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Inherited Sterility in Different Strains of Ephestia Cautella (Lepidoptera: Pyralidae)

  • Ayad A. Al-Taweel
  • Mohammed S. H. Ahmed
  • Sarab S. Kadhum
  • Asaad A. Hameed
  • Majdolin J. Nasser
Research Article

Abstract

Partial sterility induced by gamma irradiation of Ephestia cautella adult males was studied in five laboratory strains (A, B, C, D and E) which exhibit conspicuous genetic variation in the adult fore wing pigmentation. Four of these strains had been reared in the laboratory for more than 80 generations, while the fifth strain has been reared for only 10 generations. When males irradiated with 0.2 kGy were crossed with untreated females, the per cent egg hatch was reduced significantly as compared to the unirradiated control crosses. Strain C showed the highest reduction in the per cent egg hatch followed by a gradual decrease in radiation sensitivity in strains A, E, D and B, respectively. Fecundity and mating frequency appeared not to be affected but the sex ratios were clearly distorted (about two males to one female) in all strains. F1 progeny of all strains were either sterile when mated together (per cent egg hatch=0.0%), but semi sterile when F1 males were mated to normal females (per cent egg hatch = 0.03–3.95 %). Low fertility was observed when F1 females were mated with normal males (per cent egg hatch = 130–15.04%). The cytogenetical investigations showed spermatogenesis to proceed normally in the F1 males, whose fathers had been irradiated (0.2 kGy). However, the primary spermatocyte cells carried multiple chromosomal translocations which were the main cause of the sterility in the F1 males.

Key Words

Inherited sterility F1-sterility Ephestia cautelici stored product pest gamma radiation 

Résumé

La stérilité partielle a été provoquée par l’irradiation de gamma chez les cing mâles adultes de Ephestia Cautella souches de laboratoire (nommées A, B, C, D, et E). Les adults deces souches exposent une visible pigmentation sur l’aile antérieure commeune variation génétique. Les quatre premeiére souches ont été élevées dans le laboratoire plusque 80 génération, tandisque la cinquième jusquâ 10 génération. Léclosion des aeufs ont été diminuées significativement qundune femelle normal croisée avec un mâle irradié de 0.2 kGy de rayons gamma en corn poraison avec celles non-irradiee. On a constaté que l’effet d’irradiation sur léclosion des oeufs de souche C soit plusque lesautres, mais l’effet a diminue progressivement sur les A, E, D, et B, la proportion de sex on té té dénaturée (2 mâle:l femelle) chertoutes les souches après lirradiation, mais la frequence delé-closion et l’union restaient presque normal. D’autre côté la stérilité était total chez les F1 de toutes souches quand on se croisé entre aux, et entre 0.0–3.95% quand une femelle normal croisée avec une F1 maie irradié. On observé ainssi, une faible fertilité quand les deux F1 normal saccoupler (eclosion de 1.3 a 15.04%).

Finalement, notre etudent cytogénétiques ont montrées quele déroulement delà Spermatogenese était normal chez les mâles des F1 provenant de souches irradiées, mais leur cellule spermatocyte apportent une translocation multiple celle aberration chromosomique aète très prolalement la cause principle delà stérilité chez les maies eu F1.

