Abstract
We report the use of fluorescence spectroscopy to investigate the amount of material removed from a PTFE surface and detected during desorption electrospray ionization (DESI) mass spectrometry measurements. The fluorescence intensity before and after DESI analysis of rhodamine 6G is used to determine the amount of material removed from the surface per mass spectrum. Calculations indicate low attomole amounts are removed per linear ion trap mass spectrum.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M. Electrospray Ionization for Mass-Spectrometry of Large Biomolecules. Science 1989, 246, 64–71.
Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M. Electrospray Ionization—Principles and Practice. Mass Spectrom. Rev. 1990, 9, 37–70.
Karas, M.; Hillenkamp, F. Laser Desorption: Ionization of Proteins with Molecular Masses Exceeding 10,000 Daltons. Anal. Chem. 1988, 60, 2299–2301.
Tanaka, K.; Ido, Y.; Akita, S.; Yoshida, Y.; Yoshida, T. Protein and Polymer Analysis up to m/z 100,000 by Laser Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 1988, 2, 151–153.
McEwen, C. N.; McKay, R. G.; Larsen, B. S. Analysis of Solids, Liquids, and Biological Tissues Using Solids Probe Introduction at Atmospheric Pressure on Commercial LC/MS Instruments. Anal. Chem. 2005, 77, 7826–7831.
Cody, R. B.; Laramee, J. A.; Durst, H. D. Versatile New Ion Source for the Analysis of Materials in Open Air under Ambient Conditions. Anal. Chem. 2005, 77, 2297–2302.
Shiea, J.; Huang, M. Z.; Hsu, H. J.; Lee, C. Y.; Yuan, C. H.; Beech, I.; Sunner, J. Electrospray-assisted Laser Desorption/Ionization Mass Spectrometry for Direct Ambient Analysis of Solids. Rapid Commun. Mass Spectrom. 2005, 19, 3701–3704.
Huang, M. Z.; Hsu, H. J.; Lee, L. Y.; Jeng, J. Y.; Shiea, L. T. Direct Protein Detection from Biological Media through Electrospray-assisted Laser Desorption Ionization/Mass Spectrometry. J. Proteome Res. 2006, 5, 1107–1116.
Sampson, J. S.; Hawkridge, A. M.; Muddiman, D. C. Generation and Detection of Multiply-charged Peptides and Proteins by Matrix-assisted Laser Desorption Electrospray Ionization (MALDESI) Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2006, 17, 1712–1716.
Sampson, J. S.; Hawkridge, A. M.; Muddiman, D. C. Direct Characterization of Intact Polypeptides by Matrix Assisted Laser Desorption Electrospray Ionization (MALDESI) Quadrupole Fourier Transfrom Ion Cyclotron Resonance Mass Spectrometry. Rapid Commun. Mass Spectrom. 2007, 21, 1150–1154.
Takats, Z.; Wiseman, J. M.; Gologan, B.; Cooks, R. G. Mass Spectrometry Sampling under Ambient Conditions with Desorption Electrospray Ionization. Science. 2004, 306, 471–473.
Cooks, R. G.; Ouyang, Z.; Takats, Z.; Wiseman, J. M. Ambient Mass Spectrometry. Science. 2006, 311, 1566–1570.
Hu, Q. Z.; Talaty, N.; Noll, R. J.; Cooks, R. G. Desorption Electrospray Ionization Using an Orbitrap Mass Spectrometer: Exact Mass Measurements on Drugs and Peptides. Rapid Commun. Mass Spectrom. 2006, 20, 3403–3408.
Bereman, M. S.; Nyadong, L.; Fernandez, F. M.; Muddiman, D. C. Direct High-resolution Peptide and Protein Analysis by Desorption Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. Rapid Commun. Mass Spectrom. 2006, 20, 3409–3411.
Van Berkel, G. J.; Ford, M. J.; Deibel, M. A. Thin-layer Chromatography and Mass Spectrometry Coupled Using Desorption Electrospray Ionization. Anal. Chem. 2005, 77, 1207–1215.
Weston, D. J.; Bateman, R.; Wilson, I. D.; Wood, T. R.; Creaser, C. S. Direct Analysis of Pharmaceutical Drug Formulations Using Ion Mobility Spectrometry/Quadrupole-Time-of-Flight Mass Spectrometry Combined with Desorption Electrospray Ionization. Anal. Chem. 2005, 77, 7572–7580.
Williams, J. P.; Scrivens, J. H. Rapid Accurate Mass Desorption Electrospray Ionization Tandem Mass Spectrometry of Pharmaceutical Samples. Rapid Commun. Mass Spectrom. 2005, 19, 3643–3650.
Takats, Z.; Wiseman, J. M.; Cooks, R. G. Ambient Mass Spectrometry Using Desorption Electrospray Ionization (DESI): Instrumentation, Mechanisms and Applications in Forensics, Chemistry, and Biology. J. Mass Spectrom. 2005, 40, 1261–1275.
Van Berkel, G. J.; Kertesz, V. Automated Sampling and Imaging of Analytes Separated on Thin-layer Chromatography Plates Using Desorption Electrospray Ionization Mass Spectrometry. Anal. Chem. 2006, 78, 4938–4944.
Smith, R. D.; Loo, J. A.; Edmonds, C. G.; Barinaga, C. J.; Udseth, H. R. New Developments in Biochemical Mass-Spectrometry—Electrospray Ionization. Anal. Chem. 1990, 62, 882–899.
Zook, D. R.; Bruins, A. P. On Cluster Ions, Ion Transmission, and Linear Dynamic Range Limitations in Electrospray (Ionspray) Mass Spectrometry. Int. J. Mass Spectrom. Ion Processes. 1997, 162, 129–147.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published online April 19, 2007
Rights and permissions
About this article
Cite this article
Bereman, M.S., Muddiman, D.C. Detection of Attomole Amounts of Analyte by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) Determined Using Fluorescence Spectroscopy. J Am Soc Mass Spectrom 18, 1093–1096 (2007). https://doi.org/10.1016/j.jasms.2007.03.006
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1016/j.jasms.2007.03.006