Abstract
A pulsed triple ionization source, using a common atmosphere/vacuum interface and ion path, has been developed to generate different types of ions for sequential ion/ion reaction experiments in a linear ion trap-based tandem mass spectrometer. The triple ionization source typically consists of a nano-electrospray emitter for analyte formation and two other emitters, an electrospray emitter and an atmospheric pressure chemical ionization emitter or a second nano-electrospray emitter for formation of the two different reagent ions. The three emitters are positioned in a parallel fashion close to the sampling orifice of the tandem mass spectrometer. The potentials applied to each emitter are sequentially pulsed so that desired ions are generated separately in time and space. Sequential ion/ion reactions take place after analyte ions of interest and different set of reagent ions are sequentially injected into a linear ion trap, where axial trapping is effected by applying an auxiliary radio frequency voltage to the end lenses. The pulsed triple ionization source allows independent optimization of each emitter and can be readily coupled to any atmospheric pressure ionization interface with no need for instrument modifications, provided the potentials required to transmit the ion polarity of interest can be synchronized with the emitter potentials. Several sequential ion/ion reactions examples are demonstrated to illustrate the analytical usefulness of the triple ionization source in the study of gas-phase ion/ion chemistry.
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McLuckey, S. A.; Stephenson, J. L. Jr. Ion/ion chemistry of high-mass multiply charged ions. Mass Spectrom. Rev. 1998, 17, 369–407.
Pitteri, S. J.; McLuckey, S. A. Recent developments in the ion/ion chemistry of high-mass multiply charged ions. Mass Spectrom. Rev. 2005, 24, 931–958.
Stephenson, J. L. Jr.; McLuckey, S. A. Ion/ion proton transfer reactions for protein mixture analysis. Anal. Chem. 1996, 68, 4026–4032.
Stephenson, J. L. Jr.; McLuckey, S. A. Simplification of product ion spectra derived from multiply charged parent ions via ion/ion chemistry. Anal. Chem. 1998, 70, 3533–3544.
McLuckey, S. A.; Reid, G. E.; Wells, J. M. Ion parking during ion/ion reactions in electrodynamic ion traps. Anal. Chem. 2002, 74, 336–346.
Chrisman, P. A.; Pitteri, S. J.; McLuckey, S. A. Parallel ion parking of protein mixtures. Anal. Chem. 2006, 78, 310–316.
Coon, J. J.; Syka, J. E. P.; Schwartz, J. C.; Shabanowitz, J.; Hunt, D. F. Anion dependence in the partitioning between proton and electron transfer in ion/ion reactions. Int. J. Mass Spectrom. 2004, 236, 33–42.
Syka, J. E.; Coon, J. J.; Schroeder, M. J.; Shabanowitz, J.; Hunt, D. F. Peptide and protein sequence analysis by electron transfer dissociation mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 9528–9533.
Chrisman, P. A.; Pitteri, S. J.; Hogan, J. M.; McLuckey, S. A. SO −·2 electron transfer ion/ion reactions with disulfide linked polypeptide ions. J. Am. Soc. Mass. Spectrom. 2005, 16, 1020–1030.
Hogan, J. M.; Pitteri, S. J.; Chrisman, P. A.; McLuckey, S. A. Complementary structural information from a tryptic N-linked glycopeptide via electron transfer ion/ion reactions and collision—induced dissociation. J. Proteome Res. 2005, 4, 628–632.
Pitteri, S. J.; Chrisman, P. A.; Hogan, J. M.; McLuckey, S. A. Electron transfer ion/ion reactions in a three-dimensional quadrupole ion trap: Reactions of doubly and triply protonated peptides with SO −·2 . Anal. Chem. 2005, 77, 1831–1839.
Ogorzalek-Loo, R. R.; Udseth, H. R.; Smith, R. D. A new approach for the study of gas-phase ion-ion reactions using electrospray ionization. J. Am. Soc. Mass Spectrom. 1992, 3, 695–705.
Ebeling, D. D.; Westphall, M. S.; Scalf, M.; Smith, L. M. Corona discharge in charge reduction electrospray mass spectrometry. Anal. Chem. 2000, 72, 5158–5161.
