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The Effect of Photoperiod on the Haemolymph Protein Profile in The Silkworm, Bombyx mori L.

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Abstract

—The effect of photoperiod on the haemolymph protein profile in the silkworm, Bombyx mori L. larvae was investigated at photoperiod treatments of light to dark (L:D) ratios of 13:11 (control), 24:0, 18:6, 12:12, 6:18 and 0:24 hours using fifth instar larvae. The intensity of protein bands in haemolymph extracts from larvae was measured with a densitometer. The intensity was highest in the haemolymph from larvae in the 24:0 (L:D) h photoperiod. Strongly reactive antigens of SP-1 and SP-2 and weakly reactive bands of storage proteins for both male and female larvae were observed in the 24:0 (L:D) h photoperiod.

Résumé

—L’effet de la photopériode sur le profil protéinique de l’haemolymphe chez la chenille du ver-à-soie, Bombyx mori L. soumise aux traitements photopériodiques de 13L:11D (témoin), 24:0,18:6, 12:12, 6:18 et 0:24 h a été étudié au niveau des larves du 5ème instar. L’intensité des bandes de protéines dans des extraits d’haemolymphe des larves a été mesurée à l’aide d’un densimètre. La plus forte intensité a été notée au niveau de l’haemolymphe des larves à la photopériode de 24:0 (L:D). Des antigènes fortement réactifs des bandes SP-1 et SP-2 et des bandes faiblement réactives des protéines de stockage pour les larves mâles et femelles ont été observées à la photopériode de 24:0 (L:D).

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References

  • Beck S. D. (1980) Insect Photoperiodism. 2nd edn. Academic Press, New York.

    Google Scholar 

  • Benchamin K. V., Gapuz V. and Jayaramaraju P. (1990) Influence of photoperiod on emergence, fecundity and fertility in multivoltine breeds of silkworm, Bombyx mori L. Indian J. Seri. 29, 110–118.

    Google Scholar 

  • Danilevskii A. S. (1965) Photoperiodism and Seasonal Development of Insects. 1st English edn., Oliver and Boyd, Edinburgh.

    Google Scholar 

  • El. Shaarawy M. F., Gima A. A. and Megalla A. H. (1978) Reaction of photoperiodism on the silkworm, Bombyx mori—2. Consumption, digestion and utilisation of food. Z. angew Zool. 65, 425–434.

    Google Scholar 

  • Hirasaka T. and Koyama N. (1972) Effect of photoperiodic conditions on the larval growth of silkworm, Bombyx mori L. III Relationship between circadian photoperiods and the larval growth. J. Seri. Sci., Japan 41, 69–78.

    Google Scholar 

  • Janarthanan S., Sathiamoorthi M., Subburathinam K. M. and Krishnan M. (1994) Photoperiod: Its effects on larval-pupal characters, fat body nucleic acids and protein of silkworm, Bombyx mori L. Insect Sci. Applic. 15, 129–137.

    CAS  Google Scholar 

  • Kogure M. (1933) The influence of light and temperature on certain characters of silkworm, Bombyx mori. J. Dep. Agric., Kyushu University 4, 1–93.

    Google Scholar 

  • Laemmli U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227, 680–685.

    Article  CAS  Google Scholar 

  • Lakhotia S. C. and Singh B. N. (1993) A simple and inexpensive “Western-blotting” apparatus. Indian J. Exp. Biol. 31, 301–302.

    CAS  Google Scholar 

  • Levenbook L. (1985) Insect storage proteins. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Edited by Kerkut G. A. and Gilbert L. I.), 10, 307–346. Pergamon Press.

    Google Scholar 

  • Mahanta H. C. and Choudhury K. (1989) Role of temperature and photoperiod on growth and development of two Agrotis species (Lepidoptera: Noctuidae). Nutri. Ecol. Ins. Env. 142–150.

    Google Scholar 

  • Nargata M. and Kobayashi J. (1990) Quantitative changes in storage proteins during larval development of the silkworm, Bombyx mori. J. Seri. Sci., Japan 59, 461–468.

    Google Scholar 

  • Nijhout H. F. and Williams C. M. (1974) Control of moulting and metamorphosis in the tobacco hornworm, Manduca sexta (L): Growth of the last-instar larva and the decision to pupate. J. Exp. Biol. 611, 481–491.

    Google Scholar 

  • Pickens L. G. and Miller R. W. (1980) Biology and control of the face fly, Musca autumnalis (Diptera: Muscidae). J. Med. Entomol. 17, 195–210.

    Article  Google Scholar 

  • Sambrook J., Fritsch E. F. and Maniatis T. (1989) Molecular Cloning. 2nd Edn. Cold Spring Harbor Laboratory Press, New York 18, 3.

    Google Scholar 

  • Tojo S., Nagata M. and Kobayashi M. (1980) Storage proteins in the silkworm, Bombyx mori. Insect Biochem. 10, 289–303.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biotechnology, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    S. Janarthanan, K. M. Subbu Rathinam & M. Krishnan

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  1. S. Janarthanan
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  2. K. M. Subbu Rathinam
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  3. M. Krishnan
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Janarthanan, S., Subbu Rathinam, K.M. & Krishnan, M. The Effect of Photoperiod on the Haemolymph Protein Profile in The Silkworm, Bombyx mori L.. Int J Trop Insect Sci 16, 177–182 (1995). https://doi.org/10.1017/S1742758400017082

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  • DOI: https://doi.org/10.1017/S1742758400017082

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