Skip to main content
SpringerLink
Log in

Effects of Anti-Juvenile Hormone Agents and a Juvenile Hormone Analog on Neck-Ligated Post-Feeding Last Instar Larvae of Spodoptera Mauritia Boisd. (Lepidoptera:Noctuidae)

  • Research Article
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

The ability of three anti-juvenile hormone agents (AJHAs) to prevent the juvenile hormone dependent phase of larval-pupal transformation has been investigated. Neck-ligated post-feeding last instar larvae of Spodoptera mauritia were treated topically with different doses of a juvenile hormone analog (JHA) or the AJHAs, tetra-hydro-4-fluoromethyl-4-hydroxy-2H-pyran-2-one (FMev), ethyl-4-[2-(tert-butyl carbonyloxy)butoxy] benzoate (ETB) and ethyl-[E]-3-methyl-2-dodecanoate (EMD). Untreated ligated larvae or those treated with acetone survived for 12 ± 1 days without showing any sign of pupal cuticle secretion. On the other hand treatments of ligated larvae with different doses of JHA induced pupation. Among the three AJHAs tested treatments of ETB promoted pupation. Treatments of ligated larvae with lower doses of EMD induced the formation of larval-pupal intermediates whereas those treated with higher dose moulted into either pupae or larval-pupal intermediates. FMev-treated larvae exhibited a complete inhibition of moulting and metamorphosis. In order to study whether AJHAs would prevent the JHA induced pupation, neck-ligated larvae were treated simultaneously with different doses of AJHAs and 1μg JHA. Co-application of JHA with different doses of EMD induced pupation in majority of the ligated larvae and thus appears to a certain extent to counteract the effects of treatments of same doses of EMD alone. Co-application of JHA with different doses of ETB elicited pupation in all the surviving ligated larvae. To a large extent co-application of JHA with different doses of FMev failed to induce pupation. The significance of the findings is discussed in the context of the mode of action of these AJHAs.

Résumé

Les trois (AJHAs) ont été examinés son pouvoir qu’ empeche la transformation du Juvenile hormone dependent phase de larvaere-pupae. Ayant nourir dernière stade cou-ligaturés larvaire de Spodoptera mauritia sont traité topicalement avec en doses differements du Juvenile hormone analog (JHA) ou AJHAs, tetra-hydro-4-fluoromethyl-4-hydroxy-2H-pyran-2-one (FMev), ethyl-4-[2-(tert-butyl carbonyloxy)butoxy] benzoate (ETB) et ethyl-[E]-3-methyl 2-dodecanoate (EMD). Non traité larvaire ligaturés ou celles sont traité avec acetone survie pendant 12 ± 1 jours sans aucune signe de secretion par pupal cuticle. D’ailleurs traitement des larvaires ligaturés avec en doses differents de JHA induit pupation. Parmi les larvaire déjà traité par trois AJHAs sont induit pupation par ETB. Traitment des larvaires ligaturés avec en doses inferieurs du EMD induit la formation de larvaires-pupal intermediares tandisque celles qui sont traités en doses superieurs mue a pupae ou larvaires pupal intermediares. Larvaires traités par FMev montre une inhibition complete du mue et metamorphosis. A fin d’étudier si AJHAs empechevont JHA induit pupation, cou-ligaturé larvaires sont traité simultanement avec en doses differents des AJHAs et 1μg JHA. En majorité des larvaires ligaturés induit pupation par co-traitment de JHA avec en doses differents de EMD. Co-traitment de JHA avec en doses differents de ETB elicit pupation dans toutes les larvaires ligaturés survivant. En plus part des cas co-traitment de JHA avec en doses differents de FMev n’induit pas la pupation. Les significances des resultats ont discutes d’une mannière de la mode d’action des AJHAs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Balamani E. and Nair V.S.K. (1989) Prothoracicotropic action of a juvenile hormone analogue on ncck-ligated last instar larvae of Spodoptera mauritia Boisd. (Lepidoptera: Noctuidae). Entomon (In press).

    Google Scholar 

  • Bean D.W., Beck S.D., and Goodman W.G. (1982) Juvenile hormone esterases in diapause and non-diapause larvae of the European corn borer Ostrinia nubilalis. J. Insect Physiol. 28, 485–492.

