Abstract
In this study, the adsorption/desorption characteristics of quercetin, luteolin and apigenin from Flos populi extract (Populus tomentosa Carrière, Salicaceae) on twelve macroporous resins (NKA-9, HPD-600, HPD-826, HPD-750, HPD-400, DM-130, AB-8, SP-825, X-5, D-101, HPD-100, HPD-200) were evaluated. Both high adsorption and desorption capacities of quercetin, luteolin and apigenin from Flos populi extract on SP-825 resin indicated that SP-825 resin was appropriate and its data were well fitted to the Langmuir and Freundlich isotherms. To get the optimal separation process, the influences of factors such as flow rates, loading sample volumes, concentrations of desorption solution were further investigated. Column packed with SP-825 resin was used to perform dynamic adsorption and desorption experiments. After one round of treatment, the contents of quercetin, luteolin and apigenin in the final products were 3.75-fold, 3.67-fold and 3.54-fold increased with recovery yields of 87.25, 85.19 and 82.22%, respectively. The results showed that the preparative enrichment of quercetin, luteolin and apigenin was available via adsorption and desorption on SP-825 resin. This method is a promising basis for the large-scale preparation of quercetin, luteolin and apigenin from Flos populi.
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Amelio, A., Loise, L., Azhandeh, R., Darvishmanesh, S., Calabro, V., Degreve, J., Luis, P., Van der Bruggen, B., 2016. Purification of biodiesel using a membrane contactor: liquid-liquid extraction. Fuel Process. Technol. 142, 352–360.
Chang, X.L., Wang, D., Chen, B.Y., Feng, Y.M., Wen, S.H., Zhan, P.Y., 2012. Adsorption and desorption properties of macroporous resins for anthocyanins from the calyx extract of roselle (Hibiscus sabdariffa L). J. Agric. Food Chem. 60, 2368–2376.
Chinese Pharmacopoeia, 2015. Pharmacopoeia of People’ s Republic of China. Chemical Industry Press, Beijing.
Chiow, K.H., Phoon, M.C., Putti, T., Tan, B.K.H., Chow, V.T., 2016. Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserinon murine coronavirus and dengue virus infection. Asian Pac. J. Trop. Med. 9, 1–7.
Du, Z., Wang, K., Tao, Y., Chen, L., Qiu, F., 2012. Purification of baicalin and wogonoside from Scutellaria baicalensis extracts by macroporous resin adsorption chromatography. J. Chromatogr. B 908, 143–149.
Feng, W.M., Guo, H.H., Xue, T., Wang, X., Tang, C.W., Ying, B., Gong, H., Cui, G., 2015. Anti-inflammation and anti-fibrosis with PEGylated, apigenin loaded PLGA nanoparticles in chronic pancreatitis disease. RSC Adv. 5, 83628–83635.
Li, Y., Liu, J., Cao, R., Deng, S., Lu, X., 2013. Adsorption of myricetrin, puerarin, naringin, rutin, and neohesperidin dihydrochalcone flavonoids on macroporous resins. J. Chem. Eng. Data 58, 2527–2537.
Lin, L., Zhao, H., Dong, Y., Yang, B., Zhao, M., 2012. Macroporous resin purification behavior of phenolics and rosmarinic acid from Rabdosia serra (MAXIM.) HARA leaf. Food Chem. 130, 417–424.
Liu, B., Dong, B., Yuan, X., Kuang, Q., Zhao, Q., Yang, M., Liu, J., Zhao, B., 2016. Enrichment and separation of chlorogenic acid from the extract of Eupa-torium adenophorum Spreng by macroporous resin. J. Chromatogr. B 1008, 58–64.
Liu, W., Zhang, S., Zu, Y.G., Fu, Y.-J., Ma, W., Zhang, D.Y., Kong, Y., Li, X.-J., 2010a. Preliminary enrichment and separation of genistein and apigenin from extracts of pigeon pea roots by macroporous resins. Bioresource Technol. 101, 4667–4675.
Liu, Y.F., Liu, J.X., Chen, X.F., Liu, Y.W., Di, D.L., 2010b. Preparative separation and purification of lycopene from tomato skins extracts by macroporous adsorption resins. Food Chem. 123, 1027–1034.
Liu, Z., Wang, J.Y., Gao, W.Y., Man, S.L., Wang, Y., Liu, C.X., 2013. Preparative separation and purification of steroidal saponins in Paris polyphylla var. yunnanensis by macroporous adsorption resins. Pharm. Biol. 51, 899–905.
Mun, L.W., Rafiqul, I.S.M., Sakinah, A.M.M., Zularisam, A.W., 2016. Purification of bioxylitol by liquid-liquid extraction from enzymatic reaction mixture. Sep. Sci. Technol. 51, 2369–2377.
