Abstract
We have studied the mechanism of formation CN− secondary ions under Cs+ primary ion bombardment. We have synthesized 13C and 15N labeled polyglycine samples with the distance between the two labels and the local atomic environment of the 13C label systematically varied. We have measured four masses in parallel: 12C, 13C, and two of 12C14N, 13C14N, 12C15N, and 13C15N. We have calculated the 13C/12C isotope ratio, and the different combinations of the CN isotope ratios (27CN/26CN, 28CN/27CN, and 28CN/26CN). We have measured a high 13C15N − secondary ion current from the 13C and 15N labeled polyglycines, even when the 13C and 15N labels are separated. By comparing the magnitude of the varied combinations of isotope ratios among the samples with different labeling positions, we conclude the following: CN− formation is in large fraction due to recombination of C and N; the C=O double bond decreases the extent of CN− formation compared to the case where carbon is singly bonded to two hydrogen atoms; and double-labeling with 13C and 15N allows us to detect with high sensitivity the molecular ion 13C15N−.
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Lechene, C.; Hillion, F.; Cotanche, D. A.; McMahon, G.; Benson, D.; Kleinfeld, A. M.; Kampf, J. P.; Distel, D.; Luyten, Y.; Bonventre, J.; Hentschel, D.; Park, K. M.; Ito, S.; Schwartz, M.; Benichou, G.; Slodzian, G.; High resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry. J. Biol. 2006, in press.
McMahon, G.; Glassner, B. J.; Lechene, C. P.; Quantitative imaging of cells with multi-isotope imaging mass spectrometry (MIMS)—Nanoautography with stable isotope tracers. Proceedings of the 15th International Conference on Secondary Ion Mass Spectrometry—SIMS XV, Appl. Surf. Sci. 2005, in press.
Kleinfeld, A. M.; Kampf, J. P.; Lechene, C. Transport of 13C-oleate in adipocytes measured using multi-isotope imaging mass spectrometry. J. Am. Soc. Mass Spectrom. 2004, 15, 1572–1580.
Hallegot, P.; Peteranderl, R.; Lechene, C. In-situ imaging mass spectrometry analysis of melanin granules in the human hair shaft. J. Invest. Dermatol. 2004, 122, 381–386.
Hindie, E.; Coulomb, B.; Beaupain, R.; Galle, P. Mapping the cellular distribution of labeled molecules by SIMS microscopy. Biol. Cell. 1992, 74, 81–88.
Hindie, H.; Blaise, G.; Galle, P.; Origin of the CN− secondary ions emitted from biological tissue under 10 keV Cs+ bombardment. In Proceedings of the Secondary Ion Mass Spectrometry SIMS XII International Conference; Wiley: Chichester, UK, 1990; p 335.
Roberts, J. L.; Poulter, C. D. 2′3′5′-Tri-O-benzoyl [4- 13C] uridine: An efficient, regiospecific synthesis of the pyrimidine ring. J. Org. Chem. 1978, 43, 1547–1550.
Ott, D. G.; Synthesis with stable isotopes of carbonnitrogen and oxygen. John Wiley and Sons: New York, 1981; p 33–41.
Paquet, A. Introduction of 9-fluorenylmethyloxycarbonyl, trichloroethoxycarbonyl, and benzyloxycarbonyl amine protecting groups into O-unprotected hydroxyamino acids using succinimidyl carbonates. Can. J. Chem. 1982, 60, 976–980.
Peteranderl, R.; Lechene, C. Measurement of carbon and nitrogen stable isotope ratios in cultured cells. J. Am. Soc. Mass Spectrom. 2004, 15, 478–485.
Pauling, L. College Chemistry, 3rd ed.; W. H. Freeman and Company: San Francisco and London, 1989; 316.
Schoenheimer, R. The dynamic state of body constituents, The Edward K. Dunham lectures for the promotion of the medical sciences 1941; Harvard University Press: Cambridge, 1942; p 1.
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Published online June 5, 2006
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McMahon, G., Saint-Cyr, H.F., Lechene, C. et al. CN− secondary ions form by recombination as demonstrated using multi-isotope mass spectrometry of 13C- and 15N-labeled polyglycine. The official journal of The American Society for Mass Spectrometry 17, 1181–1187 (2006). https://doi.org/10.1016/j.jasms.2006.04.031
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DOI: https://doi.org/10.1016/j.jasms.2006.04.031