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Pattern of Protein, Glycogen and Trehalose During Development of Polyphenic Forms in the Aphid Lipaphis erysimi (Kalt.) (Aphididae: Hemiptera)

  • Pushpinder J. Rup
  • Satwinder K. Sohal
Research Article

Abstract

Polyphenism is a common feature in the mustard aphid, Lipaphis erysimi (Kalt.) in tropical countries. The function related changes in basic biochemical pool are reported during development of polyphenic forms. A gradual decrease in the protein content in this aphid was recorded, as the nymphs advanced in their instar number and reached adult stage. However, the protein content was significantly lower in the apterous forms as compared to alate. The number, density and mobility of protein bands varied as the nymphs passed through different instars and the number of bands was more in alate forms. The glycogen and trehalose contents showed a decrease upto the third instar and showed an increase in the fourth instar, but again declined in the adults of both the phases.

Key Words

Lipaphis erysimi (Kalt.) mustard aphid polyphenism development protein glycogen trehalose 

Mots Cléfs

Lipaphis erysimi (Kalt.) puceron de la moutarde polyphenisme developpement proteine glycogène trehalose 

Résumé

Le polyphenisme est un trait commun du puceron de la moutarde, Lipaphis erysimi (Kalt.) dans les pays tropicaux. La fonction rattachée au changement de la mare biochimique de base fut observée durant le developpement des formes polypheniques. Une decroissance graduée de la proteine de ce puceron fut notée chez les nymphes pendant un stage avance dans leur developpement et chez les adultes. La contenance en proteines fut d’une manière significative plus basse chez les formes sans ailes que celles ailées. Le nombre, la densité et mobilité des bandes proteiques variaient selon le stage du developpement des nymphes et le nombre des bands etait plus elèvé chez les formes ailées. La contenance en glycogène et trehalose baissa jusqu’au troisième stage du developpement et augmenta au quatrième mais baissa chez les adultes.

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References

  1. Bawa S. R. Kanwar K. C. and Gupta R. K. (1972) Biochemical characteristic of fertile and sterile Callosobruchus maculatus (Ab.). 14th International Congress of Entomology, Canberra (Australia). 22 August 1972.Google Scholar
  2. Burcombe J. V. and Hollingsworth M. J. (1970) The total nitrogen, protein, amino acid and uric acid content of aging Drosophila. Exp. Gerontol. 5, 247–255.CrossRefGoogle Scholar
  3. Chippendale G. M. (1978) The functions of carbohydrates in insect life processes. In Biochemistry of Insects (Edited by Rockstein M.), pp. 1–55. Academic Press, New York.Google Scholar
  4. Davis B. J. (1964) Disc electrophoresis. II. Methods and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427.CrossRefGoogle Scholar
  5. Dixon A. F. G. and Wratten S. D. (1971) Laboratory studies on aggregation, size and fecundity in the black bean aphid, Aphis fabae Scop. Bull. Ent. Res. 61, 97–111.CrossRefGoogle Scholar
  6. Dubois M., Gilles K. A., Hamilton J. K., Robers P. A. and Smith F. (1956) Calorimetric method for determination of sugars and related compounds. Anal. Chem. 28, 350–356.CrossRefGoogle Scholar
  7. Good C. A., Kramer H. and Somogyi M. (1933) The determination of glycogen. J. Biol. Chem. 100, 488–491.Google Scholar
  8. Hardie J. and Lees A. D. (1985) Endocrine control of polymorphism and polyphenism. In Comprehensive Insect Physiology, Biochemistry and Pharmacology (Edited by Kerkut G. A. and Gilbert L. I.), Vol. 8, pp. 441–490. Pergamon Press.Google Scholar
  9. Kim H. R., Yoe S. M., Yu J. H. and Kim K. M. (1983) Carbohydrates and inorganic ions in haemolymph and fat body during metamorphosis of the silkworm. Bombyx mori L. Korean J. Ent. 13, 55–59.Google Scholar
  10. Lowry O. H., Rosenbrough N. J., Farr A. L. and Rendall R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193, 265–275.Google Scholar
  11. Ludwig D. and Rothstein F. (1949) Changes in the carbohydrate and fat content of the Japanese beetle, Popilla japonica (Newman) during metamorphosis. Physiol. Zool. 11, 89–103.Google Scholar
  12. Montogomery R. (1957) Determination of glycogen. Arch. Biochem. Biophys. 67, 378–386.CrossRefGoogle Scholar
  13. Pappas P. W. and Rodrick G. E. (1971) An electrophoretic study of lactate dehydrogenase isozymes, protein and lipoprotein of Drosophila melanogaster larvae, pupae and adults. Com. Biochem. Physiol. (B) 40, 709–713.Google Scholar
  14. Rao K. D. P. and Agarwal H. C. (1970) Lipids of Tro-goderma granarium Everts—II: Lipids and glycogen content during development. Indian J. Ent. 32, 318–323.Google Scholar
  15. Sharma S. P. and Sharma G. (1979) Age related protein changes in bruchids Zabrotes subfasciatus and Cal-losobruchus maculatus. Indian J. Exp. Biol. 17, 1197–1200.Google Scholar
  16. Tate L. G. and Wimer L. T. (1974) Incorporation of l4C from glucose into CO2, chitin, lipid, protein and soluble carbohydrate during metamorphosis of the blowfly, Phormia regina (Dipt.:Calliphoridae). Insect Biochem. 4, 85–98.CrossRefGoogle Scholar
  17. Utsami K. and Natori S. (1980) Changes in head proteins in Sarcophaga peregrina with age. FEBS Lett. 111, 419–422.CrossRefGoogle Scholar
  18. Weeda E. and Kort C. A. D. De (1979) Fuels for energy metabolism in the Colorado potato beetle, Leptinotarsa decemlineata Say. J. Insect Physiol. 25, 951–955.CrossRefGoogle Scholar
  19. Woodring J. P., Clifford C. W., Roe R. M. and Mercier R. R. (1977) Relation of blood composition to age in larval female house cricket, Acheta domestica. J. Insect Physiol. 23, 559–567.CrossRefGoogle Scholar
  20. Wyatt G. R. and Kalf G. F. (1957) The chemistry of insect haemolymph. II. Trehalose and other carbohydrates. J. Gen. Physiol. 40, 833–847.CrossRefGoogle Scholar

Copyright information

© ICIPE 1988

Authors and Affiliations

  • Pushpinder J. Rup
    • 1
  • Satwinder K. Sohal
    • 1
  1. 1.Insect Physiology Laboratory, Department of BiologyGuru Nanak Dev UniversityAmritsarIndia

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