International Journal of Tropical Insect Science

, Volume 28, Issue 3, pp 144–150 | Cite as

Chemical composition and insecticidal activity of essential oil from Perovskia abrotanoides (Lamiaceae) against Sitophilus oryzae (Coleoptera: Curculionidae) and Tribolium castaneum (Coleoptera: Tenebrionidae)

  • Farideh Arabi
  • Saeid Moharramipour
  • Fatemeh Sefidkon


Perovskia abrotanoides Karel is a wild growing plant in Iran and has been used in traditional Iranian herbal medicine. The present study was conducted to investigate chemical composition and fumigant toxicity of the essential oil from P. abrotanoides against Sitophilus oryzae (L.) and Tribolium castaneum (Herbst). Dry flowering aerial parts of the plant were subjected to hydrodistillation using a modified Clevenger-type apparatus. The composition of the essential oil was analysed by gas chromatography (GC) and GC mass spectrophotometry. Twenty-four compounds representing 98.8% of total oil were identified. The predominant components in the oil were camphor (28.38%) and 1,8-cineole (23.18%). Fumigant toxicity was tested against 1-to 7-day-old adults of S. oryzae and T. castaneum with five replications at 25 ± 1 °C and 65 ± 5% relative humidity in dark conditions. The mortality was increased with concentrations of 32, 161, 322, 483 and 645 μl/l air and with exposure time from 2 to 15 h. The lowest concentration (32 μl/l air) of the oil induced 100% mortality of S. oryzae and T. castaneum after 15 and 8 h exposure, respectively. The oil at 322 μl/l air caused 100% mortality for S. oryzae and T. castaneum within 13 and 7 h exposure, respectively. At 645 μl/l air, the LT50 values (lethal time for 50% mortality) were 8 and 2.84 h for S. oryzae and T. castaneum, respectively. In the probit analysis, LC50 values (lethal concentration for 50% mortality) showed that T. castaneum (LC50 = 11.39 μl/l) was more susceptible than S. oryzae (LC50 = 18.75 μl/l). The essential oil of P. abrotanoides can play an important role in stored grain protection and reduce the need for the same, and also the risks associated with the use of synthetic insecticides.

Key words

Stored product insects essential oil fumigant toxicity Perovskia abrotanoides Sitophilus oryzae Tribolium castaneum 


