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A new free radical scavenger, edaravone, ameliorates oxidative liver damage due to ischemia-reperfusion in vitro and in vivo

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Journal of Gastrointestinal Surgery

Abstract

Ischemia-reperfusion injury causes oxidative stress producing reactive oxygen species, which is a serious problem linked to morbidity and mortality in liver surgery. We investigated the effects of edaravone, a new free radical scavenger, on liver oxidative stress in vitro and in vivo. We employed a hypoxiareoxygenation model of primary cultured hepatocytes using an AnaeroPack (Mitsubishi Gas Chemical Co., Tokyo, Japan). Hepatocytes were exposed to 3 or 4 hours of hypoxia and then returned to oxygenation. We analyzed the time course changes of aspartate aminotransferase (AST), phosphatidylcholine hydroperoxide (PCOOH), and adenosine triphosphate (ATP) content in hepatocytes of edaravone-treated groups or nontreated groups after reoxygenation. Edaravone significantly attenuated the elevation of the AST level of the medium and hepatocellular PCOOH and preserved the hepatocellular ATP level. In vivo, male Sprague-Dawley rats were subjected to 45 minutes of hepatic ischemia and 120 minutes of reperfusion. The rats were intravenously injected with vehicle or edaravone (3 mg/kg or 10 mg/kg) before reperfusion and 1 hour after reperfusion. Serum AST levels and hepatic PCOOH and energy charge were significantly improved in both edaravone groups compared with control. In conclusion, edaravone has the ability to eliminate intra-hepatocellular superoxide species and attenuate oxidative liver damage in liver surgery.

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Authors and Affiliations

  1. Division of Gastroenterological Surgery, Department of Surgery, Tohoku University Graduate School of Medicine, 1-1, Seiryo-machi, Aoba-ku, Sendai, 980-8574, Miyagi, Japan

    Tomoya Abe M.D., Michiaki Unno M.D., Ph.D., Heigo Takeuchi M.D., Ph.D., Tetsuya Kakita M.D., Ph.D., Yu Katayose M.D., Ph.D., Toshiki Rikiyama M.D., Ph.D., Takanori Morikawa M.D., Ph.D., Masanori Suzuki M.D., Ph.D. & Seiki Matsuno M.D., Ph.D., F.A.C.S.

Authors
  1. Tomoya Abe M.D.
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  2. Michiaki Unno M.D., Ph.D.
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  3. Heigo Takeuchi M.D., Ph.D.
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  4. Tetsuya Kakita M.D., Ph.D.
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  5. Yu Katayose M.D., Ph.D.
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  6. Toshiki Rikiyama M.D., Ph.D.
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  7. Takanori Morikawa M.D., Ph.D.
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  8. Masanori Suzuki M.D., Ph.D.
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  9. Seiki Matsuno M.D., Ph.D., F.A.C.S.
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Corresponding author

Correspondence to Michiaki Unno M.D., Ph.D..

Additional information

This work was supported in part by research grants from the Ministry of Education, Science, and Culture of Japan and the Yamanouchi Foundation.

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Abe, T., Unno, M., Takeuchi, H. et al. A new free radical scavenger, edaravone, ameliorates oxidative liver damage due to ischemia-reperfusion in vitro and in vivo. J Gastrointest Surg 8, 604–615 (2004). https://doi.org/10.1016/j.gassur.2004.02.011

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  • DOI: https://doi.org/10.1016/j.gassur.2004.02.011

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