Abstract
The “arnicas” found in Brazil are examples of different species of the family Asteraceae used in popular medicine for its attributed anti-inflammatory action. Among the species known and used as “arnica” we selected: Calea uniflora Less., Chaptalia nutans (L.) Polák, Lychnophora ericoides Mart., Lychnophora pinaster Mart., Lychnophora salicifolia Mart., Lychnophora diamantinana Coile & S.B.Jones, Porophyllum ruderale (Jacq.) Cass., Pseudobrickellia brasiliensis (Spreng.) R.M.King & H.Rob., Sphagneticola trilobata (L.) Pruski, and Solidago chilensis Meyen, due to their extensive use. This research provides new information on leaf morphology and anatomy and on chemistry of the major metabolites found in these species through histochemical tests and phytochemical review. The results revealed anatomical characters for the differentiation and quality control of the vegetal drugs, being these: distinctive epidermal attachments, epidermis cells, parenchymal cells of the mesophyll, vascular bundles, midvein patterns and secretory structures of exudation of secondary metabolites. The review of chemical profiles showed differences in the chemical composition of the species, as different skeletons of sesquiterpene lactones in the species evaluated in addition to other chemical classes such as terpenes, flavonoids, chromenes and phenolic acids derivate. Based on the results obtained in this work it is important to emphasize that the information about the ten species of arnica generate subsidies for differentiation and identification of characteristic markers and for the diagnosis of the species and it can be applied in the “arnicas” quality control.
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Abreu, V.C.G., Takahashi, J.A., Duarte, LP., Piló-Veloso, D., Junior, P.A.S., Alves, D., Romanha, A.J., Alcântara, A.F., 2011. Evaluation of the bactericidal and trypanocidal activities of triterpenes isolated from the leaves, stems, and flowers of Lychnophora pinaster. Rev. Bras. Farmacogn. 21, 615–621.
Alfredo, P.P., Anaruma, CA., Pião, A.C.S., João, S.M.A., Casarotto, R.A., 2008. Qualitative analysis of effects of phonophoresis with Arnica montana onto acute inflammatory process in rat skeletal muscles. Fisioter. Pesqui. 15, 273–279.
Almeida, V.G., Avelar-Freitas, B.A., Santos, M.G., Costa, LA., Silva, T.J., Pereira, W.F., Amorim, MLL., Grael, C.F.F., Gregório, LE., Rochavieira, E., Brito-Mel, G.E.A., 2017. Inhibitory effect of the Pseudobrickellia brasiliensis (Spreng) R.M King & H. Rob. aqueous extract on human lymphocyte proliferation and IFN-γ and TNF-α production in vitro. Braz. J. Med. Biol. Res. 50, 1–8.
Amorim, MLL., Godinho, W.M., Archanjo, F.C., Grael, C.F.F., 2016. Chemical constituents of Pseudobrickellia brasiliensis leaves (Spreng.) R.M. King & H. Rob. (Asteraceae). Rev. Bras. Plantas Med. 18, 408–414.
Baccarin, T., Czepula, A.I., Ferreira, R.A., Lucinda, R.M., 2009. Análise morfoanatômica das partes aéreas de Wedelia paludosa DC. (Acmela brasiliensis, Sphagneticola trilobata), Asteraceae. Rev. Bras. Farmacogn. 19, 612–616.
Badilla, B., Mora, G., Poveda, L.J., 1999. Antiinflammatory activity of aqueous extracts of five Costa Rican medicinal plants in Sprague-Dawley rats. Rev. Biol. Trop. 47, 723–727.
Batista, R., García, P.A., Castro, M.A., Corral, J.M., Feliciano, A., Oliveira, A., 2010. Iso-Kaurenoic acid from Wedelia paludosa D.C. An. Acad. Bras. Cienc. 82, 823–831.
Bohlmann, F., Zdero, C., King, R.M., Robinson, H., 1984. A hydroxygermacrene and other constituents from Pseudobrickellia brasiliensis. Phytochemistry 23, 1798–1799.
