Abstract
Electrophoretic variation was determined at eight enzyme loci in Anopheles arabiensis from four villages in Mwea Irrigation Scheme, Kenya. Seven loci had polymorphic alleles of which at least two were common. Pooled allele frequencies at the Pgm, ß-Had and Odh loci were in Hardy-Weinberg equilibrium, while those at the Ao, Idh, Adh, Est-1 and 6-Pgd loci showed significant deviations. The frequency of heterozygotes at the Adh, Est-1 and 6-Pgd loci was less than expected, while at the Ao locus, there was an excess. A 2 x 4 contingency χ2-test for each of the loci not in Hardy-Weinberg equilibrium indicated an association between the respective gene frequencies and the villages. The suggested explanation for these observations is that each village constitutes a distinct population, and that the pooled data introduced the Wahlund effect. Allele frequencies at individual loci per village population are most likely at Hardy-Weinberg equilibrium. Larger samples per village need to be examined to facilitate a goodness of fit χ2-test between observed and expected frequencies.
Résumé
La variation électrophorétique était déterminée pour huit bandes enzymatiques dans Anophèles arabiensis provenant de quatre villages de la région irriguée de Mwea au Kenya. Sept bandes avaient des allèles polymorphiques dont au moins deux étaient communs. Les fréquences ressemblées des allèles pour les bandes Pgm, ß-Had et Odh étaient en équilibre de Hardy-Weinberg, alors que celles de Ao, Idh, Adh, Est-1 et 6-Pgd montraient des déviations significatives. La fréquence des hétérozygotes pour les bandes de Adh, Est-1 et 6-Pgd était la moins attendue alors que pour la bande Ao, il y avait un excès. Une contingeance 2 x 4 du test χ2 pour chaque bande non en équilibre de Hardy-Weinberg indiquait une association entre les fréquences respectives de genes et les villages. L’explication suggérée pour ces observations est que chaque village constitue une population distincte et que les données rassemblées introduisaient l’effet Wahlund. Les fréquences des allèles á une bande individuelle par population de village étaient plus vraisemblablement en équilibre de Hardy-Weinberg. Un plus grand nombre d’échantillons par village est nécessaire pour étre en accord avec le test χ2 entre les fréquences observées et celles attendues.
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References
Ayala F. F. and Powell J. R. (1972) Allozymes as characters of sibling species of Drosophila. Proc. Nail. Acad. Sci., U.S.A. 69, 1094–1096.
Ayala F. J., Powell J. R., Tracey M. L., Mourao C. C. and Peres-Salas S. (1972) Enzyme variability in Drosophila willistoni group. 4, Genie variation in natural populations of Drosophila willistoni. Genetics 70, 113–139.
Berlocher, S. H. (1980) An electrophoretic key for distinguishing species of the genus Rhagoletis (Diptera: Tephritidae) as larvae, pupae or adults. Ann. ent. Soc. Am. 73, 131–139.
Berlocher, S. H. (1984) Insect molecular systematics. A. Rev. Ent. 29, 403–433.
Bullini, L. and Coluzzi, M. (1978) Applied and Theoretical significance of electrophoretic studies in mosquitoes (Diptera: Culicidae). Parassitologia, Rome 20, 7–21.
Coluzzi, M. and Bullini, L. (1971) Enzyme variants as markers in the study of pre-copuJatory isolating mech-anisms. Nature, Lond. 231, 455–456.
Coluzzi M. and Sabatini A. (1967) Cytogenetic observations on species A and B of the Anopheles gambiae complex. Parassitologia 9, 73–88.
Coluzzi M. and Sabatini A. (1968) Cytogenetic observations on species C of the Anopheles gambiae complex. Para-ssitologia 10, 155–156.
Coluzzi M. and Sabatini A. (1969) Cytogenetic observations on the saltwater species Anopheles merus and Anopheles melas of the gambiae complex. Parassitologia 11, 177–187.
Coluzzi M., Sabatini A., Petrarca V. and Di Deco M. A. (1979) Chromosomal differentiation and adaptation to human environments in the Anopheles gambiae complex. Trans. R. Soc. Trop. Med. Hyg. 73, 483–497.
Corsaro B. B. and Munstermann C. E. (1984) Identification by electrophoresis of Culex adults. (Diptera: Culicidae) in light-trap samples. J. med. Eni. 21, 648–655.
Davidson G. (1964) The five mating-types in the Anopheles gambiae complex. Riv. Malar. 43, 167–170.
Di Deco M. A., Cancrini G., Coluzzi M., Bullini A. P. B., Cianchi R. and Bullini L. (1978) Linkage studies between chromosome inversions and enzyme loci in the mosquito Anopheles stephensi. The J. Hered. 40, 457–458.
Green C. A. (1972) Cytological maps for the practical identification of females of the three freshwater species of the Anopheles gambiae complex. Ann. Trop. Med. Parasit. 66, 143–147.
Haridi A. M. (1974) Linkage studies on DDT and dieldrin resistance in species A and species B of the Anopheles gambiae complex. Bull. Wld. Hlth. Org. 50, 441–448.
