Dynamics of the invasive Bemisia tabaci (Homoptera: Aleyrodidae) Mediterranean (MED) species in two West African countries

  • Olivier Gnankine
  • Guillaume Ketoh
  • Thibaud Martin
Research Paper


Bemisia tabaci Gennadius is a major pest on cotton and vegetable plants in Africa. It is considered as a cryptic species complex. Identification of the most damaging species such as the Middle East–Asia Minor 1 (B biotype) and Mediterranean (MED) (which contains the Q and Africa silverleaf (ASL) biotypes) species represents an important step towards the management of B. tabaci. Some data on the geographical distribution of the B. tabaci species complex exist in Burkina Faso, Benin and Togo, but data on the pest’s invasion and displacement dynamics, in relation to time, are lacking. Here, molecular markers (mitochondrial cytochrome oxidase, mtCO1) were used to determine the identity of B. tabaci. Our results illustrate population dynamics on cotton and vegetable plants between 2007, 2009 and 2010. On cotton in southern Togo, ASL was predominant and found in sympatry with Q1. Its frequency decreased slightly over time, i.e. from 92% in 2009 to 90% in 2010. In Burkina Faso, Q1, Q3 and ASL biotypes showed different temporal and spatial distribution patterns. There, Q1 dominated on cotton plants throughout the study. This work provides relevant information about the population dynamics of B. tabaci MED species in two West African countries, Burkina Faso and Togo, in connection with pest management programmes.

Key words

Bemisia tabaci Mediterranean species Q populations ASL populations pest management programmes 


