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An ion trap-ion mobility-time of flight mass spectrometer with three ion sources for ion/ion reactions
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  • Published: 03 May 2009

An ion trap-ion mobility-time of flight mass spectrometer with three ion sources for ion/ion reactions

  • Qin Zhao1,
  • Matthew W. Soyk1,
  • Gregg M. Schieffer1,
  • Katrin Fuhrer3,
  • Marc M. Gonin3,
  • R. S. Houk2 &
  • …
  • Ethan R. Badman1 nAff4 

Journal of the American Society for Mass Spectrometry volume 20, pages 1549–1561 (2009)Cite this article

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Abstract

This instrument combines the capabilities of ion/ion reactions with ion mobility (IM) and time-of-flight (TOF) measurements for conformation studies and top-down analysis of large biomolecules. Ubiquitin ions from either of two electrospray ionization (ESI) sources are stored in a three dimensional (3D) ion trap (IT) and reacted with negative ions from atmospheric sampling glow discharge ionization (ASGDI). The proton transfer reaction products are then separated by IM and analyzed via a TOF mass analyzer. In this way, ubiquitin +7 ions are converted to lower charge states down to +1; the ions in lower charge states tend to be in compact conformations with cross sections down to ∼880 Å2. The duration and magnitude of the ion ejection pulse on the IT exit and the entrance voltage on the IM drift tube can affect the measured distribution of conformers for ubiquitin +7 and +6. Alternatively, protein ions are fragmented by collision-induced dissociation (CID) in the IT, followed by ion/ion reactions to reduce the charge states of the CID product ions, thus simplifying assignment of charge states and fragments using the mobility-resolved tandem mass spectrum. Instrument characteristics and the use of a new ion trap controller and software modifications to control the entire instrument are described.

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Author notes

  1. Ethan R. Badman

    Present address: Hoffmann-La Roche Inc., Nonclinical Safety, 340 Kingsland St., 07110, Nutley, NJ, USA

Authors and Affiliations

  1. Department of Chemistry, Iowa State University, Ames, Iowa, USA

    Qin Zhao, Matthew W. Soyk, Gregg M. Schieffer & Ethan R. Badman

  2. Ames Laboratory, U.S. Department of Energy, Iowa State University, Ames, Iowa, USA

    R. S. Houk

  3. Tofwerk AG, Thun, Switzerland

    Katrin Fuhrer & Marc M. Gonin

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  1. Qin Zhao
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These authors contributed equally to this work.

Published online May 3, 2009

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Zhao, Q., Soyk, M.W., Schieffer, G.M. et al. An ion trap-ion mobility-time of flight mass spectrometer with three ion sources for ion/ion reactions. J Am Soc Mass Spectrom 20, 1549–1561 (2009). https://doi.org/10.1016/j.jasms.2009.04.014

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  • Received: 07 January 2009

  • Revised: 17 April 2009

  • Accepted: 17 April 2009

  • Published: 03 May 2009

  • Issue Date: August 2009

  • DOI: https://doi.org/10.1016/j.jasms.2009.04.014

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Keywords

  • Charge State
  • Drift Tube
  • Lower Charge State
  • Injection Voltage
  • Trap Exit
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