Abstract
We report on the use of a jet disrupter electrode in an electrodynamic ion funnel as an electronic valve to regulate the intensity of the ion beam transmitted through the interface of a mass spectrometer in order to perform automatic gain control (AGC). The ion flux is determined by either directly detecting the ion current on the conductance limiting orifice of the ion funnel or using a short mass spectrometry acquisition. Based upon the ion flux intensity, the voltage of the jet disrupter is adjusted to alter the transmission efficiency of the ion funnel to provide a desired ion population to the mass analyzer. Ion beam regulation by an ion funnel is shown to provide control to within a few percent of a targeted ion intensity or abundance. The utility of ion funnel AGC was evaluated using a protein tryptic digest analyzed with liquid chromatography Fourier transform ion cyclotron resonance (LC-FTICR) mass spectrometry. The ion population in the ICR cell was accurately controlled to selected levels, which improved data quality and provided better mass measurement accuracy.
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Patel, P. H.; Suzuki, M.; Adman, E.; Shinkai, A.; Loeb, L. A. Prokaryotic DNA Polymerase I: Evolution, Structure, and “Base Flipping” Mechanism for Nucleotide Selection. J. Mol. Biol. 2001, 308, 823–837.
Pieper, R.; Gatlin, C. L.; Makusky, A. J.; Russo, P. S.; Schatz, C. R.; Miller, S. S.; Su, Q.; McGrath, A. M.; Estock, M. A.; Parmar, P. P.; Zhao, M.; Huang, S. T.; Zhou, J.; Wang, F.; Esquer-Blasco, R.; Anderson, N. L.; Taylor, J.; Steiner, S. Identification and Quantification of N-linked Glycoproteins using Hydrazide Chemistry, Stable-Isotope Labeling, and Mass Spectrometry. Proteomics 2003, 3, 422–432.
Gygi, S. P.; Rist, B.; Gerber, S. A.; Turecek, F.; Gelb, M. H.; Aebersold, R. Quantitative Analysis of Complex Protein Mixture using Isotope-Coded Affinity Tags. Nat. Biotechnol. 1999, 17, 994–999.
Pieper, R.; Su, Q.; Gatlin, C. L.; Huang, S.-T.; Anderson, N. L.; Steiner, S. Multi-Component Immunoaffinity Subtraction Chromatography: An Innovative Step Towards a Comprehensive Survey of the Human Plasma Proteome. Proteomics 2003, 3, 422–432.
Link, A. J. Multidimensional Peptide Separations in Proteomics. Trends Biotechnol. 2002, 20, S8-S13.
Wu, S.-L.; Amato, H.; Biringer, R.; Choudhary, G.; Shieh, P.; Hancock, W. S. Targeted Proteomics of Low-Level Proteins in Human Plasma by LC/MSn: Using Human Growth Hormone as a Model System. J. Proteome Res. 2002, 1, 459–465.
Harkewicz, R.; Belov, M. E.; Anderson, G. A.; Pasa-Tolic, L.; Masselon, C. D.; Prior, D. C.; Udseth, H. R.; Smith, R. D. ESI-FTICR Mass Spectrometry Employing Data-Dependant External Ion Selection and Accumulation. J. Am. Soc. Mass Spectrom. 2002, 13, 144–154.
Belov, M. E.; Zhang, R.; Strittmatter, E. F.; Prior, D. C.; Tang, K.; Smith, R. D. Automated Gain Control and Internal Calibration with External Ion Accumulation Capillary Liquid Chromatography-Electrospray Ionization-Fourier Transform Ion Cyclotron Resonance. Anal. Chem. 2003, 75, 4195–4205.
Schwartz, J. C.; Zhou, X.-G.; Bier, M. E. Method and apparatus of increasing dynamic range and sensitivity of a mass spectrometer. US Patent 5,572,022; 1996.
Belov, M. E.; Rakov, V. S.; Nikolaev, E. N.; Goshe, M. B.; Anderson, G. A.; Smith, R. D. Initial Implementation of External Accumulation Liquid Chromatography/Electrospray Ionization Fourier Transform Ion Cyclotron Resonance with Automated Gain Control. Rapid Commun. Mass Spectrom. 2003, 17, 627–636.
Senko, M.; Zabrouskov, V.; Lange, O.; Wieghaus, A.; Horning, S. LC/MS with External Calibration Mass Accuracies Approaching 100 ppb. Proceedings of the 52nd ASMS Conference on Mass Spectrometry; Nashville, TN, May 2004.
Taylor, P. K.; Amster, I. J. Space Charge Effects on Mass Accuracy for Multiply Charged Ions in ESI-FTICR. Int. J. Mass Spectrom. 2003, 222, 351–361.
Masselon, C.; Tolmachev, A. V.; Anderson, G. A.; Harkewicz, R.; Smith, R. D. Mass Measurement Errors Caused by “Local” Frequency Perturbations in FTICR Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2002, 13, 99–106.
Easterling, M. L.; Mize, T. H.; Amster, I. J. Routine Parts-per-Million Mass Accuracy for High-Mass Ions: Space-Charge Effects in MALDI FT-ICR. Anal. Chem. 1999, 71, 624–632.
Vedel, F.; Andre, J. Influence of Space Charge on the Computed Statistical Properties of Stored Ions Cooled by a Buffer Gas in a Quadrupole RF Trap. Phys. Rev. A 1984, 29, 2098–2101.
