Abstract
Genetic differentiations in natural populations of the tsetse fly, Glossina pallidipes Austen (Diptera: Giossinidae) collected from three ecologically distinct regions of Kenya, namely Lambwe Valley, Shimba Hills and Nguruman were analysed based on two enzymes, phosphoglucomutase (PGM) and phosphoglucose isomerase (GPI). The PGM gene frequencies of allele (a) of male and female flies from Lambwe Valley showed significant differences. A dendrogram of populations collected from different localities showed two clusters: one comprising Shimba Hills, Majimboni and Muhaka Forest and the other comprising Gendo, Homa Hills and Nguruman. This suggests mat different subpopulations of G. pallidipes have adapted to different habitats.
Résumé
Des différentiations génétiques chez des populations naturelles de Glossina pallidipes Austen (Diptera: Giossinidae) receuillies dans la vallée de Lambwe, à Shimba Hills et à Nguruman, Kenya ont été analysées en se basant sur deux enzymes, la phosphoglucomutase (PGM) et la phosphoglucose isomerase (PGI). La fréquence du gène PGM de l’allèle (a) des mouches mâles et femelles en provenance de la Vallée de Lambwe a montré des différences significatives. Un dendrograme obtenu par UPGMA des populations provenant de différentes localités a mis en évidence 2 clusters, l’un comprennant Shimba Hills, Majimboni, Muhaka Forest et le second comprennant Gendo, Homa Hills et Nguruman. Ce résultat suggère que différentes sous-populations de G. pallidipes se sont adaptées aux différentes habitats.
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References
Agatsuma T. and Otieno L. H. (1988) Isozyme studies on two field populations of Glossina pallidipes Austen (Diptera, Glossinidae) in Kenya. Insect Sci. Applic. 9, 527–530.
Divall G. B. (1984) Starch gel electrophoresis of proteins. In Methods in Moleculer Biology. 1. Proteins (Edited by J. M. Walker). Humana Press Inc. US.
Gooding R. H. (1984) Tsetse genetics: A review. Quaest. Ent. 20, 89–128.
Gooding R. H. (1992) Genetic variation in tsetse flies and implications for trypanosomosis. Parasitology Today 8, 92–95.
Gooding R. H., Mbise S., Macha P. and Rolseth B. M. (1993) Genetic variation in a Tanzanian population of Glossina swynnertoni (Diptera: Glossinidae). J. Med. Entomol. 30, 489–492.
Gooding R. H. and Moloo K. S. (1994) Genetics of two colonies of Glossina pallidipes originating from allopatric populations in Kenya. Med. Vet. Entomol. 8, 133–136.
Gooding R. H. and Rolseth B. M. (1992) Genetics of Glossina morsitans morsitans (Diptera: Glossinidae). XIV. Map locations of the loci for phosphoglucomutase and glucose-6-phosphate isomerase. Genome 35, 699–701.
Harris H. and Hopkinson D. A. (1976) Handbook of Enzyme Electrophoresis in Human Genetics. North-Holland, Amsterdam.
ICIPE (1989) Report of preparatory phase of the project on trypanosomosis control in the Kagera River Basin (Raf/87/132). Sixteenth Annual Report, 1988.
Nei M. (1987) Molecular Evolutionary Genetics. Columbia University Press, New York. 521 pp.
Rajendram G. F. (1991) Electrophoretic study of enzymes from Glossina fuscipes fuscipes Newstead population from Western Kenya. Can. Ent. 123, 295–298.
Rolseth B. M. and Gooding R. H. (1978) Genetics of Glossina morsitans morsitans (Diptera: Glossinidae). I. Electrophoretic banding patterns of xanthine and aldehyde oxidase. Can. Ent. 110, 1233–1239.
Shaw C. R. and Prasad R. (1970) Starch gel electrophoresis—A compilation of recipes. Biochem. Genet. 4, 297–320.
Sneath P. H. A. and Sokal R. R. (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification. W. H. Freeman and Company, San Francisco.
Turner D. A. (1984) A preliminary assessment of some immediate and long-term effects of aerial spraying of endosulfan on Glossina pallidipes (Austen) in the Lambwe Valley, Kenya. Insect Sci. Applic. 5, 425–429.
Van Etten J. (1982a) Enzyme polymorphism in populations of the tsetse fly Glossina pallidipes in Kenya. Entomol. Exp. Appl. 31, 197–201.
Van Etten J. (1982b) Comparative studies on fat reserves. Feeding and metabolic strategies of flies from two allopatric populations of Glossina pallidipes Austen in Kenya. Acta Tropica 39, 157–169.
Van Etten J. (1982c) Comparative studies on the diurnal activity pattern in two field and laboratory populations of Glossinapallidipes. Entomol. Exp. Appl. 32, 38–45.
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Kence, A., Otieno, L.H., Darji, N. et al. Genetic Polymorphisms in Natural Populations of Tsetse Fly, Glossina pallidipes Austen in Kenya. Int J Trop Insect Sci 16, 369–373 (1995). https://doi.org/10.1017/S1742758400017422
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DOI: https://doi.org/10.1017/S1742758400017422