Abstract
Feeding experiments were carried out to investigate the digestive fate of transgenic DNA and novel protein in wild boar applying polymerase chain reaction (PCR) and immunodiagnostic techniques. Furthermore, the dispersal of viable maize and rapeseed (endozoochory) was studied. A diet containing conventional rapeseed, and either genetically modified (GM) maize expressing Cry1Ab protein (Bt176) or non-GM isogenic maize was offered. By conventional and quantitative PCR both chloroplast-specific plant DNA (rubisco) and cry1Ab gene fragments were detected only in gastrointestinal content. Using an enzyme-linked immunosorbent assay (ELISA) positive signals of immunoactive Cry1Ab protein were detected in digesta samples. Analysis of endozoochory showed that excreted maize seeds retain their germination capacity only in extremely rare cases and no intact rapeseed was found in faeces. A possible dispersal of viable seeds by wild boars is highly unlikely.
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Alexander, T.W., Reuter, T., Aulrich, K., Sharma, R., Okine, E.K., Dixon, W.T., McAllister, T.A., 2007. A review of the detection and fate of novel plant molecules derived from biotechnology in livestock production. Anim. Feed Sci. Technol. 133, 31–62.
Artois, M., Caron, A., Leighton, F.A., Bunn, C., Vallat, B., 2006. Wildlife and emerging diseases. Rev. Sci. Technol. 25, 897–912.
Chowdhury, E.H., Kuribara, H., Hino, A., Sultana, P., Mikami, O., Shimada, N., Guruge, K.S., Saito, M., Nakajima, Y., 2003. Detection of corn intrinsic and recombinant DNA fragments and Cry1Ab protein in the gastrointestinal contents of pigs fed genetically modified corn Bt11. J. Anim. Sci. 81, 2546–2551.
Dardaillon, M., 1987. Seasonal feeding habits of the wild boar in a mediterranean wetland, the Camargue (southern France). Acta Theriol. 32, 389–401.
DeVries, M., Goggi, A.S., Moore, K.J., 2007. Determining seed performance of frost-damaged maize seed lots. Crop Sci. 47, 2089–2097.
Doerfler, W., 2000. Foreign DNA in Mammalian Systems. Wiley-VCH, Weinheim, New York.
Feichtner, B., 1998. Ursachen der Streckenschwankungen beim Schwarzwild im Saarland. Z. Jagdwiss. 44, 140–150.
Flachowsky, G., Chesson, A., Aulrich, K., 2005. Animal nutrition with feeds from genetically modified plants. Arch. Anim. Nutr. 59, 1–40.
Flachowsky, G., Aulrich, K., Bohme, H., Halle, I., 2007. Studies on feeds from genetically modified plants (GMP) — contributions to nutritional and safety assessment. Anim. Feed Sci. Technol. 133, 2–30.
Fournier-Chambrillon, C., Maillard, D., Fournier, P., 1996. Variabilité du régime alimentaire du sanglier (Sus scrofa L.) dans les garrigues de Montpellier (Hérault). Gibier Faune Sauvage 13, 1457–1476.
Geisser, H., Reyer, H.U., 2005. The influence of food and temperature on population density of wild boar Sus scrofa in the Thurgau (Switzerland). J. Zool. 267, 89–96.
Genov, P., 1981. Significance of natural biocenoses and agrocenoses as the source of food for wild boar (Sus scrofa L.). E. POL. 29, 117–128.
Giménez-Anaya, A., Herrero, J., Rosell, C., Couto, S., Serrano, A., 2008. Food habits of wild boars (Sus scrofa) in a Mediterranean coastal wetland. Wetlands 28, 197–203.
Gortázar, C., Ferroglio, E., Höfle, U., Frölich, K., Vicente, J., 2007. Diseases shared between wildlife and livestock: a European perspective. Eur. J. Wildl. Res. 53, 241–256.
Goulding, M.J., Roper, T.J., Smith, G.C., Baker, S.J., 2003. Presence of free-living wild boar Sus scrofa in southern England. Wildl. Biol. 9, 15–20.
Guertler, P., Lutz, B., Kuehn, R., Meyer, H., Einspanier, R., Killermann, B., Albrecht, C., 2008. Fate of recombinant DNA and Cry1Ab protein after ingestion and dispersal of genetically modified maize in comparison to rapeseed by fallow deer (Dama dama). Eur. J. Wildl. Res. 54, 36–43.
Heinken, T., Hanspach, H., Raudnitschka, D., Schaumann, F., 2002. Dispersal of vascular plants by four species of wild mammals in a deciduous forest in NE Germany. Phytocoenologia 32, 627–643.