Borella, J.C., Lopes, J.L., Vichnewski, W., Cunha, W.R., Herz, W., 1998. Sesquiterpene lactones, triterpenes and flavones from Lychnophora ericoides and Lychnophora pseudovillosissima. Biochem. Syst. Ecol. 26, 671–676.
Brazil, 2010. FarmacopéiaBrasileira, 5a ed. Agência Nacional de Vigilância Sanitária, Brasília, pp. 546.
Budel, J.M., Duarte, M.R., Farago, P.V., Takeda, I.J.M., 2006. Caracteres anatômicos de folha e caule de Calea uniflora Less., Asteraceae. Rev. Bras. Farmacogn. 16, 53–60.
Carvalho, G.J.A., Carvalho, M.G., Ferreira, D.T., Faria, T.J., Braz-Filho, R., 2001. Diter-penos, triterpenos e esteróides das flores de Wedelia paludosa. Quim. Nova 24, 24–26.
Cechinel-Filho, V., Block, L.C., Yunes, RA, Monache, F.D., 2004. Paludolactone: Anew Eudesmanolide Lactone from Wedelia Paludosa Dc. (Acmela Brasiliensis). Nat. Prod. Res. 18, 47–451.
Chadwick, M., Trewin, H., Gawthrop, F., Wagstaff, C., 2013. Sesquiterpenoids lactones: benefits to plants and people. Int. J. Mol. Sci. 14, 12780–12805.
Duarte, M.R., Siebenrok, M.C.N., Empinotti, C.B., 2007. Anatomia comparada de espécies de arnica: Porophyllum ruderale (Jacq.) Cass. e Chaptalia nutans (L) Pohl Rev. Cienc. Farm. Basica Apl. 28, 193–201.
Empinotti, C.B., Duarte, M.R., 2006. Caracteres anatômicos de arnica-do-campo: Chaptalia nutans. Acta Farm. Bonaer. 25, 333–338.
Fernandes, Y.S., Trindade, L.M.P., Rezende, M.H., Paula, J.R., Gonçalves, L.A., 2016. Trichomes and chemical composition of the volatile oil of Trichogonia cinerea (Gardner) R.M. King & H. Rob. (Eupatorieae Asteraceae). An. Acad. Bras. Cienc. 88, 309–322.
Ferreira, A.A., Azevedo, A.O., Silveira, D., Oliveira, P.M., Castro, M.S.A., Raslan, D.S., 2005. Constituents of Lychnophora pinaster hydroalcoholic extract. Chem. Nat. Comp. 41, 466.
Fonseca, M.C.M., Meira, R.M.S.A., Casali, V.W.D., 2006. Anatomia dos órgãos vege-tativos e histolocalização de compostos fenólicos e lipídicos em Porophyllum ruderale (Asteraceae). Planta Daninha 24, 707–713.
Freires, I.A., Alves, L.A., Jovito, V.C., Almeida, L.F.D., Castro, R.D., Padilha, W.W.N., 2010. In vitro antibacterial and antiadherent activities of tinctures from Schinus terebinthifolius (Aroeira) and Solidago microglossa (Arnica) on dental biofilm forming bacteria. Odontol. Clín.-Cient. 9, 139–143.
Gastaldi, B., Catalán, CA., Silva-sofrás, F.M., González, S.B., 2018. Solidago chilensis Meyen (Asteraceae), a medicinal plant from South America. A comprehensive review: ethnomedicinal uses, phytochemistry and bioactivity. B. Latinoam. Caribe Pl. 17., pp. 17–29.
Geissman, TA., Griffin, T.S., 1971. Sesquiterpene lactones: acid-catalyzed color reactions as an aid in structure determination. Phytochemistry 10, 2475–2485.
