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Glossina pallidipes Virus: Its Potential for Use in Biological Control of Tsetse

  • M. O. Odindo
Research Article

Abstract

Laboratory-reared tsetse, Glossina pallidipes were inoculated with the tsetse virus by microinjection into the haemocoel, and feeding through micropipettes. The inoculated tsetse were reared on rabbits for 45 days, and feeding, flight and mating activities recorded. The tsetse were dissected and the conditions of the salivary glands and gonads noted. In males, tsetse were examined under a compound microscope and the presence of spermatozoa noted. F1 pupae were allowed to emerge, dissected, and the salivary glands examined for hypertrophy, and the gonads for sterility.

There was no reduction in the activity of inoculated tsetse. Infection level was 23.5% in the treated adults. All infected ♂♂ were sterile while ♀♀ were fertile. There was no significant difference in the maternal age at larviposition, F1 pupal weight, or incubation period of F1 pupae, between treated and untreated tsetse.

A high proportion of F1 adults (65%) were infected, with category four hypertrophied salivary glands. All males with enlarged glands were sterile. The evidence obtained shows that the tsetse virus may be used in biological control of G. pallidipes.

Key Words

Glossina pallidipes virus sterility male tsetse transovarial transmission biological control 

Mots Cléfs

Glossina pallidipes virus stérilité mouches tsé-tsé mâles transmission ovarienne lutte biologique 

Résumé

Des tsé-tsé Glossina pallidipes élevées au laboratoire ont été inoculées avec le virus de tsé-tsé par micro-injection dans le sang et par alimentation par micropipettes. Les tsé-tsé inoculées furent elevées sur des lapins pendant 45 jours et leurs activités relatives â l’alimentation, au vol et accouplement furent enregistrées. Les tsé-tsé furent disséquées et l’etat des glandes salivaires et gonades relevé. Après leur emergence les chrysalides de la génération F1 etaient dissequées; leurs glandes salivaires et gonades examinées pour hypertrophie et sterilité.

Il n’y avait pas de reduction de l’activité chez les tsé-tsé inoculées. Chez les adultes traités, le niveau d’infection était de 23,5%. Tous les mâles infectés étaient steriles par contre les femelles fertiles. Il n’y a pas eu de difference significative dans l’âge de femelles à la larviposition, le poids des nymphes F1, ou la durée d’incubation des nymphes F1 entre les tsé-tsé traitées celles qui ne l’étaient pas. Une importante proportion des adultes à la génération F1 infectés avaient des glandes salivaires hypertrophiées tombant dans la catégorie 4. Tous les mâles ayant des glandes hypertrophiées étaient steriles. Il a été demontréque le virus de tsé-tsé puisse être utilisé dans la lutte biologique contre G. pallidipes.

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Copyright information

© ICIPE 1988

Authors and Affiliations

  • M. O. Odindo
    • 1
  1. 1.The International Centre of Insect Physiology and EcologyMbitaKenya

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