Herrero, J., Garcia-Serrano, A., Couto, S., Ortuno, V.M., Garcia-Gonzalez, R., 2006. Diet of wild boar Sus scrofa L. and crop damage in an intensive agroecosystem. Eur. J. Wildl. Res. 52, 245–250.
Hohmann, U., Huckschlag, D., 2005. Investigations on the radio caesium contamination of wild boar (Sus scrofa) meat in Rhineland-Palatinate: a stomach content analysis. Eur. J. Wildl. Res. 51, 263–270.
International Seed Testing Association (ISTA), 2004. International Rules for Seed Testing.
James, C., 2007. Global Status of Commercialized Biotech/GM Crops: 2007. ISAAA-Brief 37.
Jennings, J.C., Kolwyck, D.C., Kays, S.B., Whetsell, A.J., Surber, J.B., Cromwell, G.L., Lirette, R.P., Glenn, K.C., 2003. Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal. J. Anim. Sci. 81, 1447–1455.
Keuling, O., Stier, N., Roth, M., 2007. Annual and seasonal space use of different age classes of female wild boar Sus scrofa L. Eur. J. Wildl. Res.
Lutman, P.J.W., Freeman, S.E., Pekrun, C., 2003. The long-term persistence of seeds of oilseed rape (Brassica napus) in arable fields. J. Agric. Sci. Camb. 141, 231–240.
Lutz, B., Wiedemann, S., Einspanier, R., Mayer, J., Albrecht, C., 2005. Degradation of Cry1Ab protein from genetically modified maize in the bovine gastrointestinal tract. J. Agric. Food Chem. 53, 1453–1456.
Lutz, B., Wiedemann, S., Albrecht, C., 2006. Degradation of transgenic Cry1Ab DNA and protein in Bt-176 maize during the ensiling process. J. Anim. Physiol. Anim. Nutr. (Berlin) 90, 116–123.
Phipps, R.H., Beever, D.E., 2000. New technology. Issues relating to the use of genetically modified crops. J. Anim. Feed Sci. 9, 543–561.
Reuter, T., Aulrich, K., 2003. Investigations on genetically modified maize (Bt-maize) in pig nutrition: fate of feedingested foreign DNA in pig bodies. Eur. Food Res. Technol. 216, 185–192.
Schley, L., Roper, T.J., 2003. Diet of wild boar Sus scrofa in Western Europe, with particular reference to consumption of agricultural crops. Mamm. Rev. 33, 43–56.
Schley, L., Dufrêne, M., Krier, A., Frantz, A., 2008. Patterns of crop damage by wild boar (Sus scrofa) in Luxembourg over a 10-year period. Eur. J. Wildl. Res.
Schmidt, M., Sommer, K., Kriebitzsch, W.U., Ellenberg, H., von Oheimb, G., 2004. Dispersal of vascular plants by game in northern Germany. Part I: roe deer (Capreolus capreolus) and wild boar (Sus scrofa). Eur. J. For. Res. 123, 167–176.
Simpson, V.R., 2002. Wild animals as reservoirs of infectious diseases in the UK. Vet. J. 163, 128–146.
Vassant, J., 1994. L’agrainage dissuasif: résultats d’expériences. Bull. Mens. Off. Natl. Chasse 191 (Numéro spécial), 101–105.
Vassant, J., 1997. Agrainage et gestion des populations de sangliers. Bull. Mens. Off. Natl. Chasse 227, 1–4.
Vassant, J., Jullien, J.M., Brandt, S., 1987. Réduction des dégâts de sangliers sur blé et avoine en été. Etude de l’efficacité de l’épandage de maïs en grain en forêt. Bull. Mens. Off. Natl. Chasse 113, 23–33.
Wiedemann, S., Lutz, B., Kurtz, H., Schwarz, F.J., Albrecht, C., 2006. In situ studies on the time-dependent degradation of recombinant corn DNA and protein in the bovine rumen. J. Anim. Sci. 84, 135–144.
Woltz, J., TeKrony, D.M., Egli, D.B., 2006. Corn seed germination and vigor following freezing during seed development. Crop Sci. 46, 1526–1535.
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Wiedemann, S., Lutz, B., Albrecht, C. et al. Fate of genetically modified maize and conventional rapeseed, and endozoochory in wild boar (Sus scrofa). Mamm Biol 74, 191–197 (2009). https://doi.org/10.1016/j.mambio.2008.07.002
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DOI: https://doi.org/10.1016/j.mambio.2008.07.002