Abstract
Spathulenol was isolated from an extract of Azorella compacta Phil., Apiaceae, by various chromatographic method; identification of the chemical structure was confirmed by comparing its spectroscopic data with those reported in the literature. The anti-Mycobacterium tuberculosis activity of spathulenol was evaluated on MDR, pre-XDR, and XDR clinical isolates of M. tuberculosis, as well as on the reference susceptible strain H37Rv and its cytotoxic activity was evaluated on the Vero Cell Line. The anti-M. tuberculosis activity of spathulenol was twice as potent against the MDR, pre-XDR, and XDR clinical isolates (6.25 μg/ml) than on the susceptible H37Rv strain (12.5 μg/ml). Additionally, the anti-M. tuberculosis activity shown by spathulenol was established as bactericidal on drug-resistant and susceptible strains of M. tuberculosis. Finally, cytotoxic activity on the Vero cell line (CC50 = 95.7 μg/ml) indicated that spathulenol is a selective anti-M. tuberculosis compound, with a selective index of 15.31 against drug-resistant clinical isolates of M. tuberculosis.
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GMMS and LMPR contributed to conceptualization of the study; AHUC, LMPR and GMMS contributed to formal analysis; JB and GMMS contributed to funding acquisition; All authors contribute to investigation and methodology; GMMS contributed to project administration; JB, LAL and GMMS contributed to resources; AHUC and GMMS contributed to writing the original draft and all authors writing - review & editing the manuscript.
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de Dzul-Beh, A.J., García-Sosa, K., Uc-Cachón, A.H. et al. In vitro growth inhibition and bactericidal activity of spathulenol against drug-resistant clinical isolates of Mycobacterium tuberculosis. Rev. Bras. Farmacogn. 29, 798–800 (2019). https://doi.org/10.1016/j.bjp.2019.06.001
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DOI: https://doi.org/10.1016/j.bjp.2019.06.001