Skip to main content
SpringerLink
Log in

Synthesis and Transport of RNA in the Salivary Glands of Some Bugs (Heteroptera)

  • Research Article
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

Autoradiographic studies using 3H uridine in the salivary glands of three bugs, Lethocerus indicus, Laccotrephes rubra and Antilochus coqueberti show that the nuclei play an important role in contributing RNA. The labelled materials are seen to move into the lumen from the epithelia. RNA shows a tendency to accumulate in the lumen with ascending incubation periods. This behaviour in conjunction with its nucleolar genesis indicates its nature as rRNA. It is also believed that RNA might conjugate with some of the protein available in the lumen to produce RNP.

Résumé

Desetudes autoradiographiques des glandes salivaires de 3 espèces de punaises, Lethocerus indicus, Laccotrephes rubra et Antilochus coqueberti, a l’aide de l’uridine radioactive (3H) montrent que les nuclei jouent un rôle primordial dans la synthèse de l’ARN. Le matériel radioactif semble se déplacer de i’épithélium vers la lumière. L’ ARN montrerait une tendance à s’accumuler dans la lumière au fur et à mesure que se prolongent les périodes d’incubation. Ce fait, en conjonction avec l’origine nucléique de l’ARN confirme que l’ARN en question ici est d’origine ribosomal. On suppose aussi que l’ARN se combinerait avec certaines protéines présentes dans la lumière pour former des PRN.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akai H. and Kobayashi M. (1965a) Site of the fibroin formation in the silk glands of Bombyx morì. Nature 206, 529.

    Article  Google Scholar 

  • Akai H. and Kobayashi M.(1965b) Incorporation of the labelled thymidine into the silk glands of the silkworm. Nature 206, 847–848.

    Article  CAS  Google Scholar 

  • Beams H. and Sekhon S. (1966) Morphological studies on the secretion in the silk glands of the caddish fly larvae, Platyphalyx designatus. Z. Zellforsh 72, 408–414.

    Article  CAS  Google Scholar 

  • Berry S. J. (1985) RNA synthesis and storage during insect oogenesis. In Developmental Biology. A Comprehensive Synthesis Vol. 1 Oogenesis (Edited by Browder L. W.), Plenum Press, N. York.

    Google Scholar 

  • Bier K.(1963) Synthese, interzellularev Transport und Abbu von Ribonucleinsaure in Ovar der Stubenfliege Musca domestica. J. Cell Biol. 16, 436-440.

    Google Scholar 

  • Capco D. and Jeffery W., (1979) Origin and special distribution of maternal mRNA during oogenesis of an insect Oncopeltus fasciatus. J. Cell Sci. 39, 63–76.

    CAS  PubMed  Google Scholar 

  • Cave M.D. (1973) Synthesis and characterisation of amplified DNA oocytes of the house cricket, Achaeta domesticus (Orthoptera: Gyrillaidae). Chromosoma 42, 1–22.

    Article  CAS  Google Scholar 

  • Gundevia H.S (1985) Autoradiographic studies on RNA synthesis and transport in the ovary of Hydrophylus olivaceus (Coleoptera). Experientia 41, 383–385.

    Article  CAS  Google Scholar 

  • Gundevia H.S. (1989) Studies on the RNA synthesis and transport in the corpora aliata of Hydrophylus olivaceus Fabr. (Coleoptera). Zool. Jb. Physiol. 93, 347–352.

    CAS  Google Scholar 

  • Haridass E.T, and Ananthakrishnan T. N. (1981) Functional morphology of the salivary system in some Reduviidae (Insecta:Heteroptera), Proc. Indian Acad. Sci. (Anim. Sci.) 90, 145–160.

    Article  Google Scholar 

  • Jacob J. (1967) An electron microscopic autoradiographic study of the site of initial synthesis of RNA in the nucleolus of Smittia. Exp. Cell Res. 48, 276–282.

    Article  CAS  Google Scholar 

  • Jebanesan A., Thatheyus A. J. and Selvanayagam M. (1988) Mosquito control by aquatic hemipterans in river Cooum, Madras. Bicovas 1, 203–211.