    Article  CAS  Google Scholar 

  • Bollenbacher W.E., Smith S.L., Goodman G. and Gilbert L.I. (1981) Ecdysteroid titer during larval-pupal-adult development of the tobacco hornworm, Manduca sexta. Gen. Comp. Endocrinol. 44, 302–306.

    Article  CAS  Google Scholar 

  • Bowers W.S. (1982) Toxicology of the precocenes. In Insecticide Mode of Action (Edited by Coats J.R.), pp. 403–427. Academic Press, New York.

    Chapter  Google Scholar 

  • Cymborowski B. and Stolarz G. (1979) The role of juvenile hormone during larval-pupal transformation in Spodoptera littoralis: Switch over in the sensitivity of the prothoracic glands to juvenile hormone. J. Insect Physiol. 25, 939–942.

    Article  CAS  Google Scholar 

  • Dean R., Bollenbacher W.E., Locke M., Smith S.L. and Gilbert L.I. (1980) Haemolymph ecdysteroid levels and cellular events in the intermoult/moult sequence of Calpodes ethlius. J. Insect Physiol. 26, 267–280.

    Article  CAS  Google Scholar 

  • Denlinger D.L. (1979) The physiology of pupal diapause in flesh flies. In Current Topics in Insect Endocrinology and Nutrition (Edited by Bhaskaran G., Friedman S. and Rodriguez J.G.), pp. 131–160. Plenum Press, New York.

    Google Scholar 

  • Gruetzmacher M.C., Gilbert L.I., Granger N.A., Goodman W. and Bollenbacher W.E. (1984) The effect of juvenile hormone on prothoracic gland function during larval-pupal development of Manduca sexta: An in situ and in vitro analysis. J. Insect Physiol. 30, 331–340.

    Article  CAS  Google Scholar 

  • Hiruma K. (1980) Possible roles of juvenile hormone in the prepupal stage of Mamestra brassicae. Gen. Comp. Endocrinol. 41, 392–400.

    Article  CAS  Google Scholar 

  • Hsiao T.H. and Hsiao C. (1977) Simultaneous determination of moulting and juvenile hormone titers of the greater wax moth. J. Insect Physiol. 23, 89–93.

    Article  CAS  Google Scholar 

  • Jones G. and Hammock B.D. (1985) Critical roles for prepupal juvenile hormone and its esterase. Arch. Insect Biochem. Physiol. 2, 397–404.

    Article  CAS  Google Scholar 

  • Kondo K. (1973) β-phenoxy-oder substituierte phenoxyethanol-verbindungen offenlegung-schrift 24 56082, Bundesrepublik Deutschland, May 28, 1973.

    Google Scholar 

  • Maroy P. and Tarnoy K. (1978) Moulting hormone level during the last instar of Gallaria mellonella larva. J. Insect Physiol. 24, 325–327.

    Article  CAS  Google Scholar 

  • Monger D.J., Lim W.A., Kezdy F.J., and Law J.H. (1982) Compactin inhibits insect HMG CoA reductase and juvenile hormone biosynthesis. Biochem. Biophys. Res. Commun. 105, 1374–1380.

    Article  CAS  Google Scholar 

  • Murakoshi S., Nakata T., Ohtsuka Y., Akita H., Tahara A. and Tamura S. (1975) Appearance of three-moulters from larvae of silkworm, Bombyx mori L. by oral administration of abeitic acid derivatives. Jap. J. appl. Entomol. Zool. 19, 267–272.

    Article  CAS  Google Scholar 

  • Nair V.S.K. (1981) Hormonal control on the mating behaviour in the females of Spodoptera mauritia (Lepidoptera: Noctuidae). Curr. Sci. 50, 690–691.

    Google Scholar 

  • Nair V.S.K. and Rajalekshmi E. (1989) Effects of fluoromevalonate on the penultimate and last instar larvae of Spodoptera mauritia Boisd. (Lepidoptera: Noctuidae). Ind. J. Exp. Biol. 27, 170–173.

    CAS  Google Scholar 

  • Quistad G.B., Cerf D.C., Schooley D.A. and Staal G.B. (1981) Fluoromevalonate acts as an inhibitor of insect juvenile hormone biosynthesis. Nature 289, 176–177.

    Article  CAS  Google Scholar 

  • Riddiford L.M. (1985) Hormone action at cellular level. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Edited by Kerkut G.A. and Gilbert L.I.), Vol. 8, pp. 37–74. Pergamon Press, Oxford.

