Abstract
Isolation of vitellin from mature eggs of the palo verde beetle, Derobracus geminatus, was achieved by a combination of gel permeation and anion-exchange chromatography. It is a phosphoglycolipoprotein (Mr ~250, 000) that contains 8% lipids, 4% carbohydrates and 0.2% protein-bound phosphorus. The vitellin is composed of three apoproteins designated I(Mr ~160, 000), II (Mr ~140, 000) and in (Mr ~50, 000). The carbohydrate moiety is composed exclusively of mannose and N-acetylglucosamine. All three apoproteins are glycosylated as shown by flourescein isothiocyanate-conjugated concanavalin A and periodate-Schiff (PAS) staining suggesting the presence of a high mannose structure. Further evidence for the high mannose structure was obtained by the ability of endo-ß-N-acetylglucosaminidase H treatment to remove the carbohydrate moieties on the apoproteins.
Résumé
L’isolation du vitellus des oeufs murs au Derobracus geminatus fut réalisée grâce à une combination de la filtration à travers un colloïde et à la Chromatographie à échange d’ions. C’est une phospholipoproteine (poids moléculaire 250 000) qui contient 8% de lipides, 4% de glucides et 0.2 % de protéines dénommées I (poids moleculaie 160 000), II (poids moléculaire 140 000 et m (poids moléculaire 50 000). La fraction de glucides est composée exclusivement de mannose et N- acetylglucosamine. Toutes les 3 apoproteines étaient glycosidees et démontraient la presence de la mannose comme ça était démontré grâce à la fluorescence du isothiocyanate conjugué à la concanavalin. A et à la coloration au Periodate de Schiff. Une autre evidence pour démontrer l’existence de la mannose fut obtenue grâce à la l’abilité du endo-ß-N-acetylglucosaminidase H de detacher les fractions de glucides des apoproteines.
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Osir, E.O., Law, J.H. Purification and Characterization of Vitellin from the Palo Verde Beetle, Derobracus Geminatus. Int J Trop Insect Sci 10, 259–267 (1989). https://doi.org/10.1017/S1742758400003489
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DOI: https://doi.org/10.1017/S1742758400003489