Blockage of Ovulation and Insemination in the Tsetse Glossina Morsitans Morsitans with Reserpine

  • M. Samaranayaka-Ramasamy
  • D. L. Denlinger
Research Article


Reserpine (a pharmacological agent of plant origin) injected into females of Glossina Morsitans Morsitans was highly effective in preventing ovulation when injected 1–2 days before the egg is normally ovulated. In contrast, reserpine was only modestly effective in blocking parturition, thus suggesting that these two closely related temporal events may be independently regulated. Males injected with reserpine frequently initiated copulation, but sperm and accessory reproductive gland secretions were never transferred to the female. Reserpine, which presumably acts by depleting stores of amines in the nervous system, may have considerable utility in probing the neuroendocrine regulation of tsetse reproduction.

Key Words

Tsetse Glossina ovulation parturition insemination reserpine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Benschoter C. A. (1966) Reserpine as a sterilant for the Mexican fruit fly Anastrepha ludens (Loew) (Diptera: Tephritidae). J. econ. Ent. 59, 333–334.CrossRefGoogle Scholar
  2. Chaudhury M. F. B., Dhadialla T. S. and Kunyiha R. (1980) Evidence of neuroendocrine relationships between mating and ovulation in the tsetse fiy Glossina morsitans morsitans. Insect Sci. Applic. 1, 161–166.Google Scholar
  3. Cymborowski B. and Kryspin I. M. (1975) Effect of reserpine on development and its neuroendocrine regulation of Galleria mellonella. Gen. Pharmac. 6, 209–213.CrossRefGoogle Scholar
  4. Denlinger D. L., Chaudhury M. F. B. and Dhadialla T. S. (1978) Cyclic AMP is a likely mediator of ovulation in the tsetse fly. Experientia 34, 1296–1297.CrossRefGoogle Scholar
  5. Denlinger D. L. and Ma W-C. (1974) Dynamics of the pregnancy cycle in the tsetse Glossina morsitans morsitans. J. Insect Physiol 20, 1015–1026.CrossRefGoogle Scholar
  6. Denlinger D. L., Saini R. K. and Chaudhury M. F. B. (1983) Parturition in the tsetse fly Glossina morsitans: pattern of activity, sound production, and evidence for control by the mother’s brain. J. Insect Physiol. 29, 715–721.CrossRefGoogle Scholar
  7. Ephrussi B. and Beadle G. W. (1936) A technique for transplantation in Drosophila. Am. Nat. 70, 218–225.CrossRefGoogle Scholar
  8. Evans P. D. (1980) Biogenic amines in the insect nervous system. Adv. Insect Physiol 15, 317–473.CrossRefGoogle Scholar
  9. Frontali N. (1968) Histochemical localization of catecholamines in the brain of normal and drug treated cockroaches. J. Insect Physiol. 14, 881–886.CrossRefGoogle Scholar
  10. Gilman A. G., Goodman L. S. and Gilman A. (1980) Goodman and Gilman’s The Pharmacological Basis of Therapeutics, 6th edn. Macmillan, New York.Google Scholar
  11. Glowacka S. K. (1982) Disturbances in the development of egg chambers of Galleria Mellonella L. (Lepidoptera) after reserpine administration. J. Insect Physiol 28, 249–256.CrossRefGoogle Scholar
  12. Glowacka S. K. and Dutkowski A. B. (1979) Effect of reserpine on the larval-pupal moult of the wax moth, Galleria Mellonella L, (Lepidoptera). Experientia 35, 1491–1492.CrossRefGoogle Scholar
  13. Hays S. B. and Amerson G. M. (1967) Reproductive control in the house fly with reserpine. J. econ. Em. 60, 781–783.Google Scholar
  14. Itard J. (1970) L’appareil reproducteur mâle des glossines (Diptera, Muscidae). Les étapes de sa formation chez la pupe. La Spermatogenese. Revue Élev. Méd. vet. Pays trop. 23, 57–81.CrossRefGoogle Scholar
  15. Masner P., Hout L., Corrivault G. W. and Prudhommf L C. (1970) Effect of reserpine on the function of the gonads and its neuroendocrine regulation of tenebrionid beetles. J. Insect Physiol 16, 2327–2344.CrossRefGoogle Scholar
  16. Orchard I., Ruegg R. P. and Davey K. G. (1983) The role of central aminergic neurons in the action of 20-hydroxyecdysone on neurosecretory cells of Rhodnius prolixus. J. Insect Physiol 29, 387–391.CrossRefGoogle Scholar
  17. Robert A., Grillot J. P. and Raabe M. (1981) La lar-viposition chez la Mouche tse-tsè Glossina fuscipes. Recherches preliminares sur son contrôle. Cr. Acad. Sci. Paris 293, 611–618.Google Scholar
  18. Samaranayaka-Ramasamy M. (1981) Influence of feeding and hormonal factors on sexual maturation in male Glossina Morsitans Morsitans Westw. (Diptera: Glossinidae). insect Sci. Applic. 1, 273–280.Google Scholar
  19. Sloley B. D. and Owen M. D. (1982) The effects of reserpine on amine concentrations in the nervous system of the cockroach (Periplaneta americana). Insect Biochem. 12, 469–476.CrossRefGoogle Scholar
  20. Tobe S. S. and Langley P. A. (1978) Reproductive physiology of Glossina. A. Rev. Ent. 23, 283–307.CrossRefGoogle Scholar

Copyright information

© ICIPE 1985

Authors and Affiliations

  • M. Samaranayaka-Ramasamy
    • 1
  • D. L. Denlinger
    • 1
  1. 1.International Centre of Insect Physiology and EcologyNairobiKenya

Personalised recommendations