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Isoenzyme Studies on two Field Populations of Glossina pallidipes Austen (Diptera, Glossinidae) in Kenya

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Abstract

Electrophoretic studies were carried out on isozymes of Glossina pallidipes to determine whether there are any genetic differences between natural populations from an area free of sleeping sickness (Nguruman) and an area where sleeping sickness is endemic (Lambwe Valley) in Kenya. Out of 12 enzymes examined, two enzymes, GPI and PGM, showed high polymorphism in the two populations, while the other 10 enzymes were all monomorphic. At the GPI locus, at least five alleles were detected, and two alleles were found at the PGM locus. There was a difference in the frequency of isozyme patterns between males and females, indicating that the PGM locus is on the sex-linked chromosome (X-chromosome). Nei’s genetic distance (D) was 0.00154 between the two populations, which is the value within the range for local populations in different organisms examined so far. Some difference between the two populations was found in the level of average heterozygosity (H) and in allelic composition of the GPI locus, that is, the Lambwe Valley population is more heterogeneous than the Nguruman population.

Résumé

Plusieurs isozyme de Glossina pallidipes ont èté analysés par électrophorèse pour savoir si des différences génétiques existaient entre les populations d’une région non-endémique (Nguruman) et celles d’une région endèmique (Lambwe Valley). La trypanosomiase humaine est prèsente dans cette région du Kenya. Des 12 enzymes analysés, 2 sont polymorphiques (GPI et PGM) alors que les 10 autres sont monomorphiques. Pour le locus GPI, 5 alleles au moins sont dètectés alors que 2 allèles sont prèsents dans le locus PGM. Une différence dans la fréquence du schéma d’isozyme est trouvée entre maies et femalles indiquant que le locus PGM est lié au chromosome sexuel (chromosome X). Les distances génétiques de Nei (D) sont de 0.00154 entre les populations des régions non-endèmiques et endèmiques. Elles correspondent à la valeur trouvée à l’intèrieuve des populations locales pour lest différents organismes ètudiés jusqui à présent. Quelques différences entre les deux populations ont èté trouvèes pour la moyenne d’hétèrozygotie (H) et dans la composition allèlique du locus GPI. La population de Lambwe Valley est plus hètérozygote que celle de Nguruman.

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Authors and Affiliations

  1. Department of Parasitology, Kochi Medical School, Oko, Nankoku, Kochi, 781-51, Japan

    Takeshi Agatsuma

  2. International Centre of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya

    Leonard H. Otieno

Authors
  1. Takeshi Agatsuma
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  2. Leonard H. Otieno
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Agatsuma, T., Otieno, L.H. Isoenzyme Studies on two Field Populations of Glossina pallidipes Austen (Diptera, Glossinidae) in Kenya. Int J Trop Insect Sci 9, 527–530 (1988). https://doi.org/10.1017/S1742758400011085

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  • DOI: https://doi.org/10.1017/S1742758400011085

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