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Changes in Matrix Metalloproteinase 2 Activities in Amniochorions During Premature Rupture of Membranes

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Abstract

Objective

Increased proteolytic activities of matrix metalloproteinases (MMPs) such as MMP-3 and MMP-9 are associated with premature rupture of membranes et term. However, it is unclear whether MMP-2 is involved in the premature rupture of membranes. In this study, to elucidate the role of MMP-2, we evaluated the activity of MMP-2 and also the expression of pro-MMP-2, membrane type 1 (MT1)-MMP and tissue inhibitor of metalloproteinase (TIMP)-1 in premature rupture of membranes.

Methods

Amniochrions were prepared from 29 subjects with no labor (cesarean section; CS, n = 10), labor (normal delivery; ND, n = 10), and labor during premature rupture of membranes (PROM, n = 9). MMP-2 activity was spectrophotometrically assayed by measuring the digestion of an MMP-2-specific substrate. The levels of pro-MMP-2, MT1-MMMP and TIME-1 were determined by Western immunoblotting.

Results

The activity of MMP-2 in PROM was significantly higher than that in CS and ND (P <.05). In addition, the levels of MT1-MMP, an activator of MMP-2, were higher in PROM than in CS and ND. In contrast, the level of TIMP-1, an inhibitor of MMP-2 was substantially lower in PROM than CS and ND. Moreover, the levels of pro-MMP-2 were increased more significantly in PROM and ND than in CS (P <.05).

Conclusion

Our results suggest that the increased expression of pro-MMP-2 and MT1-MMP and decreased expression of TIMP-1 may result in the increased activity of MMP-2, which is involved in the degradation of extracellular matrix (ECM) of fetal membrane, thereby inducing the premature rupture of membranes at term.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology and Host Defense and Biochemical Research, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan

    Atsuyuki Ota MD, Hisashi Yonemoto MD, Akimasa Someya PhD, Shigeru Itoh MD, Katsuyuki Kinoshita MD & Isao Nagaoka MD

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  1. Atsuyuki Ota MD
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  2. Hisashi Yonemoto MD
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  3. Akimasa Someya PhD
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  4. Shigeru Itoh MD
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  5. Katsuyuki Kinoshita MD
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  6. Isao Nagaoka MD
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Correspondence to Atsuyuki Ota MD.

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Ota, A., Yonemoto, H., Someya, A. et al. Changes in Matrix Metalloproteinase 2 Activities in Amniochorions During Premature Rupture of Membranes. Reprod. Sci. 13, 592–597 (2006). https://doi.org/10.1016/j.jsgi.2006.10.001

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