Abstract
Two monoclonal antibodies (Mabs), that react in an enzyme linked immunoabsorbant assay, were generated against Aedes aegypti vitellin. One of these Mabs, VIIG9 that reacted predominantly with a 64 kDa component of Ae. aegypti yolk protein, also recognized 68 kDa and 66 kDa components in the yolk proteins of Culex sitiens and Cx. annulirostris, respectively. Mab VIIG9 has been used to detect vitellin or vitellin precursors in gravid female and non-blood fed nulliparous female mosquitoes as a possible age grading method. The presence of polyclonal and monoclonal antibodies to vitellin in the blood meal did not affect the fecundity of Ae. aegypti.
Résumé
Deux anticorps monoclones (Mabs), qui reagissent dans un essai enzyme lie et immunoabsorbant, ont ete generes contre Aedes aegypti vitelline. L’un de ces Mabs, V11G9 qui a reagi d’une maniere predominante avec un constituant 64 kDa de proteine de vitellus de Ae. aegypti a egalement reconnu les constituants 68kDa et 66 kDa dans les proteines de vitellus de Culex sitiens et de Cx. annulirostris respectivement. On s’est servi de Mab VIIG9 pour decouvrir la vitelline ou les precurseurs de la vitelline dans des femelles pleines et des moustiques femelles non nourries de sang et nullipares comme une methode possible de classer l’age. La presence d’anticorps polyclones et monoclones a la vitelline dans le sang absorbe n’a pas eu d’effect sur la fecondite de Ae. aegypti.
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Ramasamy, M.S., Sands, M., Gale, J. et al. Mosquito Vitellin: Structural and Functional Studies with Monoclonal Antibodies. Int J Trop Insect Sci 9, 499–504 (1988). https://doi.org/10.1017/S1742758400011036
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DOI: https://doi.org/10.1017/S1742758400011036