Frey, B. L.; Lin, Y.; Westphall, M. S.; Smith, L. M. Controlling gas-phase reactions for efficient charge reduction electrospray mass spectrometry of intact proteins. J. Am. Soc. Mass Spectrom. 2005, 16, 1876–1887.
Scalf, M.; Westphall, M. S.; Smith, L. M. Charge reduction electrospray mass spectrometry. Anal. Chem. 2000, 72, 52–60.
Scalf, M.; Westphall, M. S.; Krause, J.; Kaufman, S. L.; Smith, L. M. Controlling charge states of large ions. Science. 1999, 283, 194–197.
Ogorzalek-Loo, R. R.; Udseth, H. R.; Smith, R. D. Evidence of charge inversion in the reaction of singly charged anions with multiply charged macro-ions. J. Phys. Chem. 1991, 95, 6412–6415.
Mather, R. E.; Todd, J. F. J. The Quadrupole Ion Store (Quistor): VII. Simultaneous positive-negative ion mass-spectrometry. Int. J. Mass Spectrom. Ion Phys. 1980, 33, 159–165.
McLuckey, S. A.; Glish, G. L.; Van Berkel, G. J. Multiple stages of mass-spectrometry in a quadrupole ion trap mass-spectrometer—prerequisites. Int. J. Mass Spectrom. Ion Processes. 1991, 106, 213–235.
He, M.; McLuckey, S. A. Two ion/ion charge inversion steps to form a doubly protonated peptide from a singly protonated peptide in the gas phase. J. Am. Chem. Soc. 2003, 125, 7756–7757.
He, M.; McLuckey, S. A. Increasing the negative charge of a macro-anion in the gas phase via sequential charge inversion reactions. Anal. Chem. 2004, 76, 4189–4192.
Gunawardena, H. P.; Emory, J. F.; McLuckey, S. A. Phosphopeptide anion characterization via sequential charge inversion and electron-transfer dissociation. Anal. Chem. 2006, 78, 3788–3793.
Coon, J. J.; Ueberheide, B.; Syka, J. E.; Dryhurst, D. D.; Ausio, J.; Shabanowitz, J.; Hunt, D. F. Protein identification using sequential ion/ion reactions and tandem mass spectrometry. Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 9463–9468.
Herron, W. J.; Goeringer, D. E.; McLuckey, S. A. Gas-Phase Electron Transfer Reactions from Multiply-Charged Anions to Rare Gas Cations. J. Am. Chem. Soc. 1995, 117, 11555–11562.
Herron, W. J.; Goeringer, D. E.; McLuckey, S. A. Ion-ion reactions in the gas phase: Proton transfer reactions of protonated pyridine with multiply charged oligonucleotide anions. J. Am. Soc. Mass Spectrom. 1995, 6, 529–532.
Payne, A. H.; Glish, G. L. Gas-phase ion/ion interactions between peptides or proteins and iron ions in a quadrupole ion trap. Int. J. Mass Spectrom. 2001, 204.
Stephenson, J. L. Jr.; McLuckey, S. A. Adaptation of the Paul Trap for study of the reaction of multiply charged cations with singly charged anions. Int. J. Mass Spectrom. Ion Processes. 1997, 162, 89–106.
Reid, G. E.; Wells, J. M.; Badman, E. R.; McLuckey, S. A. Performance of a quadrupole ion trap mass spectrometer adapted for ion/ion reaction studies. Int. J. Mass Spectrom. 2003, 222, 243–258.
Wu, J.; Hager, J. W.; Xia, Y.; Londry, F. A.; McLuckey, S. A. Positive ion transmission mode ion/ion reactions in a hybrid linear ion trap. Anal. Chem. 2004, 76, 5006–5015.
Wells, J. M.; Chrisman, P. A.; McLuckey, S. A. “Dueling” ESI: Instrumentation to study ion/ion reactions of electrospray-generated cations and anions. J. Am. Soc. Mass Spectrom. 2002, 13, 614–622.
Badman, E. R.; Chrisman, P. A.; McLuckey, S. A. A quadrupole ion trap mass spectrometer with three independent ion sources for the study of gas-phase ion/ion reactions. Anal. Chem. 2002, 74, 6237–6243.
Xia, Y.; Liang, X.; McLuckey, S. A. Sonic spray as a dual polarity ion source for ion/ion reactions. Anal. Chem. 2005, 77, 3683–3689.
