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Effect of Protein Fractions Isolated from Cereal Grains on the Development and Survival of the Cowpea Weevil, Callosobruchus maculatus

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Abstract

—The effect of protein fractions isolated from cereal grains on the development and survival of the bean weevil, Callosobruchus maculatus, was investigated using wheat, rice and maize. These cereals were fractionated into albumins, globulins, prolamins, glutelins and a residue. The fractions were incorporated in artificial seeds which were infested by C. maculatus. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-larvae of the bruchid were negatively affected by most of the fractions. Rice globulins were the most detrimental and affected both the survival and development of the larvae, while wheat albumins and globulins had a stronger effect on larval development than on survival. A previously isolated maize α-amylase/trypsin inhibitor showed an LD50 of 1.5% to Callosobruchus maculatus.

Résumé

—L’effet des fractions protéiniques des semences de blé de riz et de maïs a été étudié sur le développement et la survie du bruchide du niébé, Callosobruchus maculatus. Les céréales ont été fractionnées en albumines, globulines, prolamines, glutélines et en un résidu, avec les fractions incorporées dans des semences artificielles infestées du bruchide, Callosobruchus maculatus. La survie (telle qu’indiquée par la CL50) et le développement (tel qu’indiqué par WD50) des larves a âgées de 20 jours ont été négativement affectés par la plupart des fractions. Les globulines du riz ont été les plus nocives, affectant à la fois la survie et le développement des larves, tandis que l’effet des albumines et de globulines du blé a été plus marqué sur le développement larvaire que sur la survie. Un inhibiteur de la α-amylase/trypsine auparavant isolé du maïs, a révélé une CL50 de 1,5% sur Callosobruchus maculatus.

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Author notes

  1. M. P. Sales & J. Xavier-Filho

    Present address: Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, 28015-620, Campos dos Goytacazes, Rio de Janeiro, Brazil

Authors and Affiliations

  1. Grupo de Biotecnologia de Plantas Cultivadas, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Caixa Postal 1065, 60001-970, Fortaleza, Ceaá, Brazil

    L. B. S. Andrade, V. M. Q. Flores, M. P. Sales, R. A. Morais & J. Xavier-Filho

  2. Centro de Investigacion y de Estudios Avanzados del I.P.N— Unidad Irapuato, Carretera Irapuato-Leon, Irapuato, Gto, Mexico

    A. Blanco-Labra

Authors
  1. L. B. S. Andrade
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  2. V. M. Q. Flores
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  3. M. P. Sales
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  4. R. A. Morais
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  5. A. Blanco-Labra
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  6. J. Xavier-Filho
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Andrade, L.B.S., Flores, V.M.Q., Sales, M.P. et al. Effect of Protein Fractions Isolated from Cereal Grains on the Development and Survival of the Cowpea Weevil, Callosobruchus maculatus. Int J Trop Insect Sci 16, 171–176 (1995). https://doi.org/10.1017/S1742758400017070

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