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  1. Ahouangninou, C., Fayomi, B. E. and Martin, T. (2011) évaluation des risques sanitaires et environnementaux des pratiques phytosanitaires des producteurs maraîchers dans la commune rurale de Tori-Bossito (Sud-Bénin). Cahiers Agricultures 20, 216–222.Google Scholar
  2. Alon, M., Alon, F., Nauen, R. and Morin, S. (2008) Organophosphates’ resistance in the B-biotype of Bemisia tabaci (Hemiptera: Aleyrodidae) is associated with a point mutation in an ace1-type acetylcholin-esterase and overexpression of carboxylesterase. Insect Biochemistry and Molecular Biology 38, 940–949.CrossRefGoogle Scholar
  3. Alon, M., Benting, J., Lueke B., Ponge T., Alon F. and Morin, S. (2006) Multiple origins of pyrethroid resistance in sympatric biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae). Insect Biochemistry and Molecular Biology 36, 71–79.CrossRefGoogle Scholar
  4. Boykin, L. M., Shatters, R. G., Rosell, R. C., McKenzie, C. L., Bagnall, R. A., De Barro, P. and Frohlich, D. R. (2007) Global relationships of Bemisia tabaci (Hemiptera: Aleyrodidae) revealed using Bayesian analysis of mitochondrial COI DNA sequences. Molecular Phylogenetics and Evolution 44, 1306–1319.CrossRefGoogle Scholar
  5. Byrne, D. N. (1999) Migration and dispersal by the sweet potato whitefly, Bemisia tabaci. Agricultural and Forest Meteorology 97, 309–316.CrossRefGoogle Scholar
  6. Byrne, D.N. and von Bretzel, R. K. (1987) Similarity in flight activity rhythms in coexisting species of Aleyrodidae, Bemisia tabaci and Trialeurodes abutilonea. Entomologia Experimentalis et Applicata 43, 215–219.CrossRefGoogle Scholar
  7. Byrne, F. J. and Devonshire, A. L. (1997) Kinetics of insensitive acetylcholinesterases in organophosphate-resistant tobacco whitefly, Bemisia tabaci (Gennadius) (Homoptera: Aleyrodidae). Pesticide Biochemistry and Physiology 58, 119–124.CrossRefGoogle Scholar
  8. Byrne, F. J. and Toscano, N. C. (2002) Evaluation of peracid activated organophosphates in studies of insecticide resistance conferred by insensitive acetylcholinester-ases. Journal of Economic Entomology 95, 425–429.CrossRefGoogle Scholar
  9. Chu, D., Wan, F. H., Tao, Y. L., Liu, G. X., Fan, Z. X. and Bi, Y. P. (2008) Genetic differentiation of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) biotype Q based on mitochondrial DNA markers. Insect Science 15, 115–123.CrossRefGoogle Scholar
  10. Crowder, D. W., Ellers-Kirk, C., Yafuso, C., Dennehy, T. J., Degain, B. A., Harpold, V. S., Tabashnik, B. E. and Carrie`re, Y. (2008) Inheritance of resistance to pyriproxyfen in Bemisia tabaci (Hemiptera: Aleyrodi-dae) males and females (B biotype). Journal of Economic Entomology 101, 927–932.CrossRefGoogle Scholar
  11. Crowder, D. W., Horowitz, A. R., De Barro, P. J., Liu Shu S, Showalter, A. M., Kontsedalov, S., Khasdan, V., Shargal, A., Liu, J. and Carrie`re, Y. (2010) Mating behaviour, life history and adaptation to insecticides determine species exclusion between whiteflies. Journal of Animal Ecology 79, 563–570.CrossRefGoogle Scholar
  12. De Barro, P. and Ahmed, M. (2011) Genetic networking of the Bemisia tabaci cryptic species complex reveals pattern of biological invasions. PLoS One 6, e25579.Google Scholar
  13. De Barro, P. J., Liu, S.-S., Boykin, L. M. and Dinsdale, B. (2011) Bemisia tabaci: a statement of species status. Annual Review of Entomology 56, 1–19.CrossRefGoogle Scholar
  14. Delatte, H., Reynaud, B. and Granier, M. (2005) A new silverleaf-inducing biotype Ms of Bemisia tabaci (Hemiptera: Aleyrodidarum) indigenous to the islands of the south-west Indian ocean. Bulletin of Entomological Research 95, 29–35.CrossRefGoogle Scholar
  15. Dennehy, T. J., DeGain, B. A., Harpold, V. S., Brink, S. A., Nichols, R. A. (2004) Whitefly resistance to insecticides in Arizona: 2002 and 2003 results, pp. 1926–1938. In Proceedings of the Beltwide Cotton Conference, 5–9 January 2004, San Antonio, TX, USA. National Cotton Council, Memphis, Tennessee.Google Scholar
  16. Dinsdale, A., Cook, L., Riginos, C., Buckley, Y. M. and De Barro, P. (2010) Refined global analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aleyrodoidae: Aleyrodidae) mitochondrial cytochrome oxidase 1 to identify species level genetic boundaries. Annals of the Entomological Society of America 103, 196–208.CrossRefGoogle Scholar
  17. Dittrich, V., Ernst, G. H., Ruesch, O. and Uk, S. (1990) Resistance mechanisms in sweet-potato whitefly (Homoptera: Aleyrodidae) populations from Sudan, Turkey, Guatemala and Nicaragua. Journal of Economic Entomology 83, 1665–1670.CrossRefGoogle Scholar
  18. Frohlich, D.R., Torres-Jerez, I.I., Bedford, I.D., Markham, P.G. and Brown, J. K. A. (1999) Phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Molecular Ecology 8, 1683–1691.CrossRefGoogle Scholar
  19. Gnankiné O. (2011) Caracterisation moleculaire des populations de Bemisia tabaci (Homoptera: Aleyro-didae) et diffusion de la résistance aux insecticides en Afrique de l’ouest. Rapport de stage, Nov 2010–Mars 2011, Université Claude Bernard de Lyon 1, UMR-CNRS, 22 pp.Google Scholar