Highton R. B., Bryan J. H., Boreham P. F. L. and Chandler J. A. (1979) Studies on the sibling species Anopheles gambiae Giles an L.Anopheles arabiensis Patton (Diptera: Culicidae) in the Kisumu area, Kenya. Bull. ent. Res. 69, 43–53.
Iqbal M. P., Sakai R. R. and Baker R. H. (1973) The genetics of an alcohol dehydrogenase in the mosquito Anopheles stephensi. J. med. Ent. 10, 309–311.
Lewontin R. R. and Hubby J. L. (1966) A molecular approach to the study of genie hetorozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseu-doobscura. Genetics 54, 595–609.
Mahon R. J., Green C. C. and Hunt R. H. (1976) Diagnostic allozymes for routine identification of adults of Anopheles gambiae complex (Diptera: Culicidae). Bull. ent. Res. 66, 25–31.
May B., Bauer R. L., Vadas R. R. and Granett J. (1977) Biochemical genetic variation in the Family Simuliidae: Electrophoretic identification of the human biter in the isomorphic Simulium jenningsi group. Ann. ent. Soc. Am. 70, 637–640.
Miles S. J. (1976) Taxonomic significance of assortative mating in a mixed field population of Culex pipiens australicus, C.P. quinquefasciatus and C. globocoxitus. Syst. Ent. 1, 263–270.
Miles S. J. (1978) Enzyme variation in the Anopheles gambiae Giles group of species (Diptera: Culicidae). Bull. ent. Res. 68, 85–96.
Miles S. J. (1979) A biochemical key to adult members of the Anopheles gambiae group of species (Diptera: Culicidae). J. med. Eni. 15, 297–299.
Miles S. S. and Paterson H. E. (1979) Protein variation and systematics in the Culex pipiens group of species. Mosq. Syst. 11, 187–202.
Munstermann L. E. (1980) Distinguishing geographic strains of the Aedes atropalpus group (Diptera: Culicidae) by an analysis of enzyme variation. Ann. ent. Soc. Am. 73, 699–704.
Narang S. and Seawright J. A. (1982) Linkage relationships and genetic mapping in Culex and Anopheles. In Proc. Recent Developments in Genetics of Insect Disease Vectors (Edited by Steiner W. W. M., Tabachnick W. J., Rai K. K. and Narang S.) pp. 231–289. Stipes Publ. Co. Champaign, IL.
Narang S. and Seawright J. A. (1983) Genetic and physicochemical studies of ß-hydroxyacid dehydrogenase in An. albimanus. Biochem. Genet. 21, 885–893.
Narang S., Seawright J. J. and Kitzmiller J. B. (1981) Linkage relationships and assignment of esterase-4 and esterase-8 loci to chromosome 3 in Anopheles albimanus. The J. Hered. 72, 157–160.
Narang S., Seawright J. A., Mukiama T. T. and Willis N. L. (1983) Assignment of 6-phosphogluconate dehy-drogenase and glucose oxidase to chromosome 2 of Anopheles albimanus. Can. J. Genet. Cytol. 25, 567–572.
Pashley D. D. and Rai K. S. (1983) Comparison of allozyme and morphological relationships in some Aedes (Stegomyia) mosquitoes (Diptera: Culicidae). Ann. ent. Soc. Am. 76, 388–394.
Saul S. H., Grimstad P. P. and Craig Jr., G. B. (1977) Identification of Culex species by electrophoresis. Amer. J. Trop. Med. Hyg. 26, 1009–1010.
Seawright, J. A. Kaiser, P. E. and Narang, S. (1981) Chromosome manipulation studies of Anopheles albimanus for genetic control. In Cytogenetics and Genetics of Vectors. (Editors Pal, R.; Kitzmiller J. J. and Kanda T.), pp. 249–261. Elsevier Biomedical, NY.
Steiner W. W. M. and Joslyn D. J. (1979) Electrophoresis techniques for the genetic study of mosquitoes. Mosq. News. 39, 35–54.
Wagner R. R. and Selander R. K. (1974) Isozymes in insects and their significance. A. Rev. Ent. 19, 117–138.
White G. B. (1974) Anopheles gambiae complex and disease transmission in Africa. Trans. R. Soc. trop. Med. Hyg. 68, 278–301.
White G. B. (1985) Anopheles gambiae, sp. n., a malaria vector in the Semliki Valley, Uganda, and its relationships with other sibling species of the An. gambiae complex (Diptera: Culicidae). Syst. Ent. 10, 510–522.
White G. B., Magayuka S. S. and Boreham P. F. L. (1972) Comparative studies on sibling species of the Anopheles gambiae Giles complex (Diptera: Culicidae): Bionomics and vectorial activity of species A and species B at Segera, Tanzania. Bull. ent. Res. 62, 295–317.
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Mukiama, T.K. Genetic Variation in Wild Anopheles arabiensis Patton of Mwea Irrigation Scheme, Kenya. Int J Trop Insect Sci 8, 245–249 (1987). https://doi.org/10.1017/S1742758400007293
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DOI: https://doi.org/10.1017/S1742758400007293