Advertisement

Resistance of Cry1Ab maize to spotted stemborer Chilo partellus (Lepidoptera: Crambidae) in India

  • Nirmal S. HariEmail author
  • Jawala Jindal
  • Nachhattar S. Malhi
Article

Abstract

Three transgenic maize hybrids, viz. Prabal YG, Hishell YG and Double YG, producing Bacillus thuringiensis (Bt) CrylAb protein (event Mon 810), were evaluated under field and laboratory conditions for their resistance to spotted stemborer Chilo partellus (Swinhoe). The Bt maize hybrids suffered less damage than their non-Bt iso-hybrids with respect to per cent plant infestation (0.0-0.6), leaf injury rating (1.0-1.1) and per cent stem tunnelling. These hybrids also resulted in significantly higher larval mortality (79.4-100.0%) in laboratory bioassays. The toxin protein produced in leaves was equally effective against neonates and 15-day-old larvae. Neonate larvae consumed less leaf area (5.0-13.8%) in case of Bt hybrids than their non-Bt iso-hybrids.

Key words

Chilo partellus CrylAb insect resistance transgenic maize 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andow D. A. (2003) Negative and positive data, statistical power, and confidence intervals. Environmental Biosafety Research 2, 1–6.CrossRefGoogle Scholar
  2. Archer T. L., Patrick C., Schuster G., Cronholm G., Bynum E. D. and Morrison W. P. (2001) Ear and shank damage by corn borers and corn earworms to four events of Bacillus thuringiensis transgenic maize. Crop Protection 20, 139–144.CrossRefGoogle Scholar
  3. Dutton A., Romeis J. and Bigler F. (2005) Effects of Bt maize expressing CrylAb and Bt spray on Spodoptera littoralis. Entomologia Experimentalis et Applicata 114, 161–169.CrossRefGoogle Scholar
  4. Environmental Protection Agency (EPA) (2000) Biopesticides registration action document. Bacillus thuringiensis Plant Incorporated Protectants II. Science Assessment Product Characterization. Available from: https://doi.org/www.epa.gov/scipolysap/2000/October/brad2_scienceassessment.pdfGoogle Scholar
  5. Girijashankar V., Sharma H. C., Sharma K. K., Swathisree V., Prasad L. S., Bhat B. V., Royer M., Secundo B. S., Narasu M. L., Altosaar I. and Seetharama N. (2005) Development of transgenic sorghum for insect resistance against the spotted stem borer (Chilo partellus). Plant Cell Reports 24, 513–522.CrossRefGoogle Scholar
  6. Gomez K. A. and Gomez A. A. (1984) Statistical Procedures for Agricultural Research, 2nd edn. Wiley-Interscience, New York. 653 pp.Google Scholar
  7. He K., Wang Z., Wen L. and Zhou D. (2002) Resistance of Bt maize to the Asian corn borer (Lepidoptera: Pyralidae) in China, pp. 19–25. In Proceedings of the 8th Asian Regional Maize Workshop: New Technologies for the New Millennium, Bangkok, Thailand, 5–8 August (Edited by G. Srinivasan, P. H. Zaidi, B. M. Prasanna, F. Gonzalez and K. Lesnick). Jointly organized by Kasetsart University, Thailand, the Department of Agriculture, Thailand and CIMMYT. CIMMYT, Mexico, DEGoogle Scholar
  8. Hofte H. and Whiteley H. R. (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiological Reviews 53, 242–255.PubMedPubMedCentralGoogle Scholar
  9. Kanta U. and Sajjan S. S. (1992) Sugars as feeding stimulants in artificial diet for the mass rearing of Chilo partellus (Swinhoe). Journal of Insect Science 5, 126–129.Google Scholar
