Abstract
The design and implementation of a radio frequency acoustic desorption ionization (RADIO) source has been demonstrated for the analysis of multiply charged peptides and proteins. One µL aliquots of melittin, BNP-32, and ubiquitin (∼1 µg of analyte) were deposited onto a quartz crystal microbalance (QCM) electrode before radio frequency actuation for desorption. Continuous electrospray parallel to/above the sampling surface enabled the ionization of desorbed species. Detection by a hybrid linear ion trap Fourier transform ion cyclotron resonance mass spectrometer confirmed the intact and dissociated species observed during MS and MS/MS experiments, respectively.
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Published online December 7, 2008
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Dixon, R.B., Sampson, J.S. & Muddiman, D.C. Generation of multiply charged peptides and proteins by radio frequency acoustic desorption and ionization for mass spectrometric detection. J Am Soc Mass Spectrom 20, 597–600 (2009). https://doi.org/10.1016/j.jasms.2008.11.024
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DOI: https://doi.org/10.1016/j.jasms.2008.11.024