Xia, Y.; Liang, X.; McLuckey, S. A. Pulsed dual electrospray ionization for ion/ion reactions. J. Am. Soc. Mass Spectrom. 2005, 16, 1750–1756.
Liang, X.; Xia, Y.; McLuckey, S. A. Alternately pulsed nanoelectrospray ionization/atmospheric pressure chemical ionization for ion/ion reactions in an electrodynamic ion trap. Anal. Chem. 2006, 78, 3208–3212.
Hager, J. W. A new linear ion trap mass spectrometer. Rapid Commun. Mass Spectrom. 2002, 16, 512–526.
Xia, Y.; Wu, J.; McLuckey, S. A.; Londry, F. A.; Hager, J. W. Mutual storage mode ion/ion reactions in a hybrid linear ion trap. J. Am. Soc. Mass Spectrom. 2005, 16, 71–81.
Londry, F. A.; Hager, J. W. Mass selective axial ion ejection from a linear quadrupole ion trap. J. Am. Soc. Mass Spectrom. 2003, 14, 1130–1147.
Karas, M.; Hillenkamp, F. Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal. Chem. 1988, 60, 2299–2301.
Fenn, J. B.; Mann, M.; Meng, C. K.; Wong, S. F.; Whitehouse, C. M. Electrospray ionization for mass spectrometry of large biomolecules. Science. 1989, 246, 64–71.
Dongré, A. R.; Jones, J. L.; Somogyi, Á.; Wysocki, V. H. Influence of peptide composition, gas-phase basicity, and chemical modification on fragmentation efficiency: Evidence for the mobile proton model. J. Am. Chem. Soc. 1996, 118, 8365–8374.
Reid, G. E.; Wu, J.; Chrisman, P. A.; Wells, J. M.; McLuckey, S. A. Charge-state-dependent sequence analysis of protonated ubiquitin ions via ion trap tandem mass spectrometry. Anal. Chem. 2001, 73, 3274–3281.
Flora, J. W.; Muddiman, D. C. Selective, sensitive, and rapid phosphopeptide identification in enzymatic digests using ESI-FTICR-MS with infrared multiphoton dissociation. Anal. Chem. 2001, 73, 3305–3311.
Annan, R. S.; Carr, S. A. Phosphopeptide analysis by matrix-assisted laser desorption time-of-flight mass spectrometry. Anal. Chem. 1996, 68, 3413–3421.
Stone, D. H.; Hemling, M. E.; Carr, S. A.; Horn, D. M.; Lindh, I.; McLafferty, F. W. Phosphopeptide/phosphoprotein mapping by electron capture dissociation mass spectrometry. Anal. Chem. 2001, 73, 19–22.
Gunawardena, H. P.; He, M.; Chrisman, P. A.; Pitteri, S. J.; Hogan, J. M.; Hodges, B. D.; McLuckey, S. A. Electron transfer versus proton transfer in gas-phase ion/ion reactions of polyprotonated peptides. J. Am. Chem. Soc. 2005, 127, 12627–12639.
Ge, Y.; El-Naggar, M.; Sze, S. K.; Oh, H. B.; Begley, T. P.; McLafferty, F. W.; Boshoff, H.; Barry, C. E. III Top down characterization of secreted proteins from Mycobacterium tuberculosis by electron capture dissociation mass spectrometry. J. Am. Soc. Mass Spectrom. 2003, 14, 253–261.
Ge, Y.; Lawhorn, B. G.; ElNaggar, M.; Strauss, E.; Park, J. H.; Begley, T. P.; McLafferty, F. W. Top down characterization of larger proteins (45 kDa) by electron capture dissociation mass spectrometry. J. Am. Chem. Soc. 2002, 124, 672–678.
Sze, S. K.; Ge, Y.; Oh, H.; McLafferty, F. W. Top-down mass spectrometry of a 29-kDa protein for characterization of any posttranslational modification to within one residue. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 1774–1779.
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Published online November 13, 2006
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Liang, X., Han, H., Xia, Y. et al. A pulsed triple ionization source for sequential ion/ion reactions in an electrodynamic ion trap. J Am Soc Mass Spectrom 18, 369–376 (2007). https://doi.org/10.1016/j.jasms.2006.10.004
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DOI: https://doi.org/10.1016/j.jasms.2006.10.004