Gobbo-Neto, L., Santos, M.D., Kanashiro, A., Almeida, M.C., Lucisano-Valim, Y.M., Lopes, JLC., Souza, G.E.P., Lopes, N.P., 2005. Evaluation of the anti-Inflammatory and antioxidant Activities of di-C-glucosylflavones from Lychnophora ericoides (Asteraceae). Planta Med. 71, 3–6.
Gouvea, D.R., Meloni, F., Ribeiro, A.B.B., Lopes, J.L.C., Lopes, N.P., 2012. A new HPLC-DAD-MS/MS method for the simultaneous determination of major compounds in the crude extract of Lychnophora salicifolia Mart. (Brazilian arnicão) leaves: application to chemical variability evaluation. Anal. Chim. Acta 748, 28–36.
Guillet, G., BełLanger, A., Arnason, J.T., 1998. Volatile monoterpenes in Porophyllum gracile and P. ruderale (Asteraceae): identification, localization and insecticidal synergism with α-terthienyl. Phytochemistry 49, 423–429.
Jensen, WA., 1962. Botanical Histochemistry (Principles and Pratice). W.H. Freeman and Company, San Francisco.
Johansen, DA., 1940. Plant Microtechnique. Mc Graw Hill Book, New York.
Keles, L.C., Gianasi, F.M., Souza, R.C., Brito, B.L., Schaab, E.H., Souza, M.G.M., Carvalho, T.C., Martins, C.H.G., Veneziani, R.C.S., Cunha, W.R., Crotti, A.E.M., 2011. Antibacterial activity of 15-deoxygoyazensolide isolated from the stems of Minasia alpestris (Asteraceae) against oral pathogens. Nat. Prod. Res. 25, 326–331.
Kowalski, R., Sugier, D., Sugier, P., Kolodzij, B., 2015. Evaluation of the chemical composition of essential oils with respect to the maturity of flower heads of Arnica montana L and Arnica chamissonis Less. cultivated for industry. Ind. Crop. Prod. 76, 857–865.
Lang, K., Corrêa, J., Wolff, F., Da Silva, G.F., Malheiros, A., Filho, V.C., Silva, R.M.L., Quintão, N.L.M., Sandjo, L.P., Bonomini, T.J., Bresolin, T.M.B., 2017. Biomonitored UHPLC-ESI-QTOF-MS and HPLC-UV thermostability study of the aerial parts of Sphagneticola trilobata (L) Pruski, Asteraceae. Talanta 167, 302–309.
Lima, T.C., Souza, R.J., Moraes, M.H., Steindel, M., Biavatti, M.W., 2017. New furanohe-liangolide sesquiterpene lactone from Caleapinnatifida (R Br.) Less. (Asteraceae) and evaluation of its trypanocidal and leishmanicidal activities. J. Braz. Chem. Soc. 28, 367–375.
Lima, T.C., Souza, R.J., Santos, A.D.C., Moraes, M.H., Biondo, N.E., Barison, A., Steindel, M., Biavatti, M.W., 2015. Evaluation of leishmanicidal and trypanocidal activities of phenolic compounds from Calea uniflora Less. Nat. Prod. Res. 30, 551–557.
Lima-Neto, DA Estudo fitoquímico e efeitos analgésicos da Porophyllum ruderale, 1996. PhD thesis, Curso de Farmacologia, Universidade Estadual de Campinas, Piracicaba.
Lusa, M.G., Costa, F.B., Appezzato-da-Gloria, B., 2016a. Histolocalization of chemo-taxonomic markers in Brazilian Vernonieae (Asteraceae). Bot. J. Linn. Soc. 182, 581–593.
Lusa, M.G., Martucci, M.E.P., Loeuille, B.F.P., Gobbo-Neto, L., Appezzato-da-Glória, B., Costa, F.B., 2016b. Characterization and evolution of secondary metabolites in Brazilian Vernonieae (Asteraceae) assessed by LC-MS fingerprinting. Bot. J. Linn. Soc. 182, 594–661.
