
  12. Kurtzman CP (1987) Prediction of biological relatedness among yeasts from comparisons of nuclear DNA complementarity. In: de Hoog GS, Smith MTh & Weijman ACM (Eds) The expanding realm of yeast-like fungi. Studies in Mycology 30: 359–468Google Scholar
  13. Kurtzman CP & Robnett CJ (1995) Molecular relationships among hyphal ascomycetous yeasts and yeastlike taxa. Can. J. Botany 73(1): S824–S830Google Scholar
  14. Kurtzman CP & Robnett CJ (1997) Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5& #x2032; end of the Large-Subunit (26S) ribosomal DNA gene. J. Clinical Microbiol. 35: 1216–1223Google Scholar
  15. Kurztman CP & Liu Z (1990) Evolutionary affinities of species assigned to Lipomyces and myxozyma estimated from ribosomal RNA sequence divergence. Current Microbiol. 21: 387–393Google Scholar
  16. Kurtzman CP, Smiley MJ, Johnson CJ & Wickerham LJ (1980) Two new closely related heterothallic species, Pichia amylophila and Pichia mississippiensis: characterization by hybridization and deoxyribonucleic acid reassociation. Int. J. Syst. Bacteriol. 30: 206–216Google Scholar
  17. Laaser G, Jahnke K-D, Prillinger H, Bauer R, Hoffman P, Deml G & Oberwinkler F (1988) A new tremalloid yeast isolated from Asterophora lycoperdoides (Bull.: Fr.) Ditm. Antonie van Leeuwenhoek 54: 57–74Google Scholar
  18. Pagano L, Morace G, Ortu-La Barbera E, Sanguinetti M & Leone G (1996) Adjuvant therapy with rhGM-CSF for the treatment of Blastoschizomyces capitatus systemic infection in a patient with acute myeloid leukemia. Ann. Hematol. 73: 33–34Google Scholar
  19. Perfect JR & Schell WA (1996) The new fungal opportunists are coming. Clin. Infect. Dis. 22 (Suppl.): S112–118Google Scholar
  20. Phaff HJ, Blue J, Hagler AN & Kurtzman CP. (1997) Dipodascus starmeri sp. nov., a new species of yeast occurring in cactus necroses. Int. J. Syst. Bacteriol. 47: 307–312Google Scholar
  21. Plum G, Scheid C, Franzen C, Sch & #x00FC;tt-Gerowitt H, Seifert H & Wickramanayake PD (1996) Empirical liposomal Amphoteracin-B therapy in a neurotropic patoent: Breakthrough of disseminated Blastoschizomyces capitatus infection. Zentralbl. Bakt. 284: 361–366Google Scholar
  22. Polachek I, Salkin IF, Kitzes-Cohen R & Raz R (1992) Endocarditis caused by Blastoschizomyces capitatus and taxonomic review of the genus. J. Clin. Microbiol. 30: 2318–2322Google Scholar
  23. Saëz H & Rinjard J (1973) Trichosporon capitatum, un constituant de la flore fongique du tube digestif de certains Suid & #x00E9;s. Ann. Med. Vet. 117: 177–182Google Scholar
  24. Salkin IF, Gordon MA, Samsonoff WA & Rieder CL (1985) Blastoschizomyces capitatus, a new combination. Mycotaxon 22: 375–380Google Scholar
  25. Seidler RJ & Mandel M(1971) Quantitative aspects of deoxyribonucleic acid renaturation: base composition, site of chromosome replication, and polynucleotide homologies. J. Bacteriol. 106: 608–614Google Scholar
  26. Smith MTh, de Cock AWAM, Poot GA & Steensma HY (1995) Genome comparisons in the yeastlike fungal genus Galactomyces Redhead et Malloch. Int. J. Syst. Bacteriol. 45: 826–831Google Scholar
  27. Stackebrandt E & Goebel M (1994) Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44: 846–849Google Scholar
  28. van der Walt, JP & Yarrow D (1984) Methods for isolation, maintenance, classification and identification of yeasts. In: NJW Kreger-van Rij (ed) The yeasts: a taxonomic study. 3rd ed. (pp 45–104) Elsevier Science Publishers, AmsterdamGoogle Scholar
  29. von Arx JA, Rodrigues de Miranda L, Smith MTh & Yarrow D (1977) The genera of yeasts and the yeast-like fungi. Studies in Mycology 14: 1–42Google Scholar
  30. Weijman ACM (1979) Carbohydrate composition and taxonomy of Geotrichum, Trichosporon and allied genera. Antonie van Leeuwenhoek 45: 119–127Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Maudy Th. Smith
    • 1
  • G.A. Poot
    • 1
  1. 1.Yeast DivisionCentraalbureau voor SchimmelculturesDelftThe Netherlands

Personalised recommendations