Abstract
A Microsoft Excel utility, HX-Express, that significantly accelerates the analysis of hydrogen exchange mass spectrometry data is described. HX-Express generates deuterium uptake and peak width plots from peaks in mass spectral data. Data analysis is intentionally semi-automated, requiring that the user find the peaks to be analyzed. The peaks are entered in the form of x, y lists of m/z versus intensity or can be directly imported from Waters MassLynx software. Analysis of data with HX-Express provides the same results as manual data processing but is substantially faster. In addition to speed, HX-Express provides and preserves visual and numeric displays of the analysis process for quality control.
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Published online August 22, 2006
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Weis, D.D., Engen, J.R. & Kass, I.J. Semi-automated data processing of hydrogen exchange mass spectra using HX-Express . The official journal of The American Society for Mass Spectrometry 17, 1700–1703 (2006). https://doi.org/10.1016/j.jasms.2006.07.025
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DOI: https://doi.org/10.1016/j.jasms.2006.07.025