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Absence of In Vivo Generalized Pro-Inflammatory Endothelial Activation in Severe, Early-Onset Preeclampsia

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Abstract

Objective

At present it is unclear whether endothelial activation is systematically present in preeclampsia or restricted to specialized vascular beds. Therefore, this study aimed to investigate the presence of generalized proinflammatory endothelial activation in severe, early-onset preeclampsia in vivo.

Methods

During caesarean section, biopsies were obtained from abdominal subcutaneous fat, abdominal fascia, and myometrium from 11 severe, early-onset preeclamptic and 19 healthy pregnant women. Prior to caesarean, section plasma levels of von Willebrand Factor (vWF), sVCAM-1, and C-reactive protein (CRP) were measured by ELISA. Consecutive cryostat sections were stained immunohistochemically for CD31, E-selectin, VCAM-1, and ICAM-1. For subcutaneous fat tissue, endothelial gene expression levels of E-selectin, VCAM-1, ICAM-1, endothelin-1 (ET-1), and endothelial nitric oxide synthase (eNOS) were quantified by real-time RT-PCR, using normalization to the endothelium-specific housekeeping genes CD31 and VE-cadherin.

Results

Plasma levels of vWF, sVCAM-1, and CRP were elevated in the preeclampsia group compared to the control group, indicating enhanced endothelial activation and inflammatory response in the severely diseased preeclamptic women. By immunohistochemical analysis, no E-selectin and VCAM-1 expression could be detected in, and no differences in endothelial ICAM-1 staining could be observed between the preeclampsia and the control group for all tissues studied. Endothelial gene expression levels of E-selectin, VCAM-1, ICAM-1, ET-1, and eNOS were comparable between the preeclampsia and control group.

Conclusion

Protein and gene expression analysis of E-selectin, VCAM-1, ICAM-1, ET-1, and eNOS, key mediators involved in pro-inflammatory endothelial activation, could not identify endothelial activation in severe, early-onset preeclampsia in the tissues studied. However, elevated plasma levels of markers of endothelial activation and inflammation were observed. These results may suggest that in severe, early-onset preeclampsia pro-inflammatory endothelial cell activation is not a generalized phenomenon, but is likely restricted to (possibly organ-specific) specialized vascular beds.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Room Y4.240, University Medical Center Groningen, Hanzeplein 1, Postbox 30.001, 9700 RB, Groningen, The Netherlands

    Rogier B. Donker MSc, Grietje Molema PhD, Marijke M. Faas PhD, Cees G. M. Kallenberg MD, PhD, Maria G. van Pampus MD, PhD, Albertus Timmer MD, PhD & Jan G. Aarnoudse MD, PhD

Authors
  1. Rogier B. Donker MSc
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  2. Grietje Molema PhD
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  3. Marijke M. Faas PhD
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  4. Cees G. M. Kallenberg MD, PhD
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  5. Maria G. van Pampus MD, PhD
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  6. Albertus Timmer MD, PhD
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  7. Jan G. Aarnoudse MD, PhD
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Corresponding author

Correspondence to Rogier B. Donker MSc.

Additional information

Supported by the Groningen University Institute for Drug Exploration (GUIDE) and the Jan Kornelis de Cock Foundation, Project No. 04-06.

The authors thank Henk E. Moorlag, Department of Pathology and Laboratory Medicine, and Johan Bijzet, Department of Clinical Immunology, for expert technical assistance, and Sarah Buddeke and Patrick Vischjager for assistance in clinic and laboratory.

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Donker, R.B., Molema, G., Faas, M.M. et al. Absence of In Vivo Generalized Pro-Inflammatory Endothelial Activation in Severe, Early-Onset Preeclampsia. Reprod. Sci. 12, 518–528 (2005). https://doi.org/10.1016/j.jsgi.2005.06.007

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  • DOI: https://doi.org/10.1016/j.jsgi.2005.06.007

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