Skip to main content
Log in

Immunochemical Ablation of Accessory Reproductive Glands of the Male Desert Locust

  • Research Article
  • Published:
International Journal of Tropical Insect Science Aims and scope Submit manuscript

Abstract

Injection of antiserum prepared against accessory reproductive gland (ARG) secretions of the male desert locust, Schistocerca gregaria, disrupted the normal growth and function of the ARG complex in sexually maturing adults. Male locusts were injected with either antiserum or normal guinea-pig serum on days 3, 7 and 10 after adult ecdysis. When the injection of ARG antiserum began as early as day 3 of adult development a marked inhibition of ARG growth was accompanied by the suppression of other characteristics of sexual maturation (yellow body coloration and mating behaviour). Inhibition of ARG growth, while significant, was less pronounced when antiserum injections began on day 7 of adult development, and the injected males did not exhibit normal mating behaviour when placed with virgin females. When single antiserum injections were administered on day 10 of adult development, the males still attained their yellow body coloration by day 19 and copulated normally with virgin females, although their ARGs were only equivalent in size to those 13-day-old males.

Résumé

L’injection de l’antiserum préparé contre les sécretions de la glande reproductrice auxilliair (ARG) des locustes males du desert, Schistocerca gregaria, interompt la eroissance normale et le fouctionnement du complexe ARG chezles adultes sexuellement matures. Les locustes mâles sont injectes auee aussi bien l’antiserum qu’avec le serum normal de cobaye aux jours 3, 7 et 10 aprés la transformation adulte. Quand l’injection de l’antiserum ARG commence tres tôt au jour 3 du developpment adulte, une inhibition, marquée de la croissance de l’ARG est accompagnée par la suppression des autres caracteristiques de la maturation sexuelle (une coloration jamme du corps, et un comportement sexuel). L’inhibition de la croissance de l’ARG quoique significative, est moius pronnoucée quand l’injection de l’antiserum de’bute au jour 7 du du developpment adulte et les maies injecte’s néxhibeut pas de compartement sexuel normal quand ils sout placés en presence de females. Quand des injections uniques de l’antiserum sout adminstrées au jour 10 du developpment adulte, les maies gardent eucore leur coloration corporelle jaune jusqu’au jour 19. Et s’accouplent normalement avec les females vierges bien que leurs ARGs sont seulement equivalents en grandeur a’ ceux des males ages de 13 jours.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alger E. N. and Cabrera E. J. (1972) An increase in death rate of Anopheles stephensi fed on rabbits immunized with mosquito antigen. J. econ. Ent. 65, 165–168.

    Article  CAS  Google Scholar 

  • Allen J. R. and Humphreys S. J. (1979) (Immunization of guinea pigs, and cattle against ticks. Nature 280, 491–493.

    Article  CAS  Google Scholar 

  • Bun Ng T., Campbell C. M. and Idler D. R. (1980) Antibody inhibition of vittellogenesis and oocyte maturation in salmon and flounder. Gen. comp. Endocr. 41, 233–239.

    Article  Google Scholar 

  • Gregory G. E. (1965) The formation and fate of the spermatophore in the African migratory locust, Locusta migratoria migratorioides Reiche and Fairmaire. Trans. R. ent. Soc. Lond. 117, 33–66.

    Article  Google Scholar 

  • Hagedorn H. H. and Kunkel J. G. (1979) Vitellogenin and vitellin in insects. A. Rev. Ent. 24, 475–505.

    Article  CAS  Google Scholar 

  • Hartmann R. (1970) Experimentelle und histologische Untersuchungen der spermatophorenbildung beki der Feldheuschrecke Gomphocerus rufus L. (Ortheptera;Acrididae). Z. Morph. Tiere. 68, 140–176.

    Article  Google Scholar 

  • Hunter-Jones P. (1966) Rearing and Breeding Locusts in the Laboratory. Anti-Locust Research Centre, London.

    Google Scholar 

  • Kaaya G. P. and Alemu P. (1982) Fecundity and survival of tsetse maintained on immunized rabbits. Insect Sci. Applic. 3, 237–241.