Cox, K. A.; Cleven, C. D.; Cooks, R. G. Mass Shifts and Local Space Charge Effects Observed in the Quadrupole Ion Trap at Higher Resolution. Int. J. Mass Spectrom. Ion Processes 1995, 144, 47–65.
Mathurin, J. C.; Gregoire, S.; Brunot, A.; Tabet, J. C.; March, R. E.; Catinella, S.; Traldi, P. Investigation of Space Charge Interactions which Arise During Simultaneous Confinement of Positive and Negative Ions in an Ion Trap Mass Spectrometer. J. Mass Spectrom. 1997, 32, 829–837.
Alheit, R.; Kleineidam, S.; Vedel, F.; Vedel, M.; Werth, G. Higher Order Non-Linear Resonances in a Paul Trap. Int. J. Mass Spectrom. Ion Processes 1996, 154, 155–169.
Cleven, C. D.; Cox, K. A.; Cooks, R. G.; Bier, M. E. Mass Shifts Due to Ion-Ion Interactions in a Quadrupole Ion-Trap Mass-Spectrometer. Rapid Commun. Mass Spectrom. 1994, 8, 451–454.
Takach, E. J.; Hines, W. M.; Patterson, D. H.; Juhasz, P.; Falick, A. M.; Vestal, M. L.; Martin, S. A. Accurate Mass Measurements using MALDI-TOF with Delayed Extraction. J. Protein Chem. 1997, 16, 363–369.
Dehmelt, H. G. Radiofrequency Spectroscopy of Stored Ions. I: Storage. Adv. Atom. Mol. Phys. 1967, 3, 53–72.
Belov, M. E.; Nikolaev, E. N.; Harkewicz, R.; Masselon, C. D.; Alving, K.; Smith, R. D. Ion Discrimination During Ion Accumulation in a Quadrupole Interface External to a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer. Int. J. Mass. Spectrom. 2001, 208, 205–225.
Sannes-Lowery, K.; Griffey, R. H.; Kruppa, G. H.; Speir, J. P.; Hofstadler, S. A. Multipole Storage Assisted Dissociation, a Novel in-Source Dissociation Technique for Electrospray Ionization Generated Ions. Rapid Commun. Mass Spectrom. 1998, 12, 1957–1961.
Hakansson, K.; Axelsson, J.; Palmblad, M.; Hakansson, P. Mechanistic Studies of Multipole Storage Assisted Dissociation. J. Am. Soc. Mass Spectrom. 2000, 11, 210–215.
Sannes-Lowery, K. A.; Hofstadler, S. A. Characterization of Multipole Storage Assisted Dissociation: Implications for Electrospray Ionization Mass Spectrometry Characterization of Biomolecules. J. Am. Soc. Mass Spectrom. 2000, 11, 1–7.
Tang, K.; Tolmachev, A. V.; Nikolaev, E.; Zhang, R.; Belov, M. E.; Udseth, H. R.; Smith, R. D. Independent Control of Ion Transmission in a Jet Disrupter Dual-Channel Ion Funnel Electrospray Ionization MS Interface. Anal. Chem. 2002, 74, 5431–5437.
Taeman, K.; Tolmachev, A. V.; Harkewicz, R.; Prior, D. C.; Anderson, G.; Udseth, H. R.; Smith, R. D.; Bailey, T. H.; Rakov, S.; Futrell, J. H. Design and Implementation of a New Electrodynamic Ion Funnel. Anal. Chem. 2000, 72, 2247–2255.
Shaffer, S. A.; Tang, K.; Anderson, G. A.; Prior, D. C.; Udseth, H. R.; Smith, R. D. A Novel Ion Funnel for Focusing Ions at Elevated Pressure Using Electrospray Ionization Mass Spectrometry. Rapid Commun. Mass Spectrom. 1997, 11, 1813–1817.
Shaffer, S. A.; Prior, D. C.; Anderson, G. A.; Udseth, H. R.; Smith, R. D. An Ion Funnel Interface for Improved Ion Focusing and Sensitivity Using Electrospray Ionization Mass Spectrometry. Anal. Chem. 1998, 70, 4111–4119.
Shaffer, S. A.; Tolmachev, A.; Prior, D. C.; Anderson, G. A.; Udseth, H. R.; Smith, R. D. Characterization of an Improved Electrodynamic Ion Funnel Interface for Electrospray Ionization Mass Spectrometry. Anal. Chem. 1999, 71, 2957–2964.
Kim, T.; Tang, K.; Udseth, H. R.; Smith, R. D. A Multicapillary Inlet Jet Disruption Electrodynamic Ion Funnel Interface for Improved Sensitivity Using Atmospheric Pressure Ion Sources. Anal. Chem. 2001, 73, 4162–4170.
Winger, B.; Light-Wahl, K.; Ogorzalek-Loo, R.; Udseth, H. R.; Smith, R. D. Observation and Implications of High Mass-to-Charge Ratio Ions from Electrospray Ionization Mass Spectrometry. J. Am. Soc. Mass Spectrom. 1993, 4, 536–545.
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Published online December 22, 2004
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Page, J.S., Bogdanov, B., Vilkov, A.N. et al. Automatic gain control in mass spectrometry using a jet disrupter electrode in an electrodynamic ion funnel. J Am Soc Mass Spectrom 16, 244–253 (2005). https://doi.org/10.1016/j.jasms.2004.11.003
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DOI: https://doi.org/10.1016/j.jasms.2004.11.003