Abstract
A new Fourier transform ion cyclotron resonance mass spectrometer based on a permanent magnet with an atmospheric pressure ionization source was designed and constructed. A mass resolving power (full-width-at-half-maximum) of up to 80,000 in the electron ionization mode and 25,000 in the electrospray mode was obtained. Also, a mass measurement accuracy at low-ppm level has been demonstrated for peptide mixtures in a mass range of up to 1200 m/z in the isotopically resolved mass spectra.
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Marshall, A. G.; Hendrickson, C. L.; Jackson, G. S. Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: A Primer. Mass Spectrom. Rev. 1998, 17(1), 1–35.
Bogdanov, B.; Smith, R. D. Proteomics by FTICR Mass Spectrometry: Top Down and Bottom Up. Mass Spectrom. Rev. 2005, 24(2), 168–200.
Zhang, L. K.; Rempel, D. L.; Pramanik, B. N.; Gross, M. L. Accurate Mass Measurements by Fourier Transform Mass Spectrometry. Mass Spectrom. Rev. 2005, 24, 286–309.
Gorshkov, M. V.; Udseth, H. R.; Anderson, G. A.; Smith, R. D. High Performance Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at Low Magnetic Field. Eur. J. Mass Spectrom. 2002, 8, 169–176.
Wu, Q.; Anderson, G. A.; Udseth, H. R.; Sherma, M. G.; Van Orden, S.; Chen, R.; Hofstadler, S. A.; Gorshkov, M. V.; Mitchell, D. W.; Rockwood, A. L.; Smith, R. D. A High Performance Low Magnetic Field Internal Electrospray Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometer. J. Am. Soc. Mass Spectrom. 1996, 7, 915–922.
Dietrich, D. D.; Keville, R. F. Electron Source for a Mini-Ion Trap Mass Spectrometer; U.S. Patent 5,477,046; 1995.
Zeller, L. C.; Kennady, J. M.; Kenttamaa, H.; Campana, J. E. Characterization of a Small FT-ICR Mass Spectrometer Based on a Permanent Magnet. Anal. Chem. 1993, 65, 2116–2118.
Jones, J. J.; Wilkins, C. L. Bacterial Pyrolysis Products Analyzed Using a Portable High Resolution 1 tesla Fourier Transform Mass Spectrometer. Proceedings of the 52nd ASMS Conference on Mass Spectrometry and Allied Topics; ashville, TN, 2004.
Rimkus, W. V.; Davis, D. V.; Gallaher, K. Learn FTMS Fundamentals and Quantitation on an Inexpensive Rugged Machine—Save Your High Powered FTMS for the Experienced User. Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics; San Antonio, TX, 2005.
Mauclaire, G.; Lemaire, J.; Boissel, P.; Bellec, G.; Heninger, M. MICRA: A Compact Permanent Magnet Fourier Transform Ion Cyclotron Resonance Mass Spectrometer. Eur. J. Mass Spectrom. 2004, 10(2), 155–162.
Gorshkov, M. V. Permanent Magnet Structure with Axial Access for Spectroscopy Applications; U.S. Patent Application 20,060,232,368; 2005.
Tarasova, I. A.; Tolmachev, D. A.; Vilkov, A. N.; Doroshenko, V. M.; Gorshkov, M. V. Permanent Magnet Assembly for Ion Cyclotron Resonance Mass Spectrometer with Atmospheric Pressure Ionization Sources. Mass Spectrometriya 2006, 3(2), 89–100.
Moskowitz, L. R. Permanent Magnet Design and Application Handbook; Krieger Publishing Co.: Malabar, FL, 1995, pp. 548–578.
Gamage, C. M.; Misharin, A. S.; Vilkov, A. N.; Gorshkov, M. V.; Doroshenko, V. M. ECD and CID of Peptide Ions in a Hybrid Permanent Magnet FT-ICR Mass Spectrometer. Proceedings of the 55th ASMS Conference on Mass Spectrometry and Allied Topics; Indianapolis, IN, 2007.