    Google Scholar 

  • Loher W. (1961) The chemical acceleration of the maturation process and its hormonal control in the male of the desert locust. Proc. R. Soc. B 153, 380–397.

    Google Scholar 

  • Lowry O. H., Rosebrough N. J., Farr A. L. and Randall R. J. (1951) Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275.

    CAS  PubMed  Google Scholar 

  • Norris M. J. (1954) Sexual maturation in the desert locust (Schistocerca gregaria Forskal) with special reference to the effects of grouping. Anti-Locusts Bull. 18, 1–44.

    Google Scholar 

  • Odhiambo T. R. (1966) Growth and hormonal control of sexual maturation in the male desert locust, Schistocerca gregaria Forskal. Trans. R. ent. Soc. Lond. 118, 393–412.

    Article  Google Scholar 

  • Odhiambo T. R. (1969) The architecture of the accessory reproductive glands of the male desert locust. I. Types of glands and their secretions. Tissue Cell 1, 155–182.

    Article  CAS  Google Scholar 

  • Pener M. P. (1965) On the influence of corpora allata on maturation and sexual behaviour of Schistocerca gragaria. J. Zool. 147, 119–136.

    Google Scholar 

  • Pener M. P. (1967) Effects of allatectomy and sectioning of the nerves of the corpora allata on oocyte growth, male sexual behaviour and colour change in adults of Schistocerca gregaria. J. Insect Physiol. 13, 665–684.

    Article  Google Scholar 

  • Pickford R. and Padgham D. (1973) Spermatophore formation and spermtransfer in the desert locust, Schistocerca gregaria (Orthoptera; Acrididae). Can. Ent. 105, 613–719.

    Article  Google Scholar 

  • Rembold H., Eder J. and Ulrich G. M. (1980) Inhibition of allatotropic activity and ovary development in Locusta migratoria by anti-brain-antibodies. Z. Naturforsch. 35c, 1117–1119.

    Article  Google Scholar 

  • Scheidegger J. J. (1955) Une micro-methode de l’immuno-electrophrese. Inter. Arch. Allergy Appl. Immun. 7, 103–110.

    Article  CAS  Google Scholar 

  • Schiein Y. and Lewis C. T. (1976) Lesions in hematophagus flies after feeding on rabbits immunized with fly tissues. Physiol. Ent. 1, 55–59.

    Article  Google Scholar 

  • Shirk P. D., Dahm K. H. and Roller H. (1976) The accessory sex glands as repository for juvenile hormone in male cecropia moths. Z. Naturforsch. 31c, 199–200.

    Article  CAS  Google Scholar 

  • Shivers C. A., Dudkiewicz A. B., Franklin L. E. and Fussell E. N. (1972) Inhibition of sperm-egg interaction by specific antibody. Science 178, 1211–1213.

    Article  CAS  Google Scholar 

  • Sutherland G. B. and Ewen A. B. (1973) Fecundity decrease in mosquitoes ingesting blood from specifically sensitized mammals. J. Insect Physiol. 20, 655–660.

    Article  Google Scholar 

  • Wikel S. K. (1982) Immune responses to arthropods and their products. A. Rev. Ent. 27, 21–48.

    Article  CAS  Google Scholar 

  • Wikel S. K. (1984) Immunomodulation of host responses to ectoparasite infestation—an overview. Vet. Parasit. 14, 321–339.

    Article  CAS  Google Scholar 

  • Willadsen P. (1980) Immunity to ticks. Adv. Parasit. 18, 293–313.

    Article  CAS  Google Scholar 

  • Wyatt G. R. and Pan M.-L. (1978) Insect plasma proteins. A. Rev. Biochem. 47, 779–817.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dhadialla, T.S., Odhiambo, T.R. & Wagner, G.G. Immunochemical Ablation of Accessory Reproductive Glands of the Male Desert Locust. Int J Trop Insect Sci 7, 465–470 (1986). https://doi.org/10.1017/S174275840000967X

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1017/S174275840000967X

Key Words

Mots Cléf

Navigation