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References

  1. Ahmed, M.S.H., Al-Hakkak, Z.S., and Al-Saqur, A. M. (1971) Exploratory studies on the possibility of integrated control of the fig moth, Ephestia Cautella (Walk.). Application of Induced Sterility for Control of Lepidoptera Populations (Proc. Panel, Vienna, 1970) IAEA pp. 1–6.Google Scholar
  2. Ahmed, M.S.H., Al-Hakkak, Z. S. and Al-Saqur, A. M. (1972) Inherited sterility in the fig moth Cadra (Ephestia) cautella (Walk.). Int. Conf. Peaceful Uses Atom. Energy (Proc. Conf Geneva, 1971) UN, Vienna 12. pp. 383–389.Google Scholar
  3. Ahmed, M. S. H., Lamooza, S. B., Ouda, N. A. and Al-Hassany, I. A. (1975) Preliminary report on mating studies of three varieties of Cadra (Ephestia) cautella (Walk.). Sterility Principle for Insect Control, (Proc. Symp. Innsbruck, 1974). IAEA, Vienna, pp. 413–421.Google Scholar
  4. Ahmed, M. S.H., Al-Taweel, A. A., Ouda, N. A., Lamooza, S. B. and Al-Hassany, I. A. (1975a) Colour inheritance in the forewings of Ephestia cautella. 1st. Congr. Iraq Atom. Energy Comm. (Proc. Congr. Baghdad, 1975) Baghdad, pp. 89–95.Google Scholar
  5. Ahmed, M. S.H. (1976) General information regarding the status of dry dates disinfestation in Iraq. A report submitted to the joint FAO/IAEA Research Coordination Meeting on Technological and Economical Feasibility of Food Irradiation, Wageningen, The Netherlands.Google Scholar
  6. Ahmed, M. S. H., Kadhum, A. A., Hameed, A. A., Ali, S. R. and Al-Hakkak, Z. S. (1984) Cytoplasmic analysis of Ephestia Cautella adult females collected in different regions of Iraq. J. stored. Prod. Res. 20, 151–152.CrossRefGoogle Scholar
  7. Ahmed, M. S.H. (1988) Cytoplasmic incompatibility for suppression of Ephestia Cautella infestation rate in simulated date stores. Modern Insect Control: Nuclear Techniques and Biotechnology (Proc. Symp. Vienna, 1987). IAEA, Vienna, pp. 102–103.Google Scholar
  8. Amoako-Atta, B. and Partida, G. J. (1976) Sensitivity of almond moth pupae to gamma radiation (Lepidoptera; Pyralidae). J. Kans. entomol. Soc. 49, 133–140.Google Scholar
  9. Amoako-Atta, B., Denell, R. E. and Mills, R. B. (1978) Radiation-induced sterility in Ephestia Cautella (Walker) (Lepidoptera: Pyralidae): Recovery of fertility during five generations after gamma irradiation. J. stored Prod. Res. 14, 181–188.CrossRefGoogle Scholar
  10. Al-Hakkak, Z. S., Ali, S. R. and Ahmed, M. S.H. (1982) Differential sterility induced by gamma radiation in the adult males of six strains of Ephestia cautella. Sterile Insect Technique and Radiation in Insect Control IAEA, Vienna, pp. 434–436.Google Scholar
  11. Al-Taweel, A. A. and Fox D.P. (1983) Germ cell differentiation and kinetics in the testes of Dermestes (Coleoptera). Cytologia 48, 605–620.CrossRefGoogle Scholar
  12. Brower, J. H. (1979) Radiosensitivity of adults of the almond moth. J. econ. entomol. 72, 43–47.CrossRefGoogle Scholar
  13. Brower, J. H. (1980) Inheritance of partial sterility in progeny of irradiated males Ephestia Cautella (Lepidoptera: Pyralidae) and its effect on theoretical population suppression. Can. Entomol. 112, 131–140.CrossRefGoogle Scholar
  14. Carpenter, J. E., Young, J. R., Sparks, A. N., Cromroy, H. L. and Chowdhury, M. A. (1987) Com earworm (Lepidoptera: Noctuidae): Effects of substerilizing doses of radiation and inherited sterility on reproduction. J. econ. entomol. 80, 483–489.CrossRefGoogle Scholar
  15. Duncan, D. B. (1955) Multiple range and multiple F. tests. Biometrics 11, 1–42.CrossRefGoogle Scholar
  16. Hussain, A. A. (1974) Date Palms and Dates with their Pests in Iraq. Baghdad Univ. Press.Google Scholar
  17. Hussain, A. A. (1985) Date Palms and Dates with their Pests in Iraq. Basrah Univ. Press.Google Scholar
  18. Husseiny, M. and Madsen, H. F. (1964) Sterilization of the navel orangeworm, Paramyelais transitella (Walk.) by gamma radiation, (Lepidoptera: Phycitidae). Hilgardia 36, 113–137.CrossRefGoogle Scholar
  19. LaChance L. E. (1984) Genetic methods for the control of Lepidopteran pests: Status and potential. F1Sterility for Control of Lepidoptera Pests (Report Consultants meeting, Vienna, 1984). IAEA, Vienna.Google Scholar
  20. Li, Y. Y., Zhang, H. Q., Lou, H. Z. and Zhao C.D. (1988) The inherited sterility of the corn borer, Ostrinia furnacalis (Guen). Modern Insect Control: Nuclear Techniques and Biotechnology (Proc. Symp. Vienna, 1987) IAEA, Vienna, pp. 403–411.Google Scholar
  21. North, D. T. (1975) Inherited sterility in Lepidoptera. A. Rev. Entomol. 20, 167–182.CrossRefGoogle Scholar
  22. North, D. T. and Holt, G. G. (1968) Genetic and cytogenetic basis of radiation-induced sterility in the adult male cabbage looper, Trichoplusia ni. In Isotopes and radiation in entomology. International Atomic Energy Agency Symposium, Vienna, pp. 233–247.Google Scholar
  23. Proshold, F. I. and Barteil, J. A. (1970) Inherited sterility in progeny of irradiated male tobacco budworms: Effects on reproduction, developmental time and sex ratio. J. econ. Entomol. 63, 280–285.CrossRefGoogle Scholar
  24. Proshold, F. I. and Baiteli, J. A. (1972) Differences in radiosensitivity of two colonies of tobacco budworm, Heliothis virescens. Can. Entomol. 104, 995–1002.CrossRefGoogle Scholar
  25. Proverbs, M. D. (1962) Progress on the use of sexual sterility for control of the codling moth, Carpocapsa pomonella (L.) (Lepidoptera: Olethreutidae). Proc. EntomoL Soc. Ont. 92, 5–11.Google Scholar
  26. Walfenbarger, D. A. and Guerra, A. A. (1971) Response of strains and sexes of the tobacco budworm to gamma radiation. J. econ. Entomol. 644, 1412–1415.CrossRefGoogle Scholar

Copyright information

© ICIPE 1989

Authors and Affiliations

  • Ayad A. Al-Taweel
    • 1
  • Mohammed S. H. Ahmed
    • 1
  • Sarab S. Kadhum
    • 1
  • Asaad A. Hameed
    • 1
  • Majdolin J. Nasser
    • 1
  1. 1.Agriculture and Biology FacultyBaghdadIraq

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