Abstract
Sulfopeptides can be misassigned as phosphopeptides because of the isobaric nature of the sulfo- and the phosphomoieties. Instruments having the ability to measure mass with high accuracy may be employed to distinguish these moieties based on their mass defect (the sulfo-group is 9 mmu lighter than the phosphomoiety). However, the assignment of the exact site(s) of post-translational modification is required to probe biological function. We have reported earlier that peptides with identical sequences containing either O-sulfo- or O-phospho-modifications display different fragmentation behavior (K. F. Medzihradszky et al., Mol. Cell. Proteom. 2004, 3, 429–440). We have also established that O-sulfo moieties are susceptible to side-chain fragmentation during collision-induced dissociation. Our present study provides evidence that neutral SO3 losses can also occur in electron capture dissociation and electron-transfer dissociation experiments. We also report that such neutral losses may be reduced by fragmenting peptide-alkali metal adducts, such as sodiated or potassiated peptides.
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Published online June 19, 2007
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Medzihradszky, K.F., Guan, S., Maltby, D.A. et al. Sulfopeptide fragmentation in electron-capture and electron-transfer dissociation. J Am Soc Mass Spectrom 18, 1617–1624 (2007). https://doi.org/10.1016/j.jasms.2007.06.002
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DOI: https://doi.org/10.1016/j.jasms.2007.06.002