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Genetic Analysis of Brown Planthopper Biotypes Using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)

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Abstract

Random amplified polymorphic DNA (RAPD) amplified by the polymerase chain reaction (PCR) was used to estimate the relatedness of three biotypes of brown planthopper, Nilaparvata lugens (Stål), from the Philippines. Each individual brown planthopper had a unique set of RAPD bands, but no bands were diagnostic for any one biotype. Brown planthopper types 1, 2 and 3 were genetically homogeneous. This supports results of other studies which conclude that brown planthopper biotypes are not sub-specific categories, but merely represent individual variation for virulence to rice cultivars.

Résumé

Le random amplified polymorphic DNA amplifié au PCR (RAPD-PCR) a été utilisé pour determiner les liens de parenté de trois biotypes de la sauterelle brune, Nilaparvata lugens (Stål), provenant des Philippines. Chaque sauterelle brune a un lot unique de band RAPD mais aucune bande n’a été identifiée pour un quelconque biotype. Les biotypes 1, 2 et 3 de la sauterelle brune était génétiquement homogenes. Ce resultat est en accord avec ceux d’autres études qui ont conclu que les biotypes de la sauterelle brune ne sont pas des catégories subspécifiques mais ils représentent plutôt des variations individuelles de virulence à l’encontre des cultivars de riz.

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Author notes

  1. K. A. Shufran

    Present address: USDA, ARS, 1301 N. Western St., Stillwater, OK, 74075, USA

Authors and Affiliations

  1. Department of Entomology and Pesticide Research Center, Michigan State University, East Lansing, Michigan, 48824, USA

    K. A. Shufran & M. E. Whalon

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  1. K. A. Shufran
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  2. M. E. Whalon
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Shufran, K.A., Whalon, M.E. Genetic Analysis of Brown Planthopper Biotypes Using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). Int J Trop Insect Sci 16, 27–33 (1995). https://doi.org/10.1017/S1742758400018282

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  • DOI: https://doi.org/10.1017/S1742758400018282

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