  20. Gnankiné O., Mouton, L., Henri, H., Terraz, G., Houndeté T., Martin, T., Vavre, F. and Fleury, F. (2012) Distribution of the Bemisia tabaci (Homoptera: Aleyrodidae) biotypes and their associated symbiotic bacteria on host plants in West Africa. Insect Conservation and Diversity. doi: 10.1111/j.1752-4598.2012.00206.xGoogle Scholar
  21. Gnankiné O., Mouton, L., Savadogo, A., Martin, T., Sanon, A., Vavre, F., Dabire, K.R. and Fleury, F.(2013) Biotype status and resistance to neonicotinoids and carbosulfan in Bemisia tabaci (Hemiptera: Aleyrodidae) in Burkina Faso, West Africa.Google Scholar
  22. Gnankiné O., Traoré D., Sanon, A. and Ouedraogo, A. P. (2004) Impact des traitements insecticides sur les ennemis naturels de Bemisia tabaci au Burkina Faso. Annales de l’Université de Ouagadougou 2, 225–242.Google Scholar
  23. Gueguen, G., Vavre, F., Gnankine, O., Peterschmitt, M., Charif, D., Chiel, E., Gottlieb, Y., Ghanim, M., Zchori-Fein, E. and Fleury, F. (2010) Endosymbiont metacommunities, mtDNA diversity and the evolution of the Bemisia tabaci (Hemiptera: Aleyrodidae) species complex. Molecular Ecology 19, 4365–4378.CrossRefGoogle Scholar
  24. Horowitz, A. R., Kontsedalov, S., Khasdan, V. and Ishaaya, I. (2005) Biotypes B and Q of Bemisia tabaci and their relevance to neonicotinoid and pyriproxyfen resist ance. Archives of Insect Biochemistry and Physiology 58, 216–225.CrossRefGoogle Scholar
  25. Houndété T. A., Kétoh, G. K., Hema, O. S. A., Brévault, T., Glitho, I. A. and Martin, T. (2010) Insecticide resistance in field populations of Bemisia tabaci (Hemiptera: Aleyrodidae) in West Africa. Pest Management Science 66, 1181–1185.CrossRefGoogle Scholar
  26. Hu, J., De Barro, P., Zhao, H., Wang, J., Nardi, F. and Liu, S.-S. (2011) An extensive field survey combined with a phylogenetic analysis reveals rapid and widespread invasion of two alien whiteflies in China. PLoS One 6, e16061. doi:10.1371/journal.pone.0016061Google Scholar
  27. Jones, D. R. (2003) Plant viruses transmitted by whiteflies. European Journal of Plant Pathology 109, 195–219.CrossRefGoogle Scholar
  28. Khasdan, V., Levin, I., Rosner, A., Morin, S., Kontsedalov, S., Maslenin, L. and Horowitz, A. R. (2005) DNA markers for identifying biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) and studying population dynamics. Bulletin of Entomological Research 95, 605–613.CrossRefGoogle Scholar
  29. Liu, S.-S., Colvin, J. and De Barro, P. J. (2012) Species concepts as applied to the whitefly Bemisia tabaci systematics: How many species are there? Journal of Integrative Agriculture 11, 176–186.CrossRefGoogle Scholar
  30. McKenzie, C. L., Hodges, G., Osborne, L. S., Byrne, F. J. and Shatters, R. G. Jr (2009) Distribution of Bemisia tabaci (Hemiptera: Aleyrodidae) biotypes in Florida–investigating the Q invasion. Journal of Economic Entomology 102, 670–676.CrossRefGoogle Scholar
  31. Musa, D. P. and Ren, S. X. (2005) Development and reproduction of Bemisia tabaci (Homoptera: Aleyrodi-dae) on three bean species. Insect Science 12, 25–30.CrossRefGoogle Scholar
  32. Pascual, S. and Callejas, C. (2004) Intra- and interspecific competition between biotypes B and Q of Bemisia tabaci (Hemiptera: Aleyrodidae) from Spain. Bulletin of Entomological Research 94, 369–375.CrossRefGoogle Scholar
  33. Roditakis, E., Grispou, M., Morou, E., Kristoffersen, J., Roditakis, N., Nauen, R., Vontas, J. and Tsagkarakou, A. (2009) Current status of insecticide resistance in Q biotype Bemisia tabaci populations from Crete. Pest Management Science 65, 313–322.CrossRefGoogle Scholar
  34. Roditakis, E., Tsagkarakou, A. and Vontas, J. (2006) Identification of mutations in the para sodium channel of Bemisia tabaci from Crete, associated with resistance to pyrethroids. Pesticide Biochemistry and Physiology 85, 131–166.CrossRefGoogle Scholar
  35. SAS Institute, Inc. (1992–1998) Statview for Windows. Version 5.0. SAS Institute, Cary, North Carolina.Google Scholar
  36. Tsagkarakou, A., Mouton, L., Kristoffersen, J. B., Dokianakis, E., Grispou, M. and Bourtzis, K. (2012) Population genetic structure and secondary endosymbionts of Q Bemisia tabaci (Hemiptera: Aleyrodidae) from Greece. Bulletin of Entomological Research 102, 353–365.CrossRefGoogle Scholar
  37. Tsagkarakou, A., Nikou, D., Roditakis, E., Sharvit, M., Morin, S. and Vontas, J. (2009) Molecular diagnostics for detecting pyrethroid and organophosphate resistance mutations in the Q biotype of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae). Pesticide Biochemistry and Physiology 94, 49–54.CrossRefGoogle Scholar

Copyright information

© ICIPE 2010

Authors and Affiliations

  • Olivier Gnankine
    • 1
  • Guillaume Ketoh
    • 2
  • Thibaud Martin
    • 3
  1. 1.Laboratoire d’Entomologie Fondamentale et Appliquée, 09Université de Ouagadougou, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR-SVT)Ouagadougou 09Burkina Faso
  2. 2.Faculté des SciencesUniversité de LomeLomeTogo
  3. 3.UR HortsysCIRADMontpellier Cedex 5France

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