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Nuchal Edema and Venous-Lymphatic Phenotype Disturbance in Human Fetuses and Mouse Embryos With Aneuploidy

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Abstract

Objective

Nuchal edema (NE) is a clinical indicator for aneuploidy, cardiovascular anomalies, and several genetic syndromes. Its etiology, however, is unknown. In the nuchal area, the endothelium of the jugular lymphatic sacs (JLS) develops by budding from the blood vascular endothelium of the cardinal veins. Abnormal distension of the jugular sacs is associated with NE. We hypothesize that a disturbed lymphatic endothelial differentiation and sac formation causes NE. We investigated endothelial differentiation of the jugular lymphatic system in human and mouse species with NE.

Methods

Aneuploid human fetuses (trisomy 21; trisomy 18) were compared with euploid controls (gestational age 12 to 18 weeks). Trisomy 16 mouse embryos were compared with wild type controls (embryonic day 10 to 18). Trisomy 16 mice are considered an animal model for human trisomy 21. Endothelial differentiation was investigated by immunohistochemistry using lymphatic markers (prox-1, podoplanin, lymphatic vessel endothelial hyaluronan receptor [LYVE]-1) and en blood vessel markers (neuropilin [NP]-1 and ligand vascular endothelial growth factor [VEGF]-A). Smooth muscle actin (SMA) was included as a smooth muscle cell marker.

Results

We report a disturbed venous-lymphatic phenotype in aneuploid human fetuses and mouse embryos with enlarged jugular sacs and NE. Our results show absent or diminished expression of the lymphatic markers Prox-1 and podoplanin in the enlarged jugular sac, while LYVE-1 expression was normal. Additionally, the enlarged fLS showed blood vessel characteristics, including increased NP-1 and VEGF-A expression. The lumen contained blood cells and smooth muscle cells lined the wall. CONCLUSION: A loss of lymphatic identity seems to be the underlying cause for clinical NE. Also, abnormal endothelial differentiation provides a link to the cardiovascular anomalies associated with NE.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, VU University Medical Center, Amsterdam, the Netherlands

    Mireille N. Bekker MD, Nynke M. S. van den Akker, Margot M. Bartelings MD, PhD, Jenny B. Arkesteijn, Sigrid G. L. Fischer, Japke A. E. Polman, Monique C. Haak MD, PhD, Sandra Webb PhD, Robert E. Poelmann PhD, John M. G. van Vugt MD, PhD & Adriana C. Gittenberger-de Groot PhD

  2. Department of Anatomy and Embryology, Leiden University Medical Center, P.O. Box 9602, 2300 RC, Leiden, the Netherlands

    Mireille N. Bekker MD, Nynke M. S. van den Akker, Margot M. Bartelings MD, PhD, Jenny B. Arkesteijn, Sigrid G. L. Fischer, Japke A. E. Polman, Monique C. Haak MD, PhD, Sandra Webb PhD, Robert E. Poelmann PhD, John M. G. van Vugt MD, PhD & Adriana C. Gittenberger-de Groot PhD

  3. Division of Basic Medical Sciences, Anatomy and Developmental Biology, St. George Hospital Medical School, London, UK

    Mireille N. Bekker MD, Nynke M. S. van den Akker, Margot M. Bartelings MD, PhD, Jenny B. Arkesteijn, Sigrid G. L. Fischer, Japke A. E. Polman, Monique C. Haak MD, PhD, Sandra Webb PhD, Robert E. Poelmann PhD, John M. G. van Vugt MD, PhD & Adriana C. Gittenberger-de Groot PhD

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  1. Mireille N. Bekker MD
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  2. Nynke M. S. van den Akker
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  3. Margot M. Bartelings MD, PhD
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  7. Monique C. Haak MD, PhD
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Corresponding author

Correspondence to Adriana C. Gittenberger-de Groot PhD.

Additional information

The authors thank J. Lens and S. Blankevoort for the graphics and layout and B. Wisse for technical assistance.

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Bekker, M.N., van den Akker, N.M.S., Bartelings, M.M. et al. Nuchal Edema and Venous-Lymphatic Phenotype Disturbance in Human Fetuses and Mouse Embryos With Aneuploidy. Reprod. Sci. 13, 209–216 (2006). https://doi.org/10.1016/j.jsgi.2006.02.003

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  • DOI: https://doi.org/10.1016/j.jsgi.2006.02.003

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