Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry was used to obtain spectra of peptide-DNA complexes formed by basic domain (BD15) of c-Fos protein and DNA AP-1 site (5′-TGAGTCA-3′). The noncovalent interaction between single stranded DNA and BD15 was observed and confirmed to be an ionic one between the negatively charged sugar-phosphate backbone of DNA and positively charged side chains of Arg-and lys-rich peptides as demonstrated by Vertes and coworkers [1] and Woods and coworkers [2]. But the specific noncovalent interaction between DNA AP-1 site and the dimer of BD15 was firstly detected in this paper. Various different sequence DNAs were studied and it was found that this interaction is a sequence-specific one, and AP-1 site was essential for this interaction. This specific interaction depends on the matrix. It was only observed in the ATT matrix and not in the other two matrixes (CHCA and DHBA).
Article PDF
Avoid common mistakes on your manuscript.
References
Tang, X.; Callahan, J. H.; Zhou, P.; Vertes, A.; Anal. Chem. 1995, 67, 4542–4548.
Lin, S.; Cotter, R. J.; Woods, A. S.; Prot. Struct. Funct. Genet. 1998, 2(Suppl.), 12–21.
Mcbride, K.; Nemer, M.; Mol. Cell. Biol. 1998, 9(18), 5073–5081.
Cheng, X. H.; Harms, A. C.; Goudreau, P. N.; Terwilliger, T. C.; Smith, R. D.; Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 7022–7027.
Hsieh, Y. L.; Li, Y. T.; Henion, J. D.; Ga–nem, B.; Biol. J. Mass. Spectrom. 1994, 23, 272–276.
Woods, A. S.; Koomen, J. M.; Ruotolo, B. T.; Gillig, K. J.; Russel, D. H.; Fuhrer, K.; Gonin, M.; Egan, T. F.; Schultz, J. A.; J. Am. Soc. Mass. Spectrom. 2002, 13, 166–169.
Krutchinsky, A. N.; Zhang, W. Z.; Chait, B. T.; J. Am. Soc. Mass. Spectrom. 2000, 11, 493–504.
Dai, Y. Q.; Whittal, R. M.; Li, L.; Anal. Chem. 1999, 71, 1087–1091.
Kim, Y.; Hurst, G. B.; Microchem. J. 2001, 71, 219–228.
Anfinsen, C. B.; Science 1973, 181, 223–230.
Farmer, T. B.; Caprioli, R. M.; J. Mass. Spectrom. 1998, 33, 697–704.
Cheng, X. H.; Morin, P. E.; Harms, A. C.; Bruce, J. E.; BenDavid, Y.; Smith, R. D.; Anal. Chem. 1996, 239, 35–40.
Woods, A. S.; Buchsbaum, J. C.; Worrall, T. A.; Berg, M. J.; Cotter, R. J.; Anal. Chem. 1995, 67, 4462–4465.
Lecchi, P.; Pannell, L. K.; J. Am. Soc. Mass. Spectrom. 1995, 6, 972–975.
Juhasz, P.; Biemann, K.; Proc. Natl. Acad. Sci. U. S. A. 1994, 91, 4333–4337.
Lin, S. H.; Long, S.; Ramirez, S. M.; Cotter, R. J.; Woods, A. S.; Anal. Chem. 2000, 72, 2635–2640.
Blenis, J.; Proc. Natl. Acad. Sci. U. S. A. 1993, 90, 5889–5892.
Chiu, R.; Boyle, W. J.; Meek, J.; Smeal, T.; Hunter, T.; Karin, M.; Cell 1988, 54M, 541–552.
Glover, J. N. M.; Harrison, S. C.; Nature 1995, 373, 257–261.
Yamgaki, T.; Ishizuka, Y.; Kawabata, S.; Nakanishi, H.; Rapid. Commun. Mass Spectrom. 1997, 11, 527–531.
Franza, B. R.; Sambucetti, L. C.; Cohen, D. R.; Curran, T.; Oncogene 1987, 1, 213–221.
Author information
Authors and Affiliations
Corresponding author
Additional information
Published online November 4, 2003
Rights and permissions
About this article
Cite this article
Luo, S.Z., Li, Y.M., Qiang, W. et al. Detection of specific noncovalent interaction of peptide with DNA by MALDI-TOF. J Am Soc Mass Spectrom 15, 28–31 (2004). https://doi.org/10.1016/j.jasms.2003.08.008
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1016/j.jasms.2003.08.008