Abstract
Ion/ion charge inversion via multiple proton transfer reactions occurs via a long-lived intermediate. The intermediate can be observed if its lifetime is long relative to mechanisms for removal of excess energy (i.e., emission and collisional stabilization). The likelihood for formation of a stabilized intermediate is a function of characteristics of the reagent and analyte ions. This work is focused on the role acidic and basic sites of a deprotonated peptide play in the formation of a stabilized intermediate upon charge inversion with multiply protonated polypropyleniminediaminobutane dendrimers. A group of model peptides based on leucine enkephalin was used, which included YGGFL, YGGFLF, YGGFLK, YGGFLR and YGGFLH as well as methyl esterified and acetylated versions. Results showed that peptides containing basic amino acid residues charge inverted primarily by proton transfer from the DAB dendrimer to the peptide, whereas peptides without basic amino acids charge inverted primarily by complex formation with the DAB dendrimer. The modified versions of the peptides highlighted the importance of the presence of the C-terminus as well as the basicity of the peptide in the observation of a stabilized intermediate. These results provide new insights into the nature of the interactions that occur in the charge inversion of polypeptide anions via ion/ion reactions.
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Published online September 5, 2008
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Emory, J.F., McLuckey, S.A. The role of amino acid composition in the charge inversion of deprotonated peptides via gas-phase ion/ion reactions. J Am Soc Mass Spectrom 20, 180–187 (2009). https://doi.org/10.1016/j.jasms.2008.08.015
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DOI: https://doi.org/10.1016/j.jasms.2008.08.015