Sandhu, A.K., Gu, L., 2013. Adsorption/desorption characteristics and separation of anthocyanins from muscadine (Vitis rotundifolia) juice pomace by use of macroporous adsorbent resins. J. Agric. Food Chem. 61, 1441–1448.
Shao, H.J., Jing, K., Mahmoud, E., Huang, H.H., Fang, X.J., Yu, C.R., 2013. Apigenin sensitizes colon cancer cells to antitumor activity of ABT-263. Mol. Cancer Ther. 12, 2640–2650.
Si, C.L., Wu, L., Ni, Y.H., 2010. Isolation and structure elucidation of low molecular weight extractives from wood of triploid Populus tomentosa Carr. In: Research Progress in Paper Industry and Biorefinery (4th Isetpp), vols. 1–3, pp. 197–200.
Smith, N.W., Evans, M.B., 1995. Efficient analysis of neutral and highly polar pharmaceutical compounds using reversed-phase and ion-exchange electrochromatography. Chromatographia 41, 197–203.
Sun, P.C., Liu, Y., Yi, Y.T., Li, H.J., Fan, P., Xia, C.H., 2015. Preliminary enrichment and separation of chlorogenic acid from Helianthus tuberosus L. leaves extract by macroporous resins. Food Chem. 168, 55–62.
Torres, S., Cerutti, S., Raba, J., Pacheco, P., Silva, M.F., 2014. Preconcentration of seleno-amino acids on a XAD resin and determination in regional olive oils by SPE UPLC-ESI-MS/MS. Food Chem. 159, 407–413.
Wang, B., Goldsmith, C.D., Zhao, J., Zhao, S., Sheng, Z., Yu, W., 2018. Optimization of ultrasound-assisted extraction of quercetin, luteolin, apigenin, pinocembrin and chrysin from Flos populi by Plackett-Burman design combined with Taguchi method. Chiang Mai J. Sci. 45, 427–439.
Wu, W., Li, R., Li, X., He, J., Jiang, S., Liu, S., Yang, J., 2015. Quercetin as an antiviral agent inhibits Influenza A virus (IAV) entry. Viruses, 8.
Xi, L., Mu, T., Sun, H., 2015. Preparative purification of polyphenols from sweet potato (Ipomoea batatas L.) leaves by AB-8 macroporous resins. Food Chem. 172, 166–174.
Xu, H.L., Yang, T., Liu, X.H., Tian, Y., Chen, X.L., Yuan, R., Su, S.N., Lin, X.K., Du, G.H., 2016. Luteolin synergizes the antitumor effects of 5-fluorouracil against human hepatocellular carcinoma cells through apoptosis induction and metabolism. Life Sci. 144, 138–147.
Xu, Q., Shen, Z., Wang, Y., Guo, S., Li, F., Wang, Y., Zhou, C., 2013. Anti-diarrhoeal and antimicrobial activity of Flos populi (male inflorescence of Populus tomentosa Carrière) aqueous extracts. J. Ethnopharmacol. 148, 640–646.
Xu, Q., Wang, Y., Guo, S., Shen, Z., Wang, Y., Yang, L., 2014. Anti-inflammatory and analgesic activity of aqueous extract of Flos populi. J. Ethnopharmacol. 152, 540–545.
Yang, Q., Zhao, M., Lin, L., 2016. Adsorption and desorption characteristics of adlay bran free phenolics on macroporous resins. Food Chem. 194, 900–907.
Zhang, B., Yang, R.Y., Zhao, Y., Liu, C.Z., 2008. Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins. J. Chromatogr. B 867, 253–258.
Zhao, P., Qi, C., Wang, G., Dai, X., Hou, X., 2015. Enrichment and purification of total flavonoids from Cortex juglandis Mandshuricae extracts and their suppressive effect on carbon tetrachloride-induced hepatic injury in mice. J. Chromatogr. B 1007, 8–17.
Zhao, Y., Tang, G.S., Cai, E.B., Liu, S.L., Zhang, L.X., Wang, S.J., 2014. Hypolipidaemic and antioxidant properties of ethanol extract from Flospopuli. Nat. Prod. Res. 28, 1467–1470.
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YZ and BW carried out most of the studies. ZS designed the study and wrote the manuscript. CS improved the manuscript language.All authors have read and approved the final version.
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Zhang, Y., Wang, B., Jia, Z. et al. Adsorption/desorption characteristics and enrichment of quercetin, luteolin and apigenin from Flos populi using macroporous resin. Rev. Bras. Farmacogn. 29, 69–76 (2019). https://doi.org/10.1016/j.bjp.2018.09.002
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DOI: https://doi.org/10.1016/j.bjp.2018.09.002