    Google Scholar 

  • Riddiford L.M., Roseland G.R., Thalberg S. and Curtis A.T. (1983) Action of two juvenile hormone antagonists on lepidopteran epidermis. J. Insect Physiol. 29, 281–286.

    Article  CAS  Google Scholar 

  • Santha P.C. and Nair V.S.K. (1986) Age-dependent responses of last instar larva of Spodoptera mauritia to precocene II. Physiol. Entomol. 11, 335–341.

    Article  CAS  Google Scholar 

  • Schooley D. A., Judy K. J., Bergot B.J., Hall M.S. and Jennings R.C. (1976) Determination of the physiological levels of juvenile hormones in several insects and biosynthesis of the carbon skeletons of the juvenile hormones. In The Juvenile Hormones (Edited by Gilbert L.I.), pp. 101–117. Plenum Press, New York.

    Chapter  Google Scholar 

  • Sieber R. and Benz G. (1980) The hormonal regulation of the larval diapause in the codling moth Lasperesia pomonella (Lepidoptera: Tortricidae). J. Insect Physiol. 26, 213–218.

    Article  CAS  Google Scholar 

  • Sparks T.C. (1984) Effects of juvenile hormone I and the anti-juvenile hormone fluoromevalonolac-tone on development and juvenile hormone esterase activity in post feeding last-stadium larvae of Trichoplusia ni (Hübner). J. Insect Physiol. 30, 225–234.

    Article  CAS  Google Scholar 

  • Sparks T.C., Wing K.D., and Hammock B.D. (1979) Effects of the anti juvenile hormone mimic ETB on the induction of insect juvenile hormone esterases in Trichoplusia ni. Life Sci. 25, 445–450.

    Article  CAS  Google Scholar 

  • Sparks T.C., Roe R.M., Buehler A., and Hammock B.D.(1987) The evaluation of anti juvenile hormones using last stadium larvae of the cabbage looper Trichoplusia ni (Hübner). Insect Biochem. 17, 1011–1016.

    Article  CAS  Google Scholar 

  • Staal G.B. (1986) Anti juvenile hormone agents. A. Rev. Entomol. 31, 391–426.

    Article  CAS  Google Scholar 

  • Staal G.B., Henrick C.A., Bergot B.J., Cerf D.C., Edwards J.P. and Kramer S.J. (1981) Relationships and interactions between JH and anti-JH analogues in Lepidoptera. In Regulation of Insect Development and Behaviour (Edited by Sehnal F., Zabza A., Menn J. J. and Cymborowski B.), pp. 324–340. Wroclaw Technical University Press, Poland.

    Google Scholar 

  • Van Mellaert H., De Loof A. and Jurd L. (1983) Anti-juvenile hormone effects of newly described chemosterilants: Benzyl-1,3-benzodioxoles and benzyl phenols. Entomol. Exp. Appl. 33, 83–88.

    Article  Google Scholar 

  • Varjas L., Paguia P. and De Wilde J. (1976) Juvenile hormone titers in penultimate and last instar larvae of Pieris brassicae and Barathra brassicae in relation to the effect of juvenoid application. Experientia 32, 249–251.

    Article  CAS  Google Scholar 

  • Willis J.H. (1974) Morphogenetic action of insect hormones. A. Rev. Entomol. 19, 97–115.

    Article  CAS  Google Scholar 

  • Zimowska G., Handler A.M. and Cymborowski B. (1985) Cellular events in the prothoracic glands and ecdysteroid titres during the last-larval instar of Spodoptera littoralis. J. Insect Physiol. 31, 331–340.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Zoology, University of Calicut, Kerala, 673 635, India

    E. Balamani & V. S. K. Nair

Authors
  1. E. Balamani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. S. K. Nair
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Balamani, E., Nair, V.S.K. Effects of Anti-Juvenile Hormone Agents and a Juvenile Hormone Analog on Neck-Ligated Post-Feeding Last Instar Larvae of Spodoptera Mauritia Boisd. (Lepidoptera:Noctuidae). Int J Trop Insect Sci 10, 583–589 (1989). https://doi.org/10.1017/S1742758400021706

Download citation

  • Received:

  • Revised:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1017/S1742758400021706

Key Words

Search

Navigation