Marin, R., 2014. Solidago chilensis Meyer: desenvolvimento de métodos analíti-cos, extratos secos qualificados, avaliação farmacológica in vivo e produção de comprimidos. PhD Thesis, Programa em Ciencias Farmaceuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre.
Marques, M., 2006. Estudo da resposta imunológica induzida por Arnica montana L. PhD Thesis. Programa em Ciências Farmacêuticas, Biociências e Biotecnologia Aplicadas à Farmácia, Universidade Estadual Paulista, Araraquara.
Metcalfe, CR., Chalk, L., 1988. Anatomy of Dicotyledons, 2nd ed. Clarendon Press. US, Oxford.
Milan, P., Hayashi, A.H., Appezzato-da-Glória, B., 2006. Comparative leaf morphology and anatomy of three Asteraceae species. Braz. Arch. Biol. Techn. 49, 135–144.
Nascimento, A.M., Costa, F.C., Thiemann, O.H., Oliveira, D.C.R., 2007. Chromanones with leishmanicidal activity from Calea uniflora. Z. Naturforsch. C 62, 353–356.
Obón, C., Rivera, D., Verde, A., Fajardo, J., Valdes, A., Alcaraz, F., Carvalho, A.M.M., 2012. Arnica: a multivariate analysis of the botany and ethnopharmacology of a medicinal plant complex in the Iberian Peninsula and the Balearic Islands. J. Ethnopharmacol. 144, 44–56.
Prudêncio, R., 2012. Levantamento etnofarmacológico de Solidago chilensis Meyen. “Arnica-brasileira” (Asteraceae). Especialista em Ecologia e Manejo de Recursos Naturais, Universidade do Extremo Sul Catarinense, Criciúma.
Raggi, L., 2013. Teor, composição química e atividade biológica de óleos voláteis de Sphagneticola trilobata (L.) Pruski e Porophyllum ruderale (Jacq.) Cass. (Asteraceae). PhD Thesis, Programa - Biodiversidade Vegetal e Meio Ambiente, Área de Concentração de Plantas Vasculares em Análises Ambientais, Instituto de Botânica da Secretaria do Meio Ambiente.
Rodríguez-Chávez, J.L., Egas, V., Linares, E., Bye, R., Hernández, T., Espinosa-García, F.J., Delgado, G., 2017. Mexican arnica (Heterotheca inuloides Cass Asteraceae: Astereae). J. Ethnopharmacol. 195, 39–63.
Sabir, S.M., Ahmad, S.D., Hamid, A., Khan, M.Q., Athayde, M.L., Santos, D.B., Boligon, A.A., Rocha, J.B.T., 2012. Antioxidant and hepatoprotective activity of ethanolic extract of leaves of Solidago microglossa containing polyphenolic compounds. Food Chem. 131, 741–747.
Sakai, W.S., 1973. Simple method for differential staining of paraffinn embedded -plant material using toluidine blue. Stain Technol. 43, 247–249.
Sakamoto, H.T., Flausino, D., Castellano, E.E., Stark, C.B.W., Gates, P.J., Lopes, N.P., 2003. Sesquiterpene lactones from Lychnophora ericoides. J. Nat. Prod. 66, 695–963.
Sales, M.D.C., Sartor, E.B., Gentilli, R.M., 2015. Ethnobotany and ethnopharmacol- ogy: traditional medicine and the bioprospection of phytotherapics. Salus J. Res. Health Sci. 1, 17–25.
Sandini, T.M., Udo, M.S.B., Spinosa, H.S., 2013. Senecio brasiliensis e alcaloides pirrolizidínicos: toxicidade em animais e na saúde humana. Biotemas 26, 83–92.
Schmeda-Hirschmann, G., Rodriguez, J., Astudillo, L., 2002. Gastroprotective activity of the diterpene solidagenone and its derivatives on experimentally induced gastric lesions in mice. J. Ethnopharmacol. 81, 111–115.
