Investigations on Physiological Components of Atherigona Soccata Larvae and Their Interaction with Sorghum—I. Larval Enzymes

  • Z. T. Dabrowski
  • N. Y. Patel


The assumption was made that for a complete understanding of the feeding behaviour and nutrition of Atherigona soccata larvae on various cultivars of sorghum, it was necessary to investigate the relationship between plant chemicals occurring in resistant sorghum plants and the digestive enzymes of larvae. However, at least an introduction to the anatomical organization of the alimentary canal of larvae is needed to understand the digestion and absorption of food taken from resistant and susceptible sorghum lines.

The present preliminary investigation was undertaken to determine qualitatively some of the enzymes present in the gut and salivary glands of the third instar by locating enzymes histochemically on polyacrylamide gels.

Key Words

Atherigona soccata alimentary canal enzymes resistance salivary glands sorghum shootfly 


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  1. Blum A. (1968) Anatomical phenomena in seedlings of sorghum varieties to the sorghum shootfly (Atherigona varia soccata). Crop. Sci. 8, 388–391.CrossRefGoogle Scholar
  2. Davies B. J. (1964) Disc electrophoresis—II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci. 121, 404–427.CrossRefGoogle Scholar
  3. Diezel W., Kipperschilager G. and Hoffman E. (1972) An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie Brilliant Blue. Analyt. Biochem. 48, 617–620.CrossRefGoogle Scholar
  4. Gabriel O. (1973) Methods of Enzymology (Ed. by Jacoby W. B.), Vol. 22, p. 592. Academic Press, New York.Google Scholar
  5. Jotwani M. G. and Srivastava K. P. (1970) Studies on sorghum lines resistant against shootfly Atherigona varia soccata (Rond.). Indian J. Ent. 32, 1–3.Google Scholar
  6. Lee D. and Ryle A. P. (1967) Pepsinogen D. A fourth proteolyptic zynogen from pig gastric mucosa. Biochem. J. 104, 735.Google Scholar
  7. Ponnaiya B. W. X. (1951) Studies in the genus Sorghum: II. The cause of resistance in sorghum to the insect pest Atherigona soccata M. Madras Univ. J. 21, 203–217.Google Scholar
  8. Tingey N. M. and Singh S. R. (1980) Environmental factors influencing the magnitude and expression of resistance. In Breeding Plants Resistant to Insects (Ed. by Maxwell F. G. and Jennings P. R.), pp. 89–111. John Wiley, New York.Google Scholar
  9. Whitemore R. and Filbert L. I. (1974) Haemolymph proteins and lipoproteins in Lepidoptera. A comparative electrophoretic study. Comp. Biochem. Physiol. 47B, 63.Google Scholar
  10. Woodheads and Bernays S. E. (1978) The chemical basis of resistance of Sorghum bicolor to attack by Locusta migratoria. Entomologia exp. appl. 24, 133–144.Google Scholar

Copyright information

© ICIPE 1981

Authors and Affiliations

  • Z. T. Dabrowski
    • 1
  • N. Y. Patel
    • 1
  1. 1.International Centre of Insect Physiology and Ecology (ICIPE)NairobiKenya

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