Abstract
Polyacrylamide gel disc electrophoresis of the salivary secretion from the tsetse, Glossina morsitans morsitans Westwood, showed 11 protein bands, four of which were characterized as glycoproteins. Sixteen amino acids and four sugars in the hydrolysates of saliva were identified. Thin layer chromatography showed five lipid classes and three phospholipids. The secretion is a clear, transparent, viscous fluid with a pH between 7.00 and 7.5. These findings are discussed in relation to the nutritional requirements of the metacyclic forms of trypanosomes that may be found in the salivary glands.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baptis T. B. A. (1941) The morphology and physiology of the salivary glands of Hemiptera-Hepteroptera. Q. Jl microsc. Sci. 83, 311–319.
Burtt E. (1946) Salivation of Glossina morsitans on the glass slides: a technique for isolating flies. Ann. trop. Med. Parasit. 40, 141.
Chayen J., Bitenwesky L. and Butcher R. G. (1973) Practical Histochemistry. John Wiley, London.
Cross G. A. M. (1975) Identification, purification and properties of clone-specific glycoprotein antigens constituting the surface coat of T. brucei. Parasitology 71, 389–418.
Davies B. J. (1964) Disc electrophoresis—II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121, 404–427.
D’Costa M. A. and Rice M. J. (1973) Localization and quantification of glycogen in the tsetse-fly Glossina morsitans and the blow-fly Chrysoma putoria. Comp. Biochem. Physiol. 45B, 483–485.
Diezel W., Kipperschilärger G. and Hoffman E. (1972) An improved procedure for protein staining in Polyacrylamide gels with a new type of Coomassie Brilliant Blue. Analyt. Biochem. 48, 617–620.
Fairbairn H. and Williamson J. (1956) The composition of tsetse fly saliva—I. A histochemical analysis. Ann. trop. Med. Parasit. 50, 322–333.
Maurer H. R. (1971) Disc Electrophoresis and Related Techniques of Polyacrylamide Gel Electrophoresis. Walter de Gruyter, Berlin.
Olembo N. K. (1972) M.Sc. thesis, University of Nairobi.
Partridge S. M. (1948) Filter paper partition chromatography of sugars. 2: an examination of the blood group A specific substances from hog gastric mucin, and the specific polysaccharide of Bacterium dysenteriae (Shiga). Biochem. J. 42, 251–253.
Pearse A. G. E. (1961) Histochemistry: Theoretical and Applied. J. and A. Churchill, London.
Smith I. (1960) Chromatographic and Electrophoretic Techniques. Interscience, New York.
Toledo F. S. A. and De Magalhaes L. E. (1973) Electroferese em gel amido netodoes para a detecao de isoenzimas en dipteros. Ciênc. Cult. S Paulo 25, 1148–1153.
Trevelyan W. E., Proctor D. P. and Harrison J. S. (1950) Detection of sugars on paper chromatograms. Nature 116, 144–145.
Whitemore E. and Gilbert L. I. (1974) Haemolymph proteins and lipo-proteins in Lepidoptera. A comparative electrophoretic study. Comp. Biochem. Physiol. 47B, 63.
Wigglesworth V. B. (1929) Digestion in tsetse fly. A study of structure and function. Parasitology 21, 288–321.
Wigglesworth V. B. (1931) Digestion in Chrysops silacae Aust. (Diptera, Tabanidae). Parasitology 23, 83–76.
Williamson J. (1956) The composition of tsetse-fly saliva—II. Analysis of amino acids and sugars by paper partition chromatography. Ann. trop. Med. Parasit. 50, 334–344.
Youdeowei A. (1975) A simple technique for observing and collecting the saliva of tsetse flies (Diptera, Glossinidae). Bull. ent. Res. 65, 65–67.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Patel, N.Y., Youdeowei, A. & Odhiambo, T.R. The Composition of the Salivary Gland Secretion of the Tsetse, Glossina Morsitans Morsitans Westwood 1850 (Diptera: Glossinidae). Int J Trop Insect Sci 1, 383–387 (1981). https://doi.org/10.1017/S1742758400000722
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1017/S1742758400000722