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Combined Thyroidectomy and Renal Denervation Suppress Renin Expression and Secretion in Fetal Sheep

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Abstract

Background and objectives

Activity of the fetal renin-angiotensin system (RAS) is developmentally regulated, increasing in late gestation toward tenn. Thyroid hormone and the renal nerves are both important modulators of renal RAS maturation; however, ablation of either influence alone does not totally block the aforementioned developmental late gestation increase in RAS in fetal sheep. In the current study, we used the technique of thyroidectomy combined with bilaterl renal denervation (TX+D), which removes thyroid hormone from the circulation and abolishes effects of renal nerve activity, to determine if simultaneous removal of their effects on the kidney would markedly alter renin expression and secretion in late gestation.

Methods

TX+D was performed at 120 days of gestation age (dGA). Control fetuses were sham-operated. Immediately before necropsy (∼ 138 dGA), fetuses were infused with isoproterenol to examine plasma acive and prorenin changes in response to beta-adrenergic stimulation.

Results

TX+D decreased plasma thyroid hormone concentrations, renal renin mRNA, renal active and prorenin levels, and plasma active and prorenin concentrations. Isoproterenol-induced increases in plasma active renin were also reduced in TX+D fetuses. TX+D did not alter renal angioensin (Ang) II subtype receptor (AT2) expression close to term.

Conclusion

These findings suggest that TX+D synergize in the suppression of fetal renin expression.

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Authors and Affiliations

  1. Departments of Obstetrics/Gynecology and Physiology/Pharmacology, and the Center for Research in Obstetrics and Gynecology, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157-1066, USA

    Kai Chen MD, MS, Luke C. Carey PhD, Nancy K. Valego PhD, Jingfang Liu PhD & James C. Rose PhD

Authors
  1. Kai Chen MD, MS
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  2. Luke C. Carey PhD
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  3. Nancy K. Valego PhD
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  4. Jingfang Liu PhD
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  5. James C. Rose PhD
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Corresponding author

Correspondence to James C. Rose PhD.

Additional information

Supported by National Insnitutes of Health Grant No. HD 17644

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Chen, K., Carey, L.C., Valego, N.K. et al. Combined Thyroidectomy and Renal Denervation Suppress Renin Expression and Secretion in Fetal Sheep. Reprod. Sci. 13, 604–609 (2006). https://doi.org/10.1016/j.jsgi.2006.07.006

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