  10. Keller M., Baruch S., Strizhov N., Prudovsky E., Regev A., Koncz C., Schekk J. and Zilberstein A. (1996) Digestion of 8-endotoxin by gut proteases may explain reduced sensitivity of advanced instar of Spodoptera littoralis to Cry 1C. Insect Biochemistry and Molecular Biology 26, 365–373.CrossRefGoogle Scholar
  11. Kumar H. (1997) Resistance in maize to Chilo partellus (Swinhoe) (Lepidoptera: Pyralidae): An overview. Crop Protection 16, 243–250.CrossRefGoogle Scholar
  12. Mugo S., Groote H. D., Bergvinson D., Mulaa M., Songa J. and Gichuki S. (2005) Developing Bt maize for resource poor farmers—recent advances in the IRMA project. African Journal of Biotechnology 4, 1490–1504.Google Scholar
  13. Mugo S., Taracha C., Bergvinson D., Odhiambo B., Songa J., Hoisington D., McLean S., Ngatia I. and Gethi M. (2004) Screening cry proteins produced by Bt maize leaves for activity against Kenyan maize stem borers, pp. 102–105. In Proceedings of the 7th Eastern and Southern Africa Regional Maize Conference: Integrated Approaches to Higher Maize Productivity in the New Millennium, Nairobi, Kenya, 11–15 February 2002 (Edited by D. K. Friesen and A. F. E. Palmer). CIMMYT, Mexico, DFGoogle Scholar
  14. Panwar V. P. S. (2005) Management of maize stalk borer, Chilo partellus (Swinhoe) in maize, pp. 376–395. In Stresses on Maize in the Tropics (Edited by P. H. Zaidi and N. N. Singh). Directorate of Maize Research, New Delhi.Google Scholar
  15. Pathak R. S. (1990) Genetics of sorghum, maize, rice and sugarcane resistance to the cereal stem borer, Chilo spp. Insect Science and Its Application 11, 689–699.Google Scholar
  16. Pathak R. S. and Othieno S. M. (1990) Inheritance of resistance to the spotted stem borer Chilo partellus (Swinhoe) in maize. Maydica 35, 247–252.Google Scholar
  17. Prutz G. and Dettner K. (2005) Effects of transgenic Bacillus thuringiensis -maize on larval food consumption, utilization and growth in the grass moth species Chilo partellus under laboratory conditions (Lepidoptera: Crambidae). Entomologia Generalis 28, 161–172.CrossRefGoogle Scholar
  18. The Review Committee on Genetic Manipulation (RCGM) (1998) Regulatory mechanisms for GMOs and products thereof. Available from: https://doi.org/www.dbtindia.nic.inGoogle Scholar
  19. Sanders P. R., Lee T. C, Groth M. E., Aswood J. D. and Fuchs R. L. (1998) Safety assessment of insect protected corn, pp. 241–256. In Biotechnology and Safety Assessment (Edited by J. A. Thomas), 2nd edn. Taylor and Francis, Routledge.Google Scholar
  20. Sekhon S. S., Dhillon B. S. and Kanta U. (1993) Host plant resistance in maize to insect pests, pp. 79–109. In Advances in Host Plant Resistance to Insects (Edited by G. S. Dhaliwal and V. K. Dilawari). Kalyani Publishers, New Delhi.Google Scholar
  21. Siddiqui K. H., Sarup P., Panwar V. P. S. and Marwaha K. K. (1977) Evolution of base ingredients to formulate artificial diet for the mass rearing of Chilo partellus (Swinhoe). Journal of Entomological Research 1, 117–131.Google Scholar
  22. Singh R., Channappa R. K., Deeba F., Nagaraj N. J., Sukavaneaswaran M. K. and Manjunath T. M. (2005) Tolerance of Bt corn (Mon 810) to maize stem borer Chilo partellus (Lepidoptera: Crambidae). Plant Cell Reports 24, 556–560.CrossRefGoogle Scholar

Copyright information

© ICIPE 2008

Authors and Affiliations

  • Nirmal S. Hari
    • 1
    Email author
  • Jawala Jindal
    • 1
  • Nachhattar S. Malhi
    • 1
  1. 1.Maize Section, Maize Entomology Laboratory, Department of Plant Breeding, Genetics and BiotechnologyPunjab Agricultural UniversityLudhianaIndia

Personalised recommendations