Abstract
MALDI-TOF MS is utilized to perform quantitative analysis on synthetic polymers. Despite the inherent limitations of MALDI, good quantitative results have been obtained in the three sets of experiments described here. An internal standard with similar molecular properties as the analytes is introduced. Plots of relative integrated intensity ratios as a function of theoretical ratios of stoichiometry are drawn based on the results. The satisfactory slopes and correlation coefficients illustrated the practicality of quantitative measurement by MALDI-TOF MS.
Article PDF
Similar content being viewed by others
References
Tanaka, K.; Waki, H.; Yoshida, Y.; Yoshida, T. Protein and Polymer Analyses up to m/z 100,000 by Laser Ionization Time-of flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 1988, 2, 151–153.
Karas, M.; Hillenkamp, F. Laser Desorption Ionization of Proteins with Molecular Masses Exceeding 10,000 Daltons. Anal. Chem. 1988, 60, 2299–2301.
Zaluzec, E. J.; Gage, D. A.; Watson, J. T. Matrix-Assisted Laser Desorption Ionization Mass Spectrometry: Applications in Peptide and Protein Characterization. Protein Exp. Purif. 1995, 6, 109–123.
Roepstorff, P. MALDI-TOF Mass Spectrometry in Protein. Chem. Express 2000, 88, 81–97.
Nguyen, D. N.; Becker, G. W.; Riggin, R. M. Protein Mass Spectrometry: Applications to Analytical Biotechnology. J. Chromatogr. A. 1995, 705, 21–45.
Marie, A.; Fournier, F.; Tabet, J. C. Characterization of Synthetic Polymers by MALDI-TOF/MS: Investigation into New Methods of Sample Target Preparation and Consequence on Mass Spectrum Finger Print. Anal. Chem. 2000, 72, 5106–5114.
Wu, K. J.; Odom, R. W. Characterizing Synthetic Polymers by MALDI MS. Anal. Chem. 1998, 70, 456A-461A.
Busch, K. L. Suggested Journal Readings on Desorption Ionization Techniques. J. Mass Spectrom. 1995, 30, 233–237.
Schriemer, D. C.; Li, L. Detection of High Molecular Weight Polystyrene by MALDI Mass Spectrometry. Anal. Chem. 1996, 68, 2721–2725.
Jackson, A. T.; Yates, H. T.; Lindsay, C. I.; Didier, Y.; Segal, J. A.; Scrivens, J. H.; Critchley, G.; Brown, J. Utilizing Time-Lag Focusing Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry for the End Group Analysis of Synthetic Polymers. Rapid Commun. Mass Spectrom. 1997, 11, 520–526.
Hensley, P. Defining the Structure and Stability of Macromolecular Assemblies in Solution: The Re-emergence of Analytical Ultracentrifugation in Solution. Structure 1996, 4, 367.
McLafferty, F. W. Tandem Mass Spectrometry. Science 1981, 214, 280.
Nelson, R. W.; McLean, M. A. Quantitative Determination of Proteins by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Anal. Chem. 1994, 66, 1408–1415.
Muddiman, D. C.; Gusev, A. I.; Proctor, A.; Hercules, D. M. Quantitative Measurement of Cyclosporin A in Blood by Time-of-Flight Mass Spectrometry. Anal. Chem. 1994, 66, 2362–2368.
Yan, W. Y.; Gardella, J. A.; Wood, T. D. Quantitative Analysis of Technical Polymer Mixtures by Matrix Assisted Laser Desorption/Ionization Time-of-Flight-Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2002, 13, 914–920.
Murgasova, R.; Hercules, D. M. Quantitative Characterization of a Polystyrene /Poly(r-methylstyrene) Blend by MALDI Mass Spectrometry and Size-Exclusion Chromatography. Anal. Chem. 2003, 75, 3744–3750.
Chen, H.; He, M. Y.; Pei, J.; He, H. F. Quantitative Analysis of Synthetic Polymers Using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Anal. Chem. 2003, 75, 6531–6535.
Hantonm, S. D.; Owens, K. G. Investigation of Polymer and Matrix Solubility by MALDI and MESIMS. Proceedings of the 46th ASMS Conference on Mass Spectrometry and Allied Topics; Orlando, FL, 1998; p 1185.
Wong, C. K. L.; Chan, T. W. D. Cationization Processes in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry: Attachment of Divalent and Trivalent Metal Ions. Rapid Commun. Mass Spectrom. 1997, 11, 513–519.
Chen, H.; He, M. Y.; Wan, X. H.; Yang, L. F.; He, H. F. The Fragmentation Mechanism Study of Organic Mass Spectrometry. Rapid Commun. Mass Spectrom. 2003, 17, 177–182.
Nielen, M. W. F. MALDI Time-of-Flight Mass Spectrometry of Synthetic Polymers. Mass Spectrom. Rev. 1999, 18, 309–344.
Moghaddam, M. Z.; Heinzle, E.; Tholey, A. Qualitative and Quantitative Analysis of Low Molecular Weight Compounds by Ultraviolet Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Ionic Liquid Matrices. Rapid Commun. Mass Spectrom. 2004, 18, 141–148.
Gusev, A. I.; Wilinson, W. R.; Proctor, A.; Hercules, D. M. Improvement of Ion Signal Reproducibility and Matrix/Comatrix Effects in MALDI Analysis. Anal. Chem. 1995, 67, 1034–1041.
Xiang, F.; Beavis, R. C. A Method to Increase Contaminant Tolerance in Protein MALDI by the Fabrication of Thin Protein-Doped Polycrystalline Films. Rapid Commun. Mass Spectrom. 1994, 8, 199–204.
Hensel, R. R.; King, R. C.; Owens, K. G. Electrospray Sample Preparation for Improved Quantitation in Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Rapid Commun. Mass Spectrom. 1997, 11, 1785–1793.
Lin, Y. S.; Chen, Y. C. Laser Desorption/Ionization Time-of-Flight Mass Spectrometry on Sol-Gel-Derived 2,5-Dihydroxybenzoic Acid Film. Anal. Chem. 2002, 74, 5793–5798.
Wyttenbach, T.; Heleden, von G. Conformations of Alkali Ion Cationized Polyethers in the Gas Phase: Polyethylene Glycol and Bis[(benzo-15-crown -5)-15-ylmethyl]. Int. J. Mass Spectrom. Ion Processes 1997, 165/166, 377.
Cox, F. J.; Johnston, M. V.; Dasgupta, A. Characterization and Relative Ionization Efficiencies of End-Functionalized Polystyrenes by Matrix-Assisted Laser Desorption /Ionization Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2003, 14, 648–657.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published online December 15, 2004
Rights and permissions
About this article
Cite this article
Chen, H., He, M. Quantitation of synthetic polymers using an internal standard by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Am Soc Mass Spectrom 16, 100–106 (2005). https://doi.org/10.1016/j.jasms.2004.09.024
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/j.jasms.2004.09.024