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  1. Abbott W. S. 1925 A method for computing the effectiveness of an insecticide. Journal of Economic Entomology 18, 265–267.Google Scholar
  2. Aoyagi Y., Takahashi Y., Satake Y., Takeya K., Aiyama R., Matsuzaki T., Hashimoto S. and Kurihara T. (2006) Cytotoxicity of abietane diterpenoids from Perovskia abrotanoides and of their semisynthetic analogues. Bioorganic & Medicinal Chemistry 14, 5285–5291.CrossRefGoogle Scholar
  3. Bell C. H. and Wilson S. M. (1995) Phosphine tolerance and resistance in Trogoderma granarium Everts (Coleoptera: Dermestidae). Journal of Stored Products Research 31, 199–205.CrossRefGoogle Scholar
  4. Butler J. H. and Rodriguez J. M. (1996) Methyl bromide in the atmosphere, pp. 27–90. In The Methyl Bromide Issue (Edited by C. H. Bell, N. Price and B. Chakrabarti). Wiley, West Sussex, UK.Google Scholar
  5. Coats J. R., Karr L. L. and Drewes C. D. (1991) Toxicity and neurotoxic effects of monoterpenoids in insects and earthworms, pp. 305–316. In Naturally Occurring Pest Bioregulators (Edited by P. A. Hedin). ACS Symposium Series No. 449. American Chemical Society, Washington DC, USA.CrossRefGoogle Scholar
  6. Daglish G. J. and Collins P. J. (1999) Improving the relevance of assays for phosphine resistance, pp. 584–593. In Proceedings of the 7th International Working Conference on Stored Product Protection 14–19 October 1998, Beijing, China (Edited by X. Jin, Q. Liang, Y. S. Liang, X. C. Tan and L. H. Guan). Sichuan Publishing House of Science and Technology, Chengdu, China.Google Scholar
  7. Finney D. J. (1971) Probit Analysis, 3rd edn. Cambridge University Press, UK. 333 pp.Google Scholar
  8. Hasan M., Iqbal R., Ullah I. and Inam U. H. (1978) Antibacterial activity of the essential oils of Perovskia abrotanoides. Islamabad Journal of Science 5, 22–25.Google Scholar
  9. Howe R. W. (1965) Losses caused by insects and mites in stored foods and foodstuffs. Nutritional Abstracts and Review 35, 285–302.Google Scholar
  10. Huang Y., Lam S. L. and Ho S. H. (2000) Bioactivities of essential oil from Elettaria cardamomum (L.) Maton. to Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). Journal of Stored Products Research 36, 107–117.CrossRefGoogle Scholar
  11. Isman M. B. (2000) Plant essential oils for pest and disease management. Crop Protection 19, 603–608.CrossRefGoogle Scholar
  12. Isman M. B. (2006) Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology 51, 45–66.CrossRefGoogle Scholar
  13. Jaafari M. R., Hooshmand S., Samiei A. and Hossainzadeh H. (2007) Evaluation of leishmanicidal effect of Perovskia abrotanoides Karel. root extract by in vitro leishmanicidal assay using promastigotes of Leishmania major. Pharmacology Online 1, 299–303.Google Scholar
  14. Kim S. I., Park C., Ohh M. H., Cho H. C. and Ahn Y. J. (2003) Contact and fumigant activities of aromatic plant extracts and essential oils against Lasioderma serricorne (Coleoptera: Anobiidae). Journal of Stored Products Research 39, 11–19.CrossRefGoogle Scholar
  15. Kubo I., Muroi H. and Kubo A. (1994) Naturally occurring antiacne agents. Journal of Natural Products 57, 9–17.CrossRefGoogle Scholar
  16. Lee S., Peterson C. J. and Coats J. R. (2003) Fumigant toxicity of monoterpenoids to several stored product insects. Journal of Stored Products Research 39, 77–85.CrossRefGoogle Scholar
  17. Lee B. H., Choi W. S., Lee S. E. and Park B. S. (2001a) Fumigant toxicity of essential oils and their constituent compounds towards the rice weevil, Sitophilus oryzae (L.). Crop Protection 20, 317–320.CrossRefGoogle Scholar
  18. Lee S. E., Lee B. H., Choi W. S., Park B. S., Kim J. G. and Campbell B. C. (2001b) Fumigant toxicity of volatile natural products from Korean spices and medicinal plants towards the rice weevil, Sitophilus oryzae (L.). Pest Management Science 57, 548–553.CrossRefGoogle Scholar
  19. Lee B. H., Annis P. C., Tumaalii F. and Choi W. S. (2004) Fumigant toxicity of essential oils from the Myrtaceae family and 1,8-cineole against 3 major stored-grain insects. Journal of Stored Products Research 40, 553–564.CrossRefGoogle Scholar
  20. MBTOC (Methyl Bromide Technical Options Committee), (1993) tiMethyl Bromide Technical Options Committee: Assessment of Alternatives to Methyl Bromide. United Nations Environment Programme, Ozone Secretariat, Nairobi, Kenya. 374 pp.Google Scholar
  21. Morteza-Semnani K. (2004) The essential oil composition of Perovskia abroatanoides from Iran. Pharmaceutical Biology 42, 214–216.CrossRefGoogle Scholar
  22. Nassiri Asl M., Parvardeh S., Niapour M. and Hosseinzadeh H. (2002) Antinociceptive and anti-inflammatory effects of Perovskia abrotanoides aerial part extracts in mice and rats. Journal of Medicinal Plants 3, 25–33.Google Scholar
  23. Negahban M. and Moharramipour S. (2007) Fumigant toxicity of Eucalyptus intertexta, Eucalyptus sargentii and Eucalyptus camaldulensis against stored-product beetles. Journal of Applied Entomology 131, 256–261.CrossRefGoogle Scholar
  24. Negahban M., Moharramipour S. and Sefidkon F. (2006a) Chemical composition and insecticidal activity of Artemisia scoparia essential oil against three coleopteran stored product insects. Journal of Asia-Pacific Entomology 9, 1–8.CrossRefGoogle Scholar
  25. Negahban M., Moharramipour S. and Sefidkon F. (2006b) Insecticidal activity and chemical composition of Artemisia sieberi Besser essential oil from Karaj, Iran. Journal of Asia-Pacific Entomology 9, 61–66.CrossRefGoogle Scholar
