Abstract
A single droplet generator was coupled with a rotating ball inlet matrix-assisted laser desorption/ionization (MALDI) time of flight (TOF) mass spectrometer. Single droplets with 100 picoliter volume were ejected by a piezoelectric-actuated droplet generator and deposited onto a matrix-coated rotating stainless steel ball at atmospheric pressure. The single droplet deposit was transported to the vacuum side of the instrument where ionization was accomplished using a UV pulsed laser. Using this on-line interface, it was possible to obtain protonated molecule signal from as little as 10 fmol analyte.
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Published online September 11, 2004
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Zhang, X., Narcisse, D.A. & Murray, K.K. On-line single droplet deposition for MALDI mass spectrometry. J Am Soc Mass Spectrom 15, 1471–1477 (2004). https://doi.org/10.1016/j.jasms.2004.06.016
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DOI: https://doi.org/10.1016/j.jasms.2004.06.016