Semir, J., Rezende, A.R., Monge, M., Lopes, N.P., 2011. As arnicas endêmicas das serras do Brasil: Uma revisão sobre a biologia e a química das espécies de Lychnophora (Asteraceae), 1st ed. UFOP, Ouro Preto, MG.
Silva, P.S.S., Linhares, J.F.P., Marques, M.O.M., 2013. Caracterização morfológica, perfil químico, atividade biológica e conservação in situ do gênero Lychnophora Mart. (Asteraceae: Vernonieae: Lychnophorinae), Brasil. Biotemas 26, 201–203.
Smith, F.H., Smith, E.C., 1942. Anatomy of the inferior ovary of Darbya. Am. J. Bot. 29, 464–471.
Souza, D.M.F., Souza, R.D., Sá, E.L., Araújo, Randau, K.P., 2017. Anatomical, phyto-chemical and histochemical study of Solidago chilensis Meyen. An. Acad. Bras. Cienc. 90, 2107–2120.
Spring, O., Zipper, R., Reeb, S., Vogler, B., Da Costa, F.B., 2001. Sesquiterpene lactones and a myoinositol from glandular trichomes of Viguiera quinqueremis (Heliantheae; Asteraceae). Phytochemistry 57, 267–272.
Takahashi, H.T., Novello, CR., Ueda-Nakamura, T., Dias Filho, B.P., Palazzo De Mello, J.C., Nakamura, C.V., 2011. Thiophene derivatives with antileishmanial activity isolated from aerial parts of Porophyllum ruderale (Jacq.) Cass. Molecules 16, 3469–3478.
Tamura, E.K., Jimenez, R.S., Waismam, K., Gobbo-Neto, L., Lopes, N.P., Malpezzi-Marinho, E.A.L., Marinho, E.A.V., Farsky, S.H.P., 2009. Inhibitory effects of Solidago chilensis Meyen hydroalcoholic extract on acute inflammation. J. Ethnopharmacol. 122, 478–485.
Truiti, M.C.T., Sarragiotto, M.H., Filho, B.A.A., Nakamura, C.V., Dias Filho, B.P., 2003. In vitro antibacterial activity of a 7-O-ß-D-glucopyranosyl-nutanocouMarin from Chaptalia nutans (Asteraceae). Mem. I. Oswaldo Cruz 98, 283–286.
Valverde, S.S., Oliveira, T.B., Souza, S.P., 2012. Solidago chilensis Meyen (Asteraceae). Rev. Fitos 7, 131–136.
Vechia, C.A.D., Morais, B., Schonell, A.P., Diel, K.A.P., Faust, C., Menin, C., Gomes, D.B., Roman Junior, W.A., 2016. Isolamento químico e validação analítica por cro-matografia líquida de alta eficiência de quercitrina em Solidago chilensis Meyen (Asteraceae). Rev. Bras. Pl. Med. 18, 288–296.
Verma, R.S., Padalia, R.C., Chauhan, A., Sundaresan, V., 2013. Essential oil composition of Sphagneticola trilobata (L.) Pruski from India. J. Essent. Oil Res. 26, 29–33.
Wildner, L.M., 2016. Atividade antimicobacteriana in vitro de lactonas sesquiter-pênicas e investigação do seu mecanismo de ação. PhD Thesis. Programa de Pós-graduação em Farmácia, Universidade Federal de Santa Catarina. Florianópolis.
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AEA collected the plants, carried out the anatomical analysis and wrote the manuscript. ER and JW performed the anatomical analysis. MGL and MWB created the project and supervised the laboratory work. All the authors have read the final manuscript and approved the submission.
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de Athayde, A.E., Richetti, E., Wolff, J. et al. “Arnicas” from Brazil: comparative analysis among ten species. Rev. Bras. Farmacogn. 29, 401–424 (2019). https://doi.org/10.1016/j.bjp.2019.02.006
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DOI: https://doi.org/10.1016/j.bjp.2019.02.006