  26. Negahban M., Moharramipour S. and Sefidkon F. (2007) Fumigant toxicity of essential oil from Artemisia sieberi Besser against three stored-product insects. Journal of Stored Products Research 43, 123–128.CrossRefGoogle Scholar
  27. Ojimelukwe P. C. and Alder C. (1999) Potential of zimtaldehyde, 4-allyl-anisol, linalool, terpineol and other phytochemicals for the control of confused flour beetle (Tribolium confusum J.D.V.) (Col: Tenebrionidae). Journal of Pesticide Science 72, 81–86.Google Scholar
  28. Papachristos D. P. and Stamopoulos D. C. (2002) Repellent, toxic and reproduction inhibitory effect of essential oil vapours on Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae). Journal of Stored Products Research 38, 117–128.CrossRefGoogle Scholar
  29. Pillmoor J. B., Wright K. and Terry A. S. (1993) Natural products as a source of agrochemicals and leads for chemical synthesis. Journal of Pesticide Science 39, 131–140.CrossRefGoogle Scholar
  30. Prates H. T., Santos J. P, Waquil J. M., Fabris J. D., Oliveira A. B. and Foster J. E. (1998) Insecticidal activity of monoterpenes against Rhyzopertha dominica (F.) and Tribolium castaneum (Herbst). Journal of Stored Products Research 34, 243–249.CrossRefGoogle Scholar
  31. Rechinger K. H. (1982) Flora Iranica. Akademische Druck und Verlagsanstalt. Graz-Austria 150, 47.Google Scholar
  32. Regnault-Roger C., Hamraoui A., Holeman M., Theron E. and Pinel R. (1993) Insecticidal effect of essential oils from Mediterranean plants upon Acanthoscelides obtectus (Say) (Coleoptera: Bruchidae), a pest of kidney bean (Phaseolus vulgaris L.). Journal of Chemical Ecology 14, 1965–1975.Google Scholar
  33. Rustaiyan A., Masoudi S., Ameri N., Samiee K. and Monfared A. (2006) Volatile constituents of Ballota aucheri Boiss., Stachys benthamiana Boiss. and Perovskia abrotanoides Karel. growing wild in Iran. Journal of Essential Oil Research 18(2), 218–221.CrossRefGoogle Scholar
  34. Sahaf B. Z., Moharramipour S. and Meshkatalsadat M. H. (2007) Chemical constituents and fumigant toxicity of essential oil from Carum copticum against two stored product beetles. Insect Science 14, 213–218.CrossRefGoogle Scholar
  35. Sairafianpour M., Christensen J., Staerk D., Budnik B. A., Kharazmi A., Bagherzadeh K. and Jaroszewski J. W. (2001) Leishmanicidal, antiplasmodial and cytotoxic activity of novel diterpenoid 1,2-quinones from Perovskia abrotanoides: new source of tanshiones. Journal of Natural Products 64, 1398–1403.CrossRefGoogle Scholar
  36. Sajjadi S. E., Mehregan I., Khatamsaz M. and Asghari G. H. (2005) Chemical composition of the essential oil of Perovskia abroatanoides Karel growing wild in Iran. Flavor and Fragrance Journal 20, 445–446.CrossRefGoogle Scholar
  37. Shaaya E., Kostjukovski M., Eilberg J. and Sukprakarn C. (1997) Plant oils as fumigants and contact insecticides for the control of stored-product insects. Journal of Stored Products Research 33, 7–15.CrossRefGoogle Scholar
  38. Shaaya E., Ravid U., Paster N., Juven B., Zisman U. and Pisarrev V. (1991) Fumigant toxicity of essential oils against four major stored-product insects. Journal of Chemical Ecology 17, 499–504.CrossRefGoogle Scholar
  39. Shakarami J., Kamali K., Moharramipour S. and Meshkatalsadat M. (2003) Fumigant toxicity and repellency of essential oil of Artemisia aucheri on four species of stored pest. Applied Entomology and Phytopathology 71, 61–75.Google Scholar
  40. Shakarami J., Kamali K., Moharramipour S. and Meshkatalsadat M. (2004) Effects of three plant essential oils on biological activity of Callosobruchus maculatus F. (Coleoptera: Bruchidae). Iranian Journal of Agricultural Science 35, 965–972.Google Scholar
  41. Singh D., Siddiqui S. and Sharma S. (1989) Reproduction retardant and fumigant properties in essential oils against rice weevil (Coleoptera: Curculionidae) in stored wheat. Journal of Economic Entomology 82, 727–733.CrossRefGoogle Scholar
  42. Singh G. and Upadhyay R. K. (1993) Essential oils: a potent source of natural pesticides. Journal of Scientific and Industrial Research 52, 676–683.Google Scholar
  43. Srivastava S., Gupta K. C. and Agrawal A. (1988) Effect of plant products on Callosobruchus chinensis Linn. infestation in red gram. Seed Research 16, 98–101.Google Scholar
  44. Tapondjou A. L., Adler C., Fontem D. A., Bouda H. and Reichmuth C. (2005) Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Products Research 41, 91–102.CrossRefGoogle Scholar
  45. Tripathi A. K., Prajapati V., Verma N., Bahi J. R., Bansal R. P., Khanuja S. P. S. and Kumar S. (2002) Bioactivities of the leaf essential oil of Curcuma longa (Var. Ch-66) on three species of stored-product beetles (Coleoptera). Journal of Economic Entomology 95, 183–189.CrossRefGoogle Scholar
  46. Tunc I., Berger B. M., Erler F. and Dagli F. (2000) Ovicidal activity of essential oils from five plants against two stored-product insects. Journal of Stored Products Research 36, 161–168.CrossRefGoogle Scholar
  47. USDA, (2007) National Genetic Resources Program. Germplasm Resources Information Network (GRIN). National Germplasm Resources Laboratory, Beltsville, Maryland. pl?27388Google Scholar
  48. Weinzierl R. A. (2000) Botanical insecticides, soaps and oils, pp. 101–121. In Biological and Biotechnological Control of Insect Pests (Edited by E. R. Jack). Lewis Publishers, Boca Raton, Florida.Google Scholar

Copyright information

© ICIPE 2008

Authors and Affiliations

  • Farideh Arabi
    • 1
  • Saeid Moharramipour
    • 1
  • Fatemeh Sefidkon
    • 2
  1. 1.Department of Entomology, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Research Institute of Forests and RangelandsTehranIran

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