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Infrared laser post-ionization of large biomolecules from an IR-MALD(I) plume

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Journal of the American Society for Mass Spectrometry

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Abstract

A two-infrared laser desorption/ionization method is described. A first laser, which was either an Er:YAG laser or an optical parametric oscillator (OPO), served for ablation/vaporization of small volumes of analyte/matrix sample at fluences below the ion detection threshold for direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). A second IR-laser, whose beam intersected the expanding ablation plume at a variable distance and time delay, was used to generate biomolecular ions out of the matrix-assisted laser desorption (MALD) plume. Either one of the two above lasers or an Er:YSGG laser was used for post-ionization. Glycerol was used as IR-MALDI matrix, and mass spectra of peptides, proteins, as well as nucleic acids, some of which in excess of 105 u in molecular weight, were recorded with a time-of-flight mass spectrometer. A mass spectrum of cytochrome c from a water ice matrix is also presented. The MALD plume expansion was investigated by varying the position of the post-ionization laser beam above the glycerol sample surface and its delay time relative to the desorption laser. Comparison between the OPO (pulse duration, τL=6 ns) and the Er:YAG laser (τL∼120 ns) as primary excitation laser demonstrates a significant effect of the laser pulse duration on the MALD process.

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Authors and Affiliations

  1. Institute of Medical Physics and Biophysics, Westfälische-Wilhelms Universität, Münster, Germany

    Arne Leisner, Andreas Rohlfing, Stefan Berkenkamp, Franz Hillenkamp & Klaus Dreisewerd

Authors
  1. Arne Leisner
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  2. Andreas Rohlfing
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  3. Stefan Berkenkamp
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  4. Franz Hillenkamp
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  5. Klaus Dreisewerd
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Corresponding author

Correspondence to Klaus Dreisewerd.

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Published online May 10, 2004

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Leisner, A., Rohlfing, A., Berkenkamp, S. et al. Infrared laser post-ionization of large biomolecules from an IR-MALD(I) plume. J Am Soc Mass Spectrom 15, 934–941 (2004). https://doi.org/10.1016/j.jasms.2004.03.010

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  • DOI: https://doi.org/10.1016/j.jasms.2004.03.010

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