    Google Scholar 

  • Jayachandra C. M. and Venkatesan P. (1988) Predatory efficiency of giant water bug, Lethocerus indiens Lep & Serv (Hemiptera:Be-lostomatidae: Lethocerinae). Bicovas 1, 13–26.

    Google Scholar 

  • King R.C. and Burnett R. G. (1959) Autoradiographic studies of uptake of tritiated glycine, thymidine and uridine by fruit fly ovaries. Science 19, 1674–1675.

    Article  Google Scholar 

  • Kumar D. (1978) Histophysiological studies of the salivary apparatus of Indian Hemiptera: Morphological, histological, histochemical, autoradiographic and electron microscopic investigations. Ph.D. thesis, BHU,Varanasi, India.

    Google Scholar 

  • Miles P.W. (1972) The saliva of Hemiptera. Adv. Insect Physiol. 9, 183–225.

    Article  CAS  Google Scholar 

  • Miller N.C.E.(1971) The Biology of the Heteroptera. Second (revised) edition, Hampton (England). E. W. Classey.

    Google Scholar 

  • Muhlach W. E. and Schwalm F. E. (1977) Utilisation of uridine in developing ovarioles of the kelp fly, Coelopa. J. Insect Physiol. 23, 931–937.

    Article  CAS  Google Scholar 

  • Nummelin M. (1988) Water striders (Het: Gerridae) as predators of hatching mosquitoes. Bicovas 1, 121–125.

    Google Scholar 

  • Ramamurty P.S. (1963) Uberdie Horkunft der Ribonulkeinsaure in den wachsenden Eizellen der Skorpionsfliege, Panorpa communis. Naturwischft 50, 383–384.

    Article  Google Scholar 

  • Ray A. (1979) Autoradiographic studies on RNA synthesis in trophophocytes and germinal vesicles of Menochilus sexmaculatus (Coleoptera: Coccinellidae). Entomon 4, 1–5.

    CAS  Google Scholar 

  • Ray A. and Ramamurty P. S. (1979) Sources of RNA supply to the oocytes in Crynodes peregrinus F. (Coleoptera: Chrysomelidae) Int. J. Insect Morphol. Embryol. 8, 113–122.

    Article  Google Scholar 

  • Sirlin J. L. and Jacob J. (1964) Sequential and reversible inhibition of synthesis of RNA in the nucleolus and chromosomes. Effect of benzamide and substituted benzamiadozoles on dipteran salivary glands. Nature 204, 545–547.

    Article  CAS  Google Scholar 

  • Takeda N. (1976) Activatory mechanisms of the prothoracic glands of Monema flavescens (Lepidoptera) with special reference to the secretion of ecdysone. Biol. Bull. 150, 500–521.

    Article  CAS  Google Scholar 

  • Wiley M. J. and Lai-Fook J. (1974) Studies on the silk glands of Calpodesethlius Stall. (Lepidoptera:Hersperudae). J. Morphol. 144, 297–322.

    Article  CAS  Google Scholar 

  • Yadav P. R. (1988) Studies on the salivary apparatus and saliva of some Hemiptera. Ph.D. thesis, BHU,Varanasi, India.

    Google Scholar 

  • Yadav P.R. (1990) Morphological, histological and histochemical observation on the salivary glands of Ranatrafiliformis Fabr (Heteroptera). J. Zool. Res. 3, 89–99.

    Google Scholar 

  • Yadav P. R. (1992) Protein synthesis in the salivary glands of some bugs: An autoradiographic study of 3H glycine incorporation. Insect Sci. Applic. 13, 49–58.

    CAS  Google Scholar 

  • Zalokar M.(1960) Sites of ribonucleic acid and protein synthesis in Drosophila. Exp.Cell Res. 19, 184–196.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Silkworm Seed Technology Laboratory, Kodathi, Carmelram post, Bangalore, 560035, India

    P. R. Yadav

  2. Department of Zoology, Banaras Hindu University, Varanasi, 221005, India

    Dinesh Kumar

Authors
  1. P. R. Yadav
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dinesh Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yadav, P.R., Kumar, D. Synthesis and Transport of RNA in the Salivary Glands of Some Bugs (Heteroptera). Int J Trop Insect Sci 14, 411–418 (1993). https://doi.org/10.1017/S1742758400014065

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1017/S1742758400014065

Key Words

Mots Clés

Search

Navigation