Abstract
A systematic study of the dissociation patterns of crosslinked peptides analyzed by tandem mass spectrometry is reported. A series of 11-mer peptides was designed around either a polyalanine or polyglycine scaffold with arginine at the C terminus. One or two lysine residues were included at various locations within the peptides to effect inter- or intra-molecular crosslinking, respectively. Crosslinked species were generated with four commonly used amine-specific chemical crosslinking reagents: disuccinimidyl suberate (DSS), disuccinimidyl tartarate (DST), dithiobis(succinimidylpropionate) (DSP), and disuccinimidyl glutarate (DSG). The influence of precursor charge state, location of crosslink, and specific crosslinking reagent on the MS/MS dissociation pattern was examined. Observed trends in the dissociation patterns obtained for these species will allow for improvements to software used in the automated interpretation of crosslinked peptide MS/MS data.
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Published online January 27, 2006
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Gaucher, S.P., Hadi, M.Z. & Young, M.M. Influence of crosslinker identity and position on gas-phase dissociation of lys-lys crosslinked peptides. The official journal of The American Society for Mass Spectrometry 17, 395–405 (2006). https://doi.org/10.1016/j.jasms.2005.11.023
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DOI: https://doi.org/10.1016/j.jasms.2005.11.023