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Host Immunoglobulin G in the Haemolymph of the Brown Ear Tick, Rhipicephalus Appendiculatus (Neumann, 1901)

  • S. K. Mbogo
  • E. O. Osir
  • A. O. Mongi
Tick Immunology

Abstract

Adult female Rhipicephalus appendiculatus ticks were fed on rabbits immunized with either bovine serum albumin, solubilized tick midgut membrane proteins, a 50:50 mixture of bovine serum albumin and solubilized tick midgut membrane proteins or buffer. Haemolymph harvested from these ticks after they had fed to engorgement and sera from the immunized rabbits were assayed for the presence and quantity of both the total rabbit IgG and specific rabbit IgG raised against bovine serum albumin using the enzyme linked immunosorbent assay technique. The results obtained indicated that rabbit IgG was able to cross the midgut barrier of the tick into the haemolymph. Furthermore, the amounts of IgG crossing into the haemolymph were dependent on the antibody titres in the serum. The results also showed that feeding ticks on blood containing anti-tick midgut antibodies increased the passage of IgG into the haemolymph.

Key Words

Enzyme linked immunosorbent assay immunization IgG in tickhaemolymph Rhipicephalus appendiculatus solubilized tick midgut membrane proteins 

Résumé

Des tiques Rhipicephalus appendiculatus femelles ont été nourries sur des lapins immunisés à partir soit d’albumine sérique bovin, soit de proteines solubles issues de la membrane intestinale de tiques, soi d’un mélange à égale des deux précédentes substances ou à partir d’une solution tampon. Il a été procédé a moyen de la technique de l’ELISA au dosage de l’IgG des lapins dans l’hémolymphe des tiques de même qu’à l’estimation de la quantité d’hémoglobine secrétée par les lapins contre les substances à eux injectées. Les résultats obtenus, indiquent que l’IgG des lapins peut traverser la barrière intestinale de la tique et se retrouver dans l’hémolymphe. Ils indiquent aussi que le taux d’IgG traversant la barrière intestinale de la tique dépend du taux d’anticorps sérique. Ces résultats de la même manière indiquent que la prise de nourriture par des tiques sur des animaux immuns contre des proteines de tiques d’origine intestinale endommage les structures intestinales et augmente le passage des anticorps de l’intestine à l’hémolymphe.

Mots Clés

Rhipicephalus appendiculatus immunoglobulines G dans l’hémolymphe de tiques ELISA proteines solubles obtenues à partir de la membrane intestinale de tiques immunisation 

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Copyright information

© ICIPE 1992

Authors and Affiliations

  • S. K. Mbogo
    • 1
  • E. O. Osir
    • 2
  • A. O. Mongi
    • 2
  1. 1.Kenya Agricultural Research InstituteNational Veterinary Research CentreKikuyuKenya
  2. 2.The International Centre of Insect Physiology and Ecology (ICIPE)NairobiKenya

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