Antonie van Leeuwenhoek

, Volume 71, Issue 3, pp 195–200 | Cite as

Evaluation of Biolog system for identification of strains of Paenibacillus azotofixans

  • Marcelo Nolla Pires
  • Lucy Seldin


Biolog system was evaluated for the identification of strains of Paenibacillus azotofixans as no data concerning this species were in the list of Bacillus currently identified using Biolog data base. The P. azotofixans type strain P3L5 was first tested with the results recorded manually or using the automatic plate reader. In both cases, P3L5 utilized 22 carbon sources and when the results obtained were compared to data of Biolog software (Release 3.50), P3L5 was identified as B. azotoformans with a similarity coefficient of 0.913 (data recorded manually) and of 0.791 (data recorded automatically). Metabolic profiles of P3L5 were also compared after readings of 4 and 24 h using the computer-driven automatic plate reader. No significant difference was observed in both cases and P3L5 was identified again as B. azotoformans with indices of similarity considered only for ‘excellent identification’. Besides P3L5, other 15 P. azotofixans strains were tested with the Biolog system and all were identified as B. azotoformans with similarity coefficients varying from 0.511 to 0.927. Phenotypic and genetic characteristics of B. azotoformans were compared to those described for P. azotofixans to explain the misidentification of the latter species. We could conclude that these two species are quite different and that data of Biolog software are from P. azotofixans and not from B. azotoformans.

Biolog system Paenibacillus azotofixans identification 


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Copyright information

© Kluwer Academic Publishers 1997

Authors and Affiliations

  • Marcelo Nolla Pires
    • 1
  • Lucy Seldin
    • 1
  1. 1.Laboratório de Genética MicrobianaInstituto de Microbiologia da Universidade Federal do Rio de Janeiro, C.C.S.Rio de Janeiro, RJBrasil

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