Dunbar, R. C.; Chen, J. H.; Hays, J. D. Magnetron Motion of Ions in the Cubical ICR Cell. Int. J. Mass Spectrom. Ion Processes 1984, 57, 39–56.
Beu, S. C.; Laude, D. A. Radial Ion Transport Due to Resistive-Wall Destabilization in Fourier Transform Mass Spectrometry. Int. J. Mass Spectrom. Ion Processes 1991, 108, 255–268.
White, R. L.; Ledford, E. B., Jr.; Ghaderi, S.; Wilkins, C. L.; Gross, M. L. Resolution and Signal-to-Noise in Fourier Transform Mass Spectrometry. Anal. Chem. 1980, 52(9), 1525–1527.
Guan, S.; Wahl, M. C.; Marshall, A. G. Elimination of Frequency Drift from Fourier Transform Ion Cyclotron Resonance Mass Spectra by Digital Quadrature Heterodyning: Ultrahigh Mass Resolving Power for Laser-Desorbed Molecules. Anal. Chem. 1993, 65(24), 3647–3653.
Bruce, J. E.; Anderson, G. A.; Hofstadler, S. A.; Winger, B. E.; Smith, R. D. Time-Base Modulation for the Correction of Cyclotron Frequency Shifts Observed in Long-Lived Transients from Fourier-transform Ion-Cyclotron-Resonance Mass Spectrometry of Electrosprayed Biopolymers. Rapid Commun. Mass Spectrom. 1993, 7, 700–703.
Guan, S.; Wahl, M. C.; Wood, T. D.; Marshall, A. G. Enhanced Mass Resolving Power, Sensitivity, and Selectivity in Laser Desorption Fourier Transform Ion Cyclotron Resonance Mass Spectrometry by Ion Axialization and Cooling. Anal. Chem. 1993, 65(13), 1753–1757.
Vilkov, A. N.; Gamage, C.; Oktem, B.; Shanbhag, S.; Doroshenko, V. M.; Tarasova, I. A.; Tolmachev, D. A.; Gorshkov, M. V. Atmospheric Pressure Ionization Permanent Magnet FTICR Mass Spectrometer: A Way to High Performance Mass Spectrometry in a Bench-Top Size. Proceedings of the 54th ASMS Conference on Mass Spectrometry and Allied Topics; Seattle, WA, 2006.
Ledford, E. B., Jr.; Rempel, D. L.; Gross, M. L. Space Charge Effects in Fourier Transform Mass Spectrometry: Mass calibration. Anal. Chem. 1984, 56(14), 2744–2748.
Gorshkov, M. V.; Zubarev, R. A. Linear Ion Trap Mass Spectrometer LTQ: Testing the Limits in Mass Accuracy and Resolution. Proceedings of the 53rd ASMS Conference on Mass Spectrometry and Allied Topics; San Antonio, TX, 2005.
Mitchell, D. W.; Smith, R. D. Cyclotron Motion of Two Coulombically Interacting Ion Clouds with Implications to Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry. Phys. Rev. E 1995, 52(4), 4366–4386.
Peurrung, A. J.; Kouzes, R. T. Long-Term Coherence of the Cyclotron Mode in a Trapped Ion Cloud. Phys. Rev. E 1994, 49(5), 4362–4368.
Belov, M. E.; Zhang, R.; Strittmatter, E. F.; Prior, D. C.; Tang, K.; Smith, R. D. Automated Gain Control and Internal Calibration with External Ion Accumulation Capillary Liquid Chromatography-Electrospray Ionization Fourier Transform Ion Cyclotron Resonance. Anal. Chem. 2003, 75(16), 4195–4205.
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Published online May 24, 2007
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Vilkov, A.N., Gamage, C.M., Misharin, A.S. et al. Atmospheric pressure ionization permanent magnet fourier transform ion cyclotron resonance mass spectrometry. J Am Soc Mass Spectrom 18, 1552–1558 (2007). https://doi.org/10.1016/j.jasms.2007.05.010
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DOI: https://doi.org/10.1016/j.jasms.2007.05.010