Abstract
Asparagine linked glycans (N-glycans) are important in biological processes. Yet, their structural complexity and lack of databases hinder progress in glycomics and glycobiology. We present a way for in silico generation of very large N-glycan structure databases and their use in high throughput composition and primary structure determination of N-glycans attached to peptides, based on CID (collision induced dissociation) MS/MS (tandem mass spectrometric data). The database and the integrated search engine is called Glyquest and is available to the glycomics community.
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Published online May 29, 2009
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Gao, H.Y. Generation of asparagine-linked glycan structure databases and their use. J Am Soc Mass Spectrom 20, 1739–1742 (2009). https://doi.org/10.1016/j.jasms.2009.05.012
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DOI: https://doi.org/10